MGMT Antibody | Affinity Biosciences

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Western blot analysis of MGMT expression in Jurkat cells.

Product:	MGMT Antibody
Catalog:	AF5295
Description:	Rabbit polyclonal antibody to MGMT
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	22 kDa; 22kD(Calculated).
Uniprot:	P16455
RRID:	AB_2837780

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

MGMT Antibody detects endogenous levels of total MGMT.

RRID:

AB_2837780
Cite Format: Affinity Biosciences Cat# AF5295, RRID:AB_2837780.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT; MGMT_HUMAN; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase;

Immunogens

Immunogen:

Uniprot:

P16455(MGMT_HUMAN) >>Visit HPA database.

Gene(ID):

MGMT(4255)

Description:

MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction.

Sequence:

P16455 MGMT_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGEVISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHRLGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN

PTMs - P16455 As Substrate

P16455

Site	PTM Type	Source
K8	Ubiquitination	Uniprot
T10	Phosphorylation	Uniprot
T11	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
K18	Ubiquitination	Uniprot
S22	Phosphorylation	Uniprot
K32	Ubiquitination	Uniprot
K101	Ubiquitination	Uniprot
K104	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
Y158	Phosphorylation	Uniprot
K165	Acetylation	Uniprot
K165	Ubiquitination	Uniprot
K178	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot
S201	Phosphorylation	Uniprot

Research Backgrounds

P16455_MGMT_HUMAN

Function:

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the MGMT family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MGMT Antibody - #AF5295