Kir6.2 Antibody - #AF5289

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Product: Kir6.2 Antibody
Catalog: AF5289
Description: Rabbit polyclonal antibody to Kir6.2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit
Mol.Wt.: 44 kDa, 55 kDa; 44kD(Calculated).
Uniprot: Q14654
RRID: AB_2837774

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Kir6.2 Antibody detects endogenous levels of total Kir6.2.
RRID:
AB_2837774
Cite Format: Affinity Biosciences Cat# AF5289, RRID:AB_2837774.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP sensitive inward rectifier potassium channel 11; Beta cell inward rectifier subunit; BIR; HHF 2; HHF2; IKATP; Inward rectifier K(+) channel Kir6.2; Inwardly rectifying potassium channel KIR6.2; IRK 11; IRK11; KCNJ11; Kir 6.2; Kir6.2; MGC133230; PHHI; Potassium channel inwardly rectifing subfamily J member 11; Potassium channel, inwardly rectifying subfamily J member 11; Potassium inwardly rectifying channel J11; TNDM 3; TNDM3;

Immunogens

Immunogen:
Uniprot:

Q14654(KCJ11_HUMAN) >>Visit HPA database.

Gene(ID):
KCNJ11(3767)
Description:
ATP-sensitive potassium (K(ATP)) channels are found in endocrine cells, neurons and both smooth and striated muscle, where they play an important role in controlling insulin secretion and vascular tone, and protect neurons under metabolic stress.
Sequence:
MLSRKGIIPEEYVLTRLAEDPAEPRYRARQRRARFVSKKGNCNVAHKNIREQGRFLQDVFTTLVDLKWPHTLLIFTMSFLCSWLLFAMAWWLIAFAHGDLAPSEGTAEPCVTSIHSFSSAFLFSIEVQVTIGFGGRMVTEECPLAILILIVQNIVGLMINAIMLGCIFMKTAQAHRRAETLIFSKHAVIALRHGRLCFMLRVGDLRKSMIISATIHMQVVRKTTSPEGEVVPLHQVDIPMENGVGGNSIFLVAPLIIYHVIDANSPLYDLAPSDLHHHQDLEIIVILEGVVETTGITTQARTSYLADEILWGQRFVPIVAEEDGRYSVDYSKFGNTIKVPTPLCTARQLDEDHSLLEALTLASARGPLRKRSVPMAKAKPKFSISPDSLS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14654 As Substrate

Site PTM Type Enzyme
T180 Phosphorylation Q9UQM7 (CAMK2A)
S208 Phosphorylation
T224 Phosphorylation Q9UQM7 (CAMK2A) , Q13557 (CAMK2D)
K332 Ubiquitination
T341 Phosphorylation P28482 (MAPK1)
S372 Phosphorylation
S385 Phosphorylation P28482 (MAPK1)

Research Backgrounds

Function:

This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.

PTMs:

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ABCC8/SUR. Interacts with ABCC9/SUR2.

Family&Domains:

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

References

1). Glyburide confers neuroprotection against age-related macular degeneration (AMD). Translational research : the journal of laboratory and clinical medicine, 2024 (PubMed: 38815899) [IF=7.8]

Application: IF/ICC    Species: Human    Sample: WERI-Rb1 cells

Fig. 2WERI-Rb1 human cone cells express glyburide target SUR1 and its potential partners A - Genes were detected by quantitative reverse transcription PCR (RT-qPCR) in WERI-Rb1 cells. Results were presented as the average of ∆Ct (delta cycle threshold) (∆=GAPDH Ct-studied gene Ct). SUR1(ABCC8), KIR6.2 and TRPM4 genes were expressed by WERI-Rb1 cells (n = 3 wells). B - SUR1, KIR6.2 and TRPM4 immunolabeling in WERI-Rb1 cells. SUR1 co-localized with KIR6.2 and possibly with TRPM4. Bar = 100 μm.

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