GRP94 Antibody - #AF5287

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Product: GRP94 Antibody
Catalog: AF5287
Description: Rabbit polyclonal antibody to GRP94
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 92 kDa; 92kD(Calculated).
Uniprot: P14625
RRID: AB_2837773

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(82%), Horse(82%), Sheep(82%), Rabbit(88%), Dog(82%)
Clonality:
Polyclonal
Specificity:
GRP94 Antibody detects endogenous levels of total GRP94.
RRID:
AB_2837773
Cite Format: Affinity Biosciences Cat# AF5287, RRID:AB_2837773.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

Immunogens

Immunogen:
Uniprot:

P14625(ENPL_HUMAN) >>Visit HPA database.

Gene(ID):
HSP90B1(7184)
Description:
Molecular chaperone that functions in the processing and transport of secreted proteins. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity.
Sequence:
MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Rabbit
88
Horse
82
Bovine
82
Sheep
82
Dog
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14625 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
T44 Phosphorylation
N62 N-Glycosylation
S64 Phosphorylation
K72 Ubiquitination
K75 Acetylation
K75 Ubiquitination
S92 Phosphorylation
Y94 Phosphorylation
K95 Ubiquitination
K97 Acetylation
K97 Ubiquitination
S106 Phosphorylation
N107 N-Glycosylation
S109 Phosphorylation
K114 Acetylation
K114 Ubiquitination
S127 Phosphorylation
T133 Phosphorylation
K135 Ubiquitination
K140 Acetylation
K142 Ubiquitination
T148 Phosphorylation
T150 Phosphorylation
K161 Ubiquitination
T165 Phosphorylation
K168 Ubiquitination
S169 Phosphorylation
T171 Phosphorylation
S172 Phosphorylation
K177 Methylation
T179 Phosphorylation
S187 Phosphorylation
Y200 Phosphorylation
S201 Phosphorylation
N217 N-Glycosylation
S225 Phosphorylation
S227 Phosphorylation
K252 Ubiquitination
S256 Phosphorylation
Y258 Phosphorylation
K265 Ubiquitination
K270 Ubiquitination
Y280 Phosphorylation
T288 Phosphorylation
S306 Phosphorylation
K340 Ubiquitination
S347 Phosphorylation
K348 Ubiquitination
K356 Ubiquitination
K360 Ubiquitination
S385 Phosphorylation
T391 Phosphorylation
S392 Phosphorylation
R395 Methylation
Y401 Phosphorylation
S403 Phosphorylation
K404 Acetylation
K404 Ubiquitination
K405 Ubiquitination
Y408 Phosphorylation
K410 Ubiquitination
Y429 Phosphorylation
K434 Ubiquitination
S439 Phosphorylation
N445 N-Glycosylation
S447 Phosphorylation
K455 Ubiquitination
K458 Ubiquitination
K467 Ubiquitination
T468 Phosphorylation
K473 Ubiquitination
K479 Acetylation
K479 Ubiquitination
K486 Methylation
K486 Ubiquitination
K493 Ubiquitination
S501 Phosphorylation
T504 Phosphorylation
R511 Methylation
S515 Phosphorylation
T519 Phosphorylation
Y527 Phosphorylation
S544 Phosphorylation
S545 Phosphorylation
S551 Phosphorylation
S552 Phosphorylation
K561 Ubiquitination
K586 Acetylation
K586 Sumoylation
K586 Ubiquitination
K593 Ubiquitination
T604 Phosphorylation
K613 Acetylation
K613 Ubiquitination
K623 Ubiquitination
K633 Ubiquitination
T641 Phosphorylation
S643 Phosphorylation
Y652 Phosphorylation
K663 Methylation
K663 Ubiquitination
Y667 Phosphorylation
T669 Phosphorylation
K671 Ubiquitination
S674 Phosphorylation
T675 Phosphorylation
Y677 Phosphorylation
Y678 Phosphorylation
S680 Phosphorylation
K682 Acetylation
K682 Ubiquitination
K683 Ubiquitination
T684 Phosphorylation
R690 Methylation
Y727 Phosphorylation
K733 Ubiquitination
Y735 Phosphorylation
R738 Methylation
R744 Methylation
S746 Phosphorylation
T774 Phosphorylation
T786 Phosphorylation
T792 Phosphorylation

Research Backgrounds

Function:

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).

Subcellular Location:

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with METTL23.

Family&Domains:

Belongs to the heat shock protein 90 family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

References

1). Structure‒function‒pathogenicity analysis of C-terminal myocilin missense variants based on experiments and 3D models. Frontiers in Genetics, 2022 (PubMed: 36267417) [IF=3.7]

Application: IF/ICC    Species: Human    Sample:

FIGURE 4 Nonsecreted MYOC mutants induce ER stress and impair autophagy. (A,B) Confocal double immunofluorescence for myocilin (red) and Grp94 (green) in iHTMCs transiently expressing WT or mutated MYOC. Nonsecreted mutations, including S425P and D380Y, show considerable colocalization of myocilin with Grp94. Scale bar: 25 μm. (C,D) iHTMCs expressing WT or mutant myocilins were immunolabeled with LC3 (green) and MYOC (red). S425P and D380Y mutants present increased intracellular myocilin and LC3, with no colocalization of these two proteins. Scale bar: 25 μm.

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