hnRNP L Antibody | Affinity Biosciences

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AF5260 at 1/100 staining human kidney tissue sections by IHC-P.

Product:	hnRNP L Antibody
Catalog:	AF5260
Description:	Rabbit polyclonal antibody to hnRNP L
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.:	60 kDa; 64kD(Calculated).
Uniprot:	P14866
RRID:	AB_2837746

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

hnRNP L Antibody detects endogenous levels of total hnRNP L.

RRID:

AB_2837746
Cite Format: Affinity Biosciences Cat# AF5260, RRID:AB_2837746.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

D830027H13Rik; FLJ35509; Heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; Hnrnpl; hnRPL; HNRPL_HUMAN; P/OKcl.14;

Immunogens

Immunogen:

Uniprot:

P14866(HNRPL_HUMAN) >>Visit HPA database.

Gene(ID):

HNRNPL(3191)

Description:

Splicing factor binding to exonic or intronic sites and acting as either an activator or repressor of exon inclusion. Exhibits a binding preference for CA-rich elements. Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and associated with most nascent transcripts. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter.

Sequence:

P14866 HNRPL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRRLLPRAEKRRRRLEQRQQPDEQRRRSGAMVKMAAAGGGGGGGRYYGGGSEGGRAPKRLKTDNAGDQHGGGGGGGGGAGAAGGGGGGENYDDPHKTPASPVVHIRGLIDGVVEADLVEALQEFGPISYVVVMPKKRQALVEFEDVLGACNAVNYAADNQIYIAGHPAFVNYSTSQKISRPGDSDDSRSVNSVLLFTILNPIYSITTDVLYTICNPCGPVQRIVIFRKNGVQAMVEFDSVQSAQRAKASLNGADIYSGCCTLKIEYAKPTRLNVFKNDQDTWDYTNPNLSGQGDPGSNPNKRQRQPPLLGDHPAEYGGPHGGYHSHYHDEGYGPPPPHYEGRRMGPPVGGHRRGPSRYGPQYGHPPPPPPPPEYGPHADSPVLMVYGLDQSKMNCDRVFNVFCLYGNVEKVKFMKSKPGAAMVEMADGYAVDRAITHLNNNFMFGQKLNVCVSKQPAIMPGQSYGLEDGSCSYKDFSESRNNRFSTPEQAAKNRIQHPSNVLHFFNAPLEVTEENFFEICDELGVKRPSSVKVFSGKSERSSSGLLEWESKSDALETLGFLNHYQMKNPNGPYPYTLKLCFSTAQHAS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Sheep

