PSD95 Antibody - #AF5283

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Product: PSD95 Antibody
Catalog: AF5283
Description: Rabbit polyclonal antibody to PSD95
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 105 kDa; 80kD(Calculated).
Uniprot: P78352
RRID: AB_2827690

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
PSD95 Antibody detects endogenous levels of total PSD95.
RRID:
AB_2827690
Cite Format: Affinity Biosciences Cat# AF5283, RRID:AB_2827690.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Discs large homolog 4; Disks large homolog 4; DLG 4; Dlg4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15;

Immunogens

Immunogen:

A synthesized peptide derived from human PSD95, corresponding to a region within C-terminal amino acids.

Uniprot:

P78352(DLG4_HUMAN) >>Visit HPA database.

Gene(ID):
DLG4(1742)
Expression:
P78352 DLG4_HUMAN:

Brain.

Description:
Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.
Sequence:
MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDASDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
80
Pig
0
Dog
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression.

PTMs:

Palmitoylated. Palmitoylation is required for targeting to postsynaptic density, plasma membrane and synapses (By similarity). Palmitoylation may play a role in glutamate receptor GRIA1 synapse clustering (By similarity). Depalmitoylated by ABHD17A and ABHD17B and to a lesser extent by ABHD17C, ABHD12, ABHD13, LYPLA1 and LYPLA2. Undergoes rapid synaptic palmitoylation/depalmitoylation cycles during neuronal development which slow down in mature neurons (By similarity).

Ubiquitinated by MDM2 in response to NMDA receptor activation, leading to proteasome-mediated degradation of DLG4 which is required for AMPA receptor endocytosis.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Cell junction>Synapse>Postsynaptic density. Cell junction>Synapse. Cytoplasm. Cell projection>Axon. Cell projection>Dendritic spine. Cell projection>Dendrite. Cell junction>Synapse>Presynapse.
Note: High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain.

Family&Domains:

The PDZ domain 3 mediates interaction with ADR1B.

The L27 domain near the N-terminus of isoform 2 is required for HGS/HRS-dependent targeting to postsynaptic density.

Belongs to the MAGUK family.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Substance dependence > Cocaine addiction.

· Organismal Systems > Nervous system > Glutamatergic synapse.

References

1). SiNiSan ameliorates depression-like behavior in rats by enhancing synaptic plasticity via the CaSR-PKC-ERK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2020 (PubMed: 31958763) [IF=6.9]

Application: WB    Species: rat    Sample: HIP

Fig. 5. |Effects of SNS on synaptic-associated protein in the HIP and PFC of stressed rats. Representative immunoblots for PSD-95, GAP-43, Syn, and Tublin in the HIP(A) and PFC(B) regions. (A) Results of relative protein levels of PSD-95, GAP-43, and Syn in the HIP of rats of each group.
2). Cyfluthrin exposure during pregnancy causes neurotoxicity in offspring-Ca2+ overload via IP3R-GRP75-VDAC1 pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 38492481) [IF=6.2]
3). Exosomes derived from human induced pluripotent stem cell-derived neural progenitor cells protect neuronal function under ischemic conditions. Neural Regeneration Research, 2021 (PubMed: 33642395) [IF=5.9]

