PSD95 Antibody - #AF5283

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Product: PSD95 Antibody
Catalog: AF5283
Description: Rabbit polyclonal antibody to PSD95
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 105 kDa; 80kD(Calculated).
Uniprot: P78352
RRID: AB_2827690

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
PSD95 Antibody detects endogenous levels of total PSD95.
RRID:
AB_2827690
Cite Format: Affinity Biosciences Cat# AF5283, RRID:AB_2827690.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Discs large homolog 4; Disks large homolog 4; DLG 4; Dlg4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15;

Immunogens

Immunogen:

A synthesized peptide derived from human PSD95, corresponding to a region within C-terminal amino acids.

Uniprot:

P78352(DLG4_HUMAN) >>Visit HPA database.

Gene(ID):
DLG4(1742)
Expression:
P78352 DLG4_HUMAN:

Brain.

Description:
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.
Sequence:
MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
80
Pig
0
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression.

PTMs:

Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses (By similarity). Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering (By similarity). Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons (By similarity).

Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Cytoplasm. Cell projection>Axon. Cell projection>Dendritic spine. Cell projection>Dendrite. Cell junction>Synapse>Presynapse.
Note: High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain.

Family&Domains:

The PDZ domain 3 mediates interaction with ADR1B.

The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

Belongs to the MAGUK family.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Organismal Systems > Nervous system > Glutamatergic synapse.

References

1). The brain-protective mechanism of fecal microbiota transplantation from young donor mice in the natural aging process via exosome, gut microbiota, and metabolomics analyses. Pharmacological research, 2024 (PubMed: 39053865) [IF=9.1]
2). SiNiSan ameliorates depression-like behavior in rats by enhancing synaptic plasticity via the CaSR-PKC-ERK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 31958763) [IF=6.9]

Application: WB    Species: rat    Sample: HIP

Fig. 5. |Effects of SNS on synaptic-associated protein in the HIP and PFC of stressed rats. Representative immunoblots for PSD-95, GAP-43, Syn, and Tublin in the HIP(A) and PFC(B) regions. (A) Results of relative protein levels of PSD-95, GAP-43, and Syn in the HIP of rats of each group.
3). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.2]
4). Sigma-1 Receptor as a Protective Factor for Diabetes-Associated Cognitive Dysfunction via Regulating Astrocytic Endoplasmic Reticulum-Mitochondrion Contact and Endoplasmic Reticulum Stress. Cells, 2023 (PubMed: 36611988) [IF=6.0]

Application: WB    Species: Mouse    Sample:

Figure 3. The effect of Sig-1R agonist on synapse change in the hippocampus of mice with T1DM. (a–c) Representative Western blots and quantitation of PSD-95 and Syp protein expression in CON, CON + PRE-084, STZ, and STZ + PRE-084 mice (n = 6). (d–g) Representative images of synaptic ultrastructure (d), quantitative analysis of PSD length (e) and width (f), and synaptic cleft (g) (n = 85–90; 3 animals per group). The scale bars are 500 and 200 nm. (h) Golgi staining images of the neuronal morphology. The scale bar is 50 μm. (i) Representative three-dimensional reconstruction images of different types of the spine in hippocampal neurons, including stubby (red), mushroom (green), thin (blue), and filopodia (purple) spines. The scale bar is 2 μm. (j–n) Quantitation of density of different spines, including total (j), stubby (k), mushroom (l), thin (m), and filopodia (n) spines (n = 20; 3 mice per group). The data were presented as mean ± SEM and analyzed via one-way ANOVA with Tukey’s multiple comparison tests, Welch’s ANOVA test followed by Dunnett’s T3 multiple comparison tests, and Kruskal-Wallis test followed by Dunn’s multiple comparison tests. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Sig-1R, sigma-1 receptor; T1DM, type 1 diabetes mellitus; PSD, postsynaptic density; Syp, synaptophysin; CON, control mice; STZ, mice with T1DM.
5). Exosomes derived from human induced pluripotent stem cell-derived neural progenitor cells protect neuronal function under ischemic conditions. Neural Regeneration Research, 2021 (PubMed: 33642395) [IF=5.9]

Application: WB    Species: Rat    Sample: neural progenitor cells

Figure 3 Effect of iPSC-NPC-derived exosomes (OGD+iNPC-exo) on expression of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins in OGD induced neurons. (A–C) mRNA expression (A) and protein expression (B, C) of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins (NF200, GAP-43, Synapsin, and PSD95) analyzed by polymerase chain reaction and western blot assay. The mRNA expression is described by the optical density ratio relative to the control group. Protein expression was described by the optical density ratio relative to β-actin. Data are presented as mean ± SD. ***P < 0.001, vs. control group; ###P < 0.001 (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times. GAP43: growth associated protein 43; iPSC-NPCs: induced pluripotent stem cells-derived neural progenitor cells; NF200: neurofilament 200; OGD: oxygen-glucose deprivation; p-Akt: phosphor-Akt; PSD95: postsynaptic density protein 95; PTEN: phosphatase and tensin homolog deleted on chromosome ten.
6). Research on the anti-aging mechanisms of Panax ginseng extract in mice: a gut microbiome and metabolomics approach. Frontiers in pharmacology, 2024 (PubMed: 38966558) [IF=5.6]
7). Early-Life Stress Induces Depression-Like Behavior and Synaptic-Plasticity Changes in a Maternal Separation Rat Model: Gender Difference and Metabolomics Study. Frontiers in Pharmacology, 2020 (PubMed: 32174832) [IF=5.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 MS reduces the expression of synaptic-plasticity protein. (A) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in the hippocampus by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. (B) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in cortex by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. Statistical analyses are performed by two-way ANOVA followed by t-test. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, n = 3 per group.
8). Gandouling induces GSK3β promoter methylation to improve cognitive impairment in Wilson's disease. Journal of ethnopharmacology, 2024 (PubMed: 38925320) [IF=4.8]
9). Gandouling induces GSK3β promoter methylation to improve cognitive impairment in Wilson's disease. Journal of ethnopharmacology, 2024 (PubMed: 38925320) [IF=4.8]
10). Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn. NEUROPHARMACOLOGY, 2019 (PubMed: 30721695) [IF=4.6]
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