FZD2 Antibody - #AF5282

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Product: FZD2 Antibody
Catalog: AF5282
Description: Rabbit polyclonal antibody to FZD2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 70~85kDa; 64kD(Calculated).
Uniprot: Q14332
RRID: AB_2837768

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
FZD2 Antibody detects endogenous levels of total FZD2.
RRID:
AB_2837768
Cite Format: Affinity Biosciences Cat# AF5282, RRID:AB_2837768.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Frizzled homolog 2; Frizzled-2; Frizzled2; Fz 2; Fz-2; Fz2; FZD 2; FZD2; FZD2_HUMAN; FzE 2; FzE2; hFz 2; hFz2; Mfz10;

Immunogens

Immunogen:
Uniprot:

Q14332(FZD2_HUMAN) >>Visit HPA database.

Gene(ID):
FZD2(2535)
Expression:
Q14332 FZD2_HUMAN:

Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.

Description:
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes.
Sequence:
MRPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRHGETTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
67
Chicken
67
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14332 As Substrate

Site PTM Type Enzyme
S32 O-Glycosylation
K127 Ubiquitination
T165 O-Glycosylation
T166 O-Glycosylation
T179 O-Glycosylation
T194 O-Glycosylation
K206 Ubiquitination
K214 Ubiquitination
K446 Methylation
T534 Phosphorylation
S541 Phosphorylation
S547 Phosphorylation
Y552 Phosphorylation

Research Backgrounds

Function:

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

PTMs:

Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Subcellular Location:

Membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. In the adult, mainly found in heart, placenta, skeletal muscle, lung, kidney, pancreas, prostate, testis, ovary and colon. In the fetus, expressed in brain, lung and kidney. Low levels in fetal liver.

Family&Domains:

Lys-Thr-X-X-X-Trp motif interacts with the PDZ domain of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway.

The FZ domain is involved in binding with Wnt ligands.

Belongs to the G-protein coupled receptor Fz/Smo family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Human Diseases > Cancers: Specific types > Gastric cancer.   (View pathway)

· Organismal Systems > Endocrine system > Melanogenesis.

References

1). Ginsenoside Rd promotes omentin secretion in adipose through TBK1-AMPK to improve mitochondrial biogenesis via WNT5A/Ca2+ pathways in heart failure. Redox biology, 2023 (PubMed: 36652744) [IF=11.4]

Application: WB    Species: Mouse    Sample:

Fig. 5 Adipose tissue-specific omentin overexpression inhibited WNT5A/Ca2+ signaling pathway and improved mitochondrial biogenesis to ameliorate myocardial injury in HF mice. (A) Serum omentin level of HF patients and healthy subjects. A total of 58 HF patients and 38 healthy subjects were enrolled. (B) The omentin serum content of adipose tissue-specific omentin overexpression by intravenous injection of AAV-omentin and negative control by intravenous injection of AAV-NC (n = 8). (C) The expression of omentin in the inguinal fat of mice with adipose tissue-specific omentin overexpression was detected by immunohistochemistry (n = 4). Scale bar = 200 μm. (D) Representative echocardiographs of mice with AAV-omentin overexpression and the statistical results of (E) LV EF, (F) LV FS, (G) stroke volume, (H) LVPW; d, (I) IVS; d, (J) RWT and (K) LV Mass were presented (n = 8). (L) Representative TTC staining images of heart tissues of mice with AAV-omentin overexpression (n = 6). (M) Representative images of H&E and Masson staining of heart tissues of mice with AAV-omentin overexpression (n = 3), scale bar = 250 μm. (N) The serum content of BNP in mice with AAV-omentin overexpression (n = 8). (O) The serum CK activity in mice with AVV-omentin overexpression (n = 8). (P) The protein expression level of WNT5A, Frizzled2 and p-CAMKII/CAMKII were determined by Western blot. β-actin was used as a loading control (n = 5). (Q) The protein expression level of PGC-1α, NRF1, NRF2 and TFAM were determined by Western blot in mice with AAV-omentin overexpression. β-actin was used as a loading control (n = 5). Values are expressed as the means ± SD. (A) Unpaired Student's two-tailed t-test; (B–Q) One-way ANOVA followed by the Dunnett's post hoc test. #P < 0.05, ##P < 0.01, ###P < 0.001 vs. Sham; *P < 0.05, **P < 0.01, ***P < 0.001 vs. CAL.
2). 10‐Gingerol alleviates hypoxia/reoxygenation‐induced cardiomyocyte injury through inhibition of the Wnt5a/Frizzled‐2 pathway. Food Science & Nutrition, 2021 (PubMed: 34262748) [IF=3.9]

Application: WB    Species: Rat    Sample: H9c2 cells

FIGURE 10 Effects of 10- Gin on the Wnt5a (a, c, e) and Frizzled- 2 (b, d, f) gene and protein expressions. Data are represented as mean ± SEM. ** p <.01 versus. CONT; # p <.05, ## p <.01 versus. H/R group, n = 3
3). Miltirone suppresses ESCC cell viability and invasion by promoting E3 ligase mediated-FZD2 ubiquitination and degradation. Chemical Biology & Drug Design, 2024

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