GLR Antibody | Affinity Biosciences

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Western blot analysis of GLR expression in HUVEC cell lysates.

AF5319 at 1/100 staining Human colorectal cancer by IHC-P.

AF5319 at 1/100 staining Rat stomach tissue by IHC-P.

Product:	GLR Antibody
Catalog:	AF5319
Description:	Rabbit polyclonal antibody to GLR
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Dog
Mol.Wt.:	56 kDa; 54kD(Calculated).
Uniprot:	P47871
RRID:	AB_2837804

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(83%), Horse(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

GLR Antibody detects endogenous levels of total GLR.

RRID:

AB_2837804
Cite Format: Affinity Biosciences Cat# AF5319, RRID:AB_2837804.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Gcgr; GGR; GL R; GL-R; GLR; GLR_HUMAN; Glucagon receptor; Glucagon receptor precursor; MGC138246;

Immunogens

Immunogen:

Uniprot:

P47871(GLR_HUMAN) >>Visit HPA database.

Gene(ID):

GCGR(2642)

Description:

GLRB (Glycine receptor beta) is a neurotransmitter-gated ion channel concentrated within the spinal cord and brainstem. Expression is also observed in several upper brain regions including the cortex, cerebellum, hippocampus and amygdala. Binding of glycine to GLRB increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing), controlling spinal reflexes and locomotor behavior.

Sequence:

P47871 GLR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPPCQPQRPLLLLLLLLACQPQVPSAQVMDFLFEKWKLYGDQCHHNLSLLPPPTELVCNRTFDKYSCWPDTPANTTANISCPWYLPWHHKVQHRFVFKRCGPDGQWVRGPRGQPWRDASQCQMDGEEIEVQKEVAKMYSSFQVMYTVGYSLSLGALLLALAILGGLSKLHCTRNAIHANLFASFVLKASSVLVIDGLLRTRYSQKIGDDLSVSTWLSDGAVAGCRVAAVFMQYGIVANYCWLLVEGLYLHNLLGLATLPERSFFSLYLGIGWGAPMLFVVPWAVVKCLFENVQCWTSNDNMGFWWILRFPVFLAILINFFIFVRIVQLLVAKLRARQMHHTDYKFRLAKSTLTLIPLLGVHEVVFAFVTDEHAQGTLRSAKLFFDLFLSSFQGLLVAVLYCFLNKEVQSELRRRWHRWRLGKVLWEERNTSNHRASSSPGHGPPSKELQFGRGGGSQDSSAETPLAGGLPRLAESPF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Dog

Sheep

Pig

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P47871 As Substrate

P47871

Site	PTM Type	Source
S25	Phosphorylation	Uniprot
N46	N-Glycosylation	Uniprot
N59	N-Glycosylation	Uniprot
N74	N-Glycosylation	Uniprot
N78	N-Glycosylation	Uniprot
S437	Phosphorylation	Uniprot
S438	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
S456	Phosphorylation	Uniprot
S459	Phosphorylation	Uniprot
S460	Phosphorylation	Uniprot
T463	Phosphorylation	Uniprot

Research Backgrounds

P47871_GLR_HUMAN

Function:

G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.

PTMs:

Ligand-binding promotes phosphorylation of serine residues in the C-terminal cytoplasmic domain. Phosphorylation is important for receptor endocytosis after ligand-binding.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Is rapidly internalized after ligand-binding.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GLR Antibody - #AF5319