Product: MKL1 Antibody
Catalog: AF5302
Description: Rabbit polyclonal antibody to MKL1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Chicken
Mol.Wt.: 99 kDa; 99kD(Calculated).
Uniprot: Q969V6
RRID: AB_2837787

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Chicken(100%)
Clonality:
Polyclonal
Specificity:
MKL1 Antibody detects endogenous levels of total MKL1.
RRID:
AB_2837787
Cite Format: Affinity Biosciences Cat# AF5302, RRID:AB_2837787.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AI852829; AMKL; AW743281; AW821984; Basic SAP coiled-coil transcription activator; Bsac; KIAA1438; Mal; Megakaryoblastic leukemia 1 protein; Megakaryocytic acute leukemia protein; MKL (megakaryoblastic leukemia)/myocardin like 1; MKL/myocardin like protein 1; MKL/myocardin-like protein 1; Mkl1; MKL1_HUMAN; MRTF A; Mrtf A; MRTF-A; MRTFA; MYOCARDIN RELATED PROTEIN; Myocardin related transcription factor A; Myocardin-related transcription factor A;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q969V6 MRTFA_HUMAN:

Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.

Description:
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
Sequence:
MPPLKSPAAFHEQRRSLERARTEDYLKRKIRSRPERSELVRMHILEETSAEPSLQAKQLKLKRARLADDLNEKIAQRPGPMELVEKNILPVESSLKEAIIVGQVNYPKVADSSSFDEDSSDALSPEQPASHESQGSVPSPLEARVSEPLLSATSASPTQVVSQLPMGRDSREMLFLAEQPPLPPPPLLPPSLTNGTTIPTAKSTPTLIKQSQPKSASEKSQRSKKAKELKPKVKKLKYHQYIPPDQKQDRGAPPMDSSYAKILQQQQLFLQLQILNQQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTTNSSSSSGAPGPCGLARQNSTSLTGKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELIERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPSLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQMDDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWDSCL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969V6 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
S114 Phosphorylation
S124 Phosphorylation
S130 Phosphorylation
S139 Phosphorylation
S154 Phosphorylation
S156 Phosphorylation
T204 Phosphorylation
K237 Methylation
S295 Phosphorylation
S302 Phosphorylation
S303 Phosphorylation
T305 Phosphorylation
S310 Phosphorylation
S312 Phosphorylation P49137 (MAPKAPK2)
T313 Phosphorylation
S317 Phosphorylation
C326 S-Nitrosylation
S333 Phosphorylation P49137 (MAPKAPK2)
T334 Phosphorylation
S335 Phosphorylation
T337 Phosphorylation
K351 Methylation
S367 Phosphorylation
T369 Phosphorylation
Y380 Phosphorylation
S385 Phosphorylation
K392 Ubiquitination
S416 Phosphorylation
S446 Phosphorylation
T447 Phosphorylation
S449 Phosphorylation
T450 Phosphorylation
S454 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S458 Phosphorylation
S470 Phosphorylation
T471 Phosphorylation
T475 Phosphorylation
K499 Sumoylation
S507 Phosphorylation
S511 Phosphorylation
K532 Ubiquitination
K544 Ubiquitination
K552 Ubiquitination
T573 Phosphorylation P24941 (CDK2)
K576 Sumoylation
K624 Sumoylation
S695 Phosphorylation
S807 Phosphorylation

Research Backgrounds

Function:

Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus.

PTMs:

Phosphorylation at Ser-6 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Subcellular location is tightly regulated by actin both in cytoplasm and nucleus: high levels of G-actin in the nucleus observed during serum deprivation lead to low levels of nuclear MRTFA, while reduced levels of nuclear G-actin result in accumulation of MRTFA in the nucleus (By similarity). G-actin-binding in the cytoplasm inhibits nuclear import by masking the nuclear localization signal (NLS) (By similarity). In contrast, binding to nuclear globular actin (G-actin) promotes nuclear export to the cytoplasm (By similarity). Nuclear localization is regulated by MICAL2, which mediates depolymerization of nuclear actin, which decreases nuclear G-actin pool, thereby promoting retention of MRTFA in the nucleus and subsequent formation of an active complex with SRF (PubMed:24440334).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.

Subunit Structure:

Interacts with SRF, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF. Interacts (via RPEL repeats) with globular actin (G-actin), thereby regulating its subcellular location and activity of the complex formed with SRF. Either forms a trivalent (by binding three G-actin monomers) or pentavalent (by binding five G-actin monomers) complex with G-actin (By similarity). Forms a nuclear ternary complex with SCAI and SRF, leading to suppress MRTFA-induced SRF transcriptional activity. Interacts with beta-actin (ACTB); interaction with ACTB prevents interaction with SCAI (By similarity). Interacts with MRTFB.

Family&Domains:

The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity.

The RPEL repeats mediate binding to globular actin (G-actin); each RPEL repeat-binding to one G-actin monomer. In addition, each intervening spacer sequence region can bind one G-actin monomer, to reach a pentavalent complex.

Restrictive clause

 

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