Application: WB    Species: Rat    Sample: neural progenitor cells

Figure 3 Effect of iPSC-NPC-derived exosomes (OGD+iNPC-exo) on expression of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins in OGD induced neurons. (A–C) mRNA expression (A) and protein expression (B, C) of the PTEN/AKT signaling pathway and of synaptic plasticity-related proteins (NF200, GAP-43, Synapsin, and PSD95) analyzed by polymerase chain reaction and western blot assay. The mRNA expression is described by the optical density ratio relative to the control group. Protein expression was described by the optical density ratio relative to β-actin. Data are presented as mean ± SD. ***P < 0.001, vs. control group; ###P < 0.001 (one-way analysis of variance with post hoc Bonferroni test). All experiments were repeated three times. GAP43: growth associated protein 43; iPSC-NPCs: induced pluripotent stem cells-derived neural progenitor cells; NF200: neurofilament 200; OGD: oxygen-glucose deprivation; p-Akt: phosphor-Akt; PSD95: postsynaptic density protein 95; PTEN: phosphatase and tensin homolog deleted on chromosome ten.
4). Early-Life Stress Induces Depression-Like Behavior and Synaptic-Plasticity Changes in a Maternal Separation Rat Model: Gender Difference and Metabolomics Study. Frontiers in Pharmacology, 2020 (PubMed: 32174832) [IF=5.6]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 MS reduces the expression of synaptic-plasticity protein. (A) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in the hippocampus by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. (B) The bands of synaptic-plasticity proteins of SYN, PSD-95, and GAP-43 in cortex by WB. Statistical results indicate the relative protein levels expressed by SYN, GAP-43, and PSD-95. Statistical analyses are performed by two-way ANOVA followed by t-test. Data are presented as mean ± SEM, *p < 0.05, **p < 0.01, n = 3 per group.
5). Gandouling induces GSK3β promoter methylation to improve cognitive impairment in Wilson’s disease. JOURNAL OF ETHNOPHARMACOLOGY, 2024 [IF=4.8]
6). Ubiquitination and inhibition of glycine receptor by HUWE1 in spinal cord dorsal horn. NEUROPHARMACOLOGY, 2019 (PubMed: 30721695) [IF=4.6]
7). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB    Species: mouse    Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (A) CHIP knock-down protein was designed by siRNA in N2a cells. Approximately 5 µl Stub1-siRNA was able to achieve effective knock-down CHIP expression, and consequently the expression level of synapsin I S9 was decreased with no change in synaptic protein SNAP25 and PSD95 expression.
8). Effect of remimazolam toluene sulfonate on the cognitive function of juveniles and its mechanism of action. European journal of medical research, 2024 (PubMed: 39533344) [IF=4.2]
9). Adverse effects of iron deficiency anemia on pregnancy outcome and offspring development and intervention of three iron supplements. Scientific Reports, 2021 (PubMed: 33446747) [IF=3.8]

Application: WB    Species: Rat    Sample: hippocampus

Figure 5 Iron related indexes and neural development of offspring rats after iron supplement treatment. Western blot analysis for FTL and Tf in liver (a–c) and spleen (d–f). Morris water maze test for day 1 escape latency (g) and day 2 escape latency (h). Western blot analysis for FTL and Tf in brain (i–k) and hippocampus (l–n), and for SYN1, NMDAR, PSD-95 in hippocampus (o–r). The quantification of western blotting was provided in supplementary material. FTL ferritin light chain, Tf transferrin, SYN1 synapsin 1, NMDAR N-methyl-D-aspartate receptor, PSD-95 postsynaptic density protein 95. Data of Western blot analysis (mean ± SD) are expressed as the ratio of the relative contents between the value from IDA group and NG group and six iron treatment groups (n = 3). The relative contents of target proteins were quantified using the ratio between the optical density (OD) of target protein and the amount of the housekeeping protein GAPDH. **p < 0.01, compared with NG, #p < 0.05, ##p < 0.01, compared with IDAG. One-way ANOVA followed by Tukey multiple comparison test was used for comparison among 8 different groups.
10). Increased Fibronectin Impairs the Function of Excitatory/Inhibitory Synapses in Hirschsprung Disease. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2020 (PubMed: 31760535) [IF=3.6]

Application: IF/ICC    Species: rat    Sample: PC12 cell

Fig. 3|Suppression of FN increases the expression of synaptic proteins. A Relative expression of NL-1, PSD-95, vGLUT1, NL-2, SLC32, and FN mRNAs were signifcantly increased in the FN-KD group compared with the NC group and B Western blots of PC12 cell lysates in the FN-KD, NC, and Ctr groups with NL-1, PSD-95, NL-2, SLC32, and FN antibodies. The quantitative results (right) confrm that silencing FN signifcantly increases the expression of neuronal marker proteins.C The co-expression of NL-1, or NL-2, or PSD-95, or SLC32 with FN was examined by double immunofuorescence staining. The results support the idea that downregulation of FN enhances NL-1, PSD-95, NL-2, and SLC32 expression. Values are mean ± SEM, *P < 0.05, **P < 0.01, and ***P < 0.001

Application: IHC    Species: human    Sample: ganglionic, transitional, and aganglionic colonic segments

Fig. 2|Quantitative histological evaluations of ganglionic, transitional, and aganglionic colonic segments of HSCR patients. A1–3, a1–3 PSD-95 group, B1–3, b1–3 SLC32 group, C1–3, c1–3 FN group, d histological score, based on the intensity of positive staining (brown), and e area of positive staining shown as a percentage of total tissue area. Figures are representative of at least fve separate experiments. The diferences among the three groups were all statistically signifcant. Values are mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 (ganglionic vs. aganglionic, P < 0.0001)
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