CYB5 Antibody - #AF5273

(3)  
Product: CYB5 Antibody
Catalog: AF5273
Description: Rabbit polyclonal antibody to CYB5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 15 kDa; 15kD(Calculated).
Uniprot: P00167
RRID: AB_2837759

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(80%), Horse(80%), Rabbit(82%), Dog(82%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
CYB5 Antibody detects endogenous levels of total CYB5.
RRID:
AB_2837759
Cite Format: Affinity Biosciences Cat# AF5273, RRID:AB_2837759.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYB 5; CYB 5A; CYB5; CYB5_HUMAN; Cyb5a; Cytochrome b 5; Cytochrome b5 (microsomal); Cytochrome b5; Cytochrome b5 type A (microsomal); Cytochrome b5 type A; MCB 5; MCB5; Microsomal cytochrome b5; Microsomal cytochrome b5 type A; Type 1 cyt b5;

Immunogens

Immunogen:
Uniprot:

P00167(CYB5_HUMAN) >>Visit HPA database.

Gene(ID):
CYB5A(1528)
Description:
Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]. A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia.
Sequence:
MAEQSDEAVKYYTLEEIQKHNHSKSTWLILHHKVYDLTKFLEEHPGGEEVLREQAGGDATENFEDVGHSTDAREMSKTFIIGELHPDDRPKLNKPPETLITTIDSSSSWWTNWVIPAISAVAVALMYRLYMAED

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Dog
82
Rabbit
82
Pig
80
Horse
80
Bovine
80
Xenopus
70
Zebrafish
70
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00167 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S5 Phosphorylation
K10 Ubiquitination
K19 Acetylation
K19 Ubiquitination
S25 Phosphorylation
Y35 Phosphorylation
K39 Acetylation
K39 Ubiquitination
R52 Methylation
T60 Phosphorylation
T70 Phosphorylation

Research Backgrounds

Function:

Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein>Cytoplasmic side. Microsome membrane>Single-pass membrane protein>Cytoplasmic side.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cytochrome b5 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.