hnRNP A1 Antibody - #AF5268
| Product: | hnRNP A1 Antibody |
| Catalog: | AF5268 |
| Description: | Rabbit polyclonal antibody to hnRNP A1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 39 kDa; 39kD(Calculated). |
| Uniprot: | P09651 |
| RRID: | AB_2837754 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5268, RRID:AB_2837754.
Fold/Unfold
HNRNPA 1; Helix destabilizing protein; Helix-destabilizing protein; Heterogeneous nuclear ribonucleoprotein A1; Heterogeneous nuclear ribonucleoprotein A1B protein; Heterogeneous nuclear ribonucleoprotein B2 protein; Heterogeneous nuclear ribonucleoprotein core protein A1; hnRNP A1; hnRNP core protein A1; HNRNPA1; HNRPA1; MGC102835; Nuclear ribonucleoprotein particle A1 protein; ROA1_HUMAN; Single strand DNA binding protein UP1; Single strand RNA binding protein; Single-strand RNA-binding protein;
Immunogens
- P09651 ROA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSKSESPKEPEQLRKLFIGGLSFETTDESLRSHFEQWGTLTDCVVMRDPNTKRSRGFGFVTYATVEEVDAAMNARPHKVDGRVVEPKRAVSREDSQRPGAHLTVKKIFVGGIKEDTEEHHLRDYFEQYGKIEVIEIMTDRGSGKKRGFAFVTFDDHDSVDKIVIQKYHTVNGHNCEVRKALSKQEMASASSSQRGRSGSGNFGGGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGYGGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDSYNNGGGGGFGGGSGSNFGGGGSYNDFGNYNNQSSNFGPMKGGNFGGRSSGPYGGGGQYFAKPRNQGGYGGSSSSSSYGSGRRF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P09651 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| S4 | Phosphorylation | Q9UBS0 (RPS6KB2) | Uniprot |
| S6 | Phosphorylation | Q9UBS0 (RPS6KB2) | Uniprot |
| K8 | Acetylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| R14 | Methylation | Uniprot | |
| K15 | Acetylation | Uniprot | |
| K15 | Sumoylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| R31 | Methylation | Uniprot | |
| C43 | S-Nitrosylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| R55 | Methylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K87 | Acetylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| T103 | Phosphorylation | Uniprot | |
| K105 | Acetylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K106 | Methylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K113 | Acetylation | Uniprot | |
| K113 | Sumoylation | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| R122 | Methylation | Uniprot | |
| Y124 | Phosphorylation | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| R140 | Methylation | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| K145 | Acetylation | Uniprot | |
| R146 | Methylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Methylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K166 | Acetylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| C175 | S-Nitrosylation | Uniprot | |
| K183 | Sumoylation | Uniprot | |
| K183 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| R194 | Methylation | Uniprot | |
| R196 | Methylation | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | P17612 (PRKACA) , P68400 (CSNK2A1) , P31749 (AKT1) | Uniprot |
| R206 | Methylation | Uniprot | |
| R218 | Methylation | Uniprot | |
| R225 | Methylation | Uniprot | |
| R232 | Methylation | Uniprot | |
| Y244 | Phosphorylation | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| R265 | Methylation | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| Y318 | Phosphorylation | Uniprot | |
| K329 | Sumoylation | Uniprot | |
| R336 | Methylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| K350 | Acetylation | Uniprot | |
| K350 | Methylation | Uniprot | |
| K350 | Sumoylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| R352 | Methylation | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S363 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S364 | Phosphorylation | Q9BUB5 (MKNK1) | Uniprot |
| S365 | Phosphorylation | Uniprot | |
| Y366 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| R370 | Methylation | Uniprot | |
| R371 | Methylation | Uniprot |
Research Backgrounds
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May bind to specific miRNA hairpins. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1.
(Microbial infection) May play a role in HCV RNA replication.
(Microbial infection) Cleavage by Enterovirus 71 protease 3C results in increased translation of apoptosis protease activating factor APAF1, leading to apoptosis.
Arg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated.
Nucleus. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes (PubMed:17289661).
Cytoplasm.
Note: (Microbial infection) In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner.
Identified in the spliceosome C complex. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with SEPT6. Interacts with C9orf72. Interacts with KHDRBS1. Interacts with UBQLN2.
(Microbial infection) Interacts with HCV NS5B and with the 5'-UTR and 3'-UTR of HCV RNA.
Research Fields
· Genetic Information Processing > Transcription > Spliceosome.
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