SLC16A3 Antibody | Affinity Biosciences

WB
IF/ICC
Cites

1/3

Western blot analysis of extracts from Hybridoma cells, using MOT4 Antibody.

Product:	SLC16A3 Antibody
Catalog:	AF5253
Description:	Rabbit polyclonal antibody to SLC16A3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	47 kDa; 49kD(Calculated).
Uniprot:	O15427
RRID:	AB_2837739

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Chicken(82%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

SLC16A3 Antibody detects endogenous levels of total SLC16A3.

RRID:

AB_2837739
Cite Format: Affinity Biosciences Cat# AF5253, RRID:AB_2837739.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

MCT 4; MCT3; MCT4; Monocarboxylate transporter 3; Monocarboxylate transporter 4; MOT4_HUMAN; SLC16A3; Solute carrier family 16 member 3;

Immunogens

Immunogen:

Uniprot:

O15427(MOT4_HUMAN) >>Visit HPA database.

Gene(ID):

SLC16A3(9123)

Expression:

O15427 MOT4_HUMAN:

Highly expressed in skeletal muscle.

Description:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily

Sequence:

O15427 MOT4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGGAVVDEGPTGVKAPDGGWGWAVLFGCFVITGFSYAFPKAVSVFFKELIQEFGIGYSDTAWISSILLAMLYGTGPLCSVCVNRFGCRPVMLVGGLFASLGMVAASFCRSIIQVYLTTGVITