Product: SLC16A3 Antibody
Catalog: AF5253
Description: Rabbit polyclonal antibody to SLC16A3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 47 kDa; 49kD(Calculated).
Uniprot: O15427
RRID: AB_2837739

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
SLC16A3 Antibody detects endogenous levels of total SLC16A3.
RRID:
AB_2837739
Cite Format: Affinity Biosciences Cat# AF5253, RRID:AB_2837739.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MCT 4; MCT3; MCT4; Monocarboxylate transporter 3; Monocarboxylate transporter 4; MOT4_HUMAN; SLC16A3; Solute carrier family 16 member 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15427 MOT4_HUMAN:

Highly expressed in skeletal muscle.

Description:
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily
Sequence:
MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDTAWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGVITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGFLILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVLGLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSFSMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVLLMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPEVAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Xenopus
82
Chicken
82
Zebrafish
64
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15427 As Substrate

Site PTM Type Enzyme
S35 Phosphorylation
Y36 Phosphorylation
K254 Sumoylation
K262 Sumoylation
K415 Ubiquitination
K428 Ubiquitination
K431 Ubiquitination
S436 Phosphorylation
K448 Sumoylation
K448 Ubiquitination
K453 Ubiquitination
T460 Phosphorylation
T463 Phosphorylation
S464 Phosphorylation

Research Backgrounds

Function:

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in skeletal muscle.

Family&Domains:

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer.   (View pathway)

References

1). Metformin alleviates nickel-refining fumes-induced aerobic glycolysis via AMPK/GOLPH3 pathway in vitro and in vivo. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35405526) [IF=6.8]

Application: WB    Species: human    Sample: Beas-2B cells

Fig. 3. |Effects of Ni-refining fumes on the expression of aerobic glycolysis and mTOR signaling. *p < 0.05 vs. control group, **p < 0.01 vs. control group.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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