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14866 As Substrate

P14866

Site	PTM Type	Enzyme	Source
S29	Phosphorylation		Uniprot
K34	Acetylation		Uniprot
K34	Ubiquitination		Uniprot
R46	Methylation		Uniprot
Y47	Phosphorylation		Uniprot
Y48	Phosphorylation		Uniprot
S52	Phosphorylation		Uniprot
R56	Methylation		Uniprot
K59	Acetylation		Uniprot
K59	Sumoylation		Uniprot
K59	Ubiquitination		Uniprot
K62	Sumoylation		Uniprot
K62	Ubiquitination		Uniprot
T63	Phosphorylation		Uniprot
Y92	Phosphorylation		Uniprot
K97	Acetylation		Uniprot
K97	Ubiquitination		Uniprot
T98	Phosphorylation		Uniprot
S101	Phosphorylation		Uniprot
R107	Methylation		Uniprot
K136	Sumoylation		Uniprot
S185	Phosphorylation		Uniprot
S188	Phosphorylation		Uniprot
K229	Ubiquitination		Uniprot
K248	Ubiquitination		Uniprot
S250	Phosphorylation		Uniprot
S258	Phosphorylation		Uniprot
C260	S-Nitrosylation		Uniprot
C261	S-Nitrosylation		Uniprot
K264	Acetylation		Uniprot
K264	Ubiquitination		Uniprot
Y267	Phosphorylation		Uniprot
K269	Acetylation		Uniprot
K269	Ubiquitination		Uniprot
K277	Sumoylation		Uniprot
K277	Ubiquitination		Uniprot
T282	Phosphorylation		Uniprot
Y285	Phosphorylation		Uniprot
S291	Phosphorylation		Uniprot
S298	Phosphorylation		Uniprot
K302	Acetylation		Uniprot
K302	Sumoylation		Uniprot
K302	Ubiquitination		Uniprot
Y328	Phosphorylation		Uniprot
Y333	Phosphorylation		Uniprot
Y340	Phosphorylation		Uniprot
R344	Methylation		Uniprot
R354	Methylation		Uniprot
R358	Methylation		Uniprot
Y359	Phosphorylation		Uniprot
Y363	Phosphorylation		Uniprot
Y375	Phosphorylation		Uniprot
S381	Phosphorylation		Uniprot
R398	Methylation		Uniprot
K411	Ubiquitination		Uniprot
K413	Ubiquitination		Uniprot
K416	Ubiquitination		Uniprot
K418	Sumoylation		Uniprot
K418	Ubiquitination		Uniprot
Y430	Phosphorylation		Uniprot
R434	Methylation		Uniprot
T437	Phosphorylation		Uniprot
K448	Acetylation		Uniprot
C452	S-Nitrosylation		Uniprot
K455	Ubiquitination		Uniprot
K475	Acetylation		Uniprot
K475	Ubiquitination		Uniprot
T487	Phosphorylation		Uniprot
K493	Ubiquitination		Uniprot
K527	Ubiquitination		Uniprot
S530	Phosphorylation		Uniprot
S531	Phosphorylation		Uniprot
K533	Ubiquitination		Uniprot
S536	Phosphorylation		Uniprot
K538	Sumoylation		Uniprot
K538	Ubiquitination		Uniprot
S543	Phosphorylation		Uniprot
S544	Phosphorylation	Q16566 (CAMK4)	Uniprot
K552	Acetylation		Uniprot
K552	Sumoylation		Uniprot
K552	Ubiquitination		Uniprot
S553	Phosphorylation		Uniprot
Y565	Phosphorylation		Uniprot
K568	Sumoylation		Uniprot
K568	Ubiquitination		Uniprot
Y574	Phosphorylation		Uniprot
Y576	Phosphorylation		Uniprot
T577	Phosphorylation		Uniprot
K579	Ubiquitination		Uniprot
C581	S-Nitrosylation		Uniprot
S589	Phosphorylation		Uniprot

Research Backgrounds

P14866_HNRPL_HUMAN

Function:

PTMs:

Several isoelectric forms of the L protein are probably the results of post-translational modifications.

Phosphorylation at Ser-544 by CaMK4 enhances interaction with a CaMK4-responsive RNA element (CaRRE1), and prevents inclusion of the stress axis-regulated exon (STREX) of the KCNMA1 potassium channel transcripts upon membrane depolarization.

Subcellular Location:

Nucleus>Nucleoplasm. Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. These granules are not identical with P bodies or stress granules.

Subunit Structure:

Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with HNRNPLL. Interacts with APEX1; the interaction is DNA-dependent. Component of a complex with SETD2. Interacts with ELAVL1.

Family&Domains:

RRM domain 2 has moderate RNA-binding affinity. RRM domains 3 and 4 may facilitate RNA looping when binding to two appropriately separated binding sites within the same target pre-mRNA (PubMed:23782695).

References

1). Stealthy nanoparticles protect endothelial barrier from leakiness by resisting the absorption of VE-cadherin. Nanoscale, 2021 (PubMed: 34259298) [IF=6.7]

Application: WB Species: Human Sample: HMVEC

Fig. 4 (a) Schematic showing the protein pull down experimental setup. Immunoblotting (left panel) and its semi-quantitative analysis (right panel) of (b) VEC, SOD1, claudin-5 and α-tublin from whole cell lysate and pulled down by different NPs. (c) Y658 (p-VEC(Y658)), Y731 (p-VEC(Y731), VEC and α-tublin from the whole cell lysate with/without the Src kinase inhibitor, PP1.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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hnRNP L Antibody - #AF5260