hnRNP M Antibody | Affinity Biosciences

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Western blot analysis of hnRNP M expression in HT29 cells,The lane on the left was treated with the antigen-specific peptide.

AF5276 at 1/100 staining human kidney tissue sections by IHC-P.

Product:	hnRNP M Antibody
Catalog:	AF5276
Description:	Rabbit polyclonal antibody to hnRNP M
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	78 kDa; 78kD(Calculated).
Uniprot:	P52272
RRID:	AB_2837762

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

hnRNP M Antibody detects endogenous levels of total hnRNP M.

RRID:

AB_2837762
Cite Format: Affinity Biosciences Cat# AF5276, RRID:AB_2837762.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CEA receptor; CEAR; Heterogeneous nuclear ribonucleoprotein M; Heterogenous nuclear ribonucleoprotein M4; hnRNA binding protein M4; hnRNP M; Hnrnpm; HNRNPM4; HNRPM; HNRPM_HUMAN; HNRPM4; HTGR1; M3; M4; M4 protein; N-acetylglucosamine receptor 1; NAGR1;

Immunogens

Immunogen:

Uniprot:

P52272(HNRPM_HUMAN) >>Visit HPA database.

Gene(ID):

HNRNPM(4670)

Description:

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

Sequence:

P52272 HNRPM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAGVEAAAEVAATEIKMEEESGAPGVPSGNGAPGPKGEGERPAQNEKRKEKNIKRGGNRFEPYANPTKRYRAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRPLKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMITIPPSILNNPNIPNEIIHALQAGRLGSTVFVANLDYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGTVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDERALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLNKGIGMGNIGPAGMGMEGIGFGINKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGGGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDRMGSVERMGSGIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMGFGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMERMVPAGMGAGLERMGPVMDRMATGLERMGANNLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGGGGASFDRAIEMERGNFGGSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFESPEVAERACRMMNGMKLSGREIDVRIDRNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52272 As Substrate

P52272

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T14	Phosphorylation		Uniprot
K17	Sumoylation		Uniprot
S22	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
K37	Sumoylation		Uniprot
K37	Ubiquitination		Uniprot
K48	Acetylation		Uniprot
K48	Ubiquitination		Uniprot
K52	Methylation		Uniprot
K55	Sumoylation		Uniprot
R56	Methylation		Uniprot
Y64	Phosphorylation		Uniprot
T68	Phosphorylation		Uniprot
K69	Acetylation		Uniprot
K69	Ubiquitination		Uniprot
T76	Phosphorylation		Uniprot
K83	Acetylation		Uniprot
K83	Sumoylation		Uniprot
K83	Ubiquitination		Uniprot
S86	Phosphorylation		Uniprot
K88	Sumoylation		Uniprot
K88	Ubiquitination		Uniprot
K92	Ubiquitination		Uniprot
K94	Ubiquitination		Uniprot
T99	Phosphorylation		Uniprot
Y100	Phosphorylation		Uniprot
K110	Acetylation		Uniprot
K110	Sumoylation		Uniprot
K110	Ubiquitination		Uniprot
C114	S-Nitrosylation		Uniprot
K120	Sumoylation		Uniprot
K120	Ubiquitination		Uniprot
K126	Sumoylation		Uniprot
K126	Ubiquitination		Uniprot
K127	Sumoylation		Uniprot
K127	Ubiquitination		Uniprot
K134	Acetylation		Uniprot
K134	Sumoylation		Uniprot
K134	Ubiquitination		Uniprot
S136	Phosphorylation		Uniprot
S138	Phosphorylation		Uniprot
K143	Sumoylation		Uniprot
K143	Ubiquitination		Uniprot
K145	Sumoylation		Uniprot
K145	Ubiquitination		Uniprot
S204	Phosphorylation		Uniprot
T205	Phosphorylation		Uniprot
Y213	Phosphorylation		Uniprot
K214	Acetylation		Uniprot
K214	Ubiquitination		Uniprot
K218	Ubiquitination		Uniprot
K221	Sumoylation		Uniprot
K221	Ubiquitination		Uniprot
K239	Acetylation		Uniprot
K239	Sumoylation		Uniprot
K239	Ubiquitination		Uniprot
K242	Sumoylation		Uniprot
K242	Ubiquitination		Uniprot
K277	Acetylation		Uniprot
K277	Sumoylation		Uniprot
K277	Ubiquitination		Uniprot
K285	Ubiquitination		Uniprot
K322	Sumoylation		Uniprot
K322	Ubiquitination		Uniprot
K345	Ubiquitination		Uniprot
S365	Phosphorylation		Uniprot
R371	Methylation		Uniprot
S377	Phosphorylation		Uniprot
K381	Acetylation		Uniprot
K381	Sumoylation		Uniprot
K381	Ubiquitination		Uniprot
K388	Sumoylation		Uniprot
K388	Ubiquitination		Uniprot
S397	Phosphorylation		Uniprot
R410	Methylation		Uniprot
R429	Methylation		Uniprot
S432	Phosphorylation	O14757 (CHEK1)	Uniprot
R443	Methylation		Uniprot
S446	Phosphorylation		Uniprot
S452	Phosphorylation		Uniprot
S467	Phosphorylation		Uniprot
S468	Phosphorylation		Uniprot
T475	Phosphorylation		Uniprot
S481	Phosphorylation		Uniprot
R485	Methylation		Uniprot
R496	Methylation		Uniprot
R503	Methylation		Uniprot
T507	Phosphorylation		Uniprot
S513	Phosphorylation		Uniprot
S528	Phosphorylation		Uniprot
R543	Methylation		Uniprot
R550	Methylation		Uniprot
R565	Methylation		Uniprot
S575	Phosphorylation		Uniprot
S588	Phosphorylation		Uniprot
S618	Phosphorylation		Uniprot
R621	Methylation		Uniprot
R627	Methylation		Uniprot
S633	Phosphorylation		Uniprot
S637	Phosphorylation		Uniprot
K651	Sumoylation		Uniprot
K651	Ubiquitination		Uniprot
T665	Phosphorylation		Uniprot
K667	Acetylation		Uniprot
K667	Sumoylation		Uniprot
K667	Ubiquitination		Uniprot
K670	Ubiquitination		Uniprot
K672	Acetylation		Uniprot
K672	Sumoylation		Uniprot
K672	Ubiquitination		Uniprot
C676	S-Nitrosylation		Uniprot
Y681	Phosphorylation		Uniprot
K685	Acetylation		Uniprot
K685	Sumoylation		Uniprot
K685	Ubiquitination		Uniprot
K690	Ubiquitination		Uniprot
K692	Ubiquitination		Uniprot
C694	S-Nitrosylation		Uniprot
K698	Acetylation		Uniprot
K698	Sumoylation		Uniprot
K698	Ubiquitination		Uniprot
S701	Phosphorylation		Uniprot
C709	S-Nitrosylation		Uniprot
K716	Sumoylation		Uniprot
K716	Ubiquitination		Uniprot

Research Backgrounds

P52272_HNRPM_HUMAN

Function:

PTMs:

Sumoylated.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Identified in the spliceosome C complex.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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For Research Use Only.
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hnRNP M Antibody - #AF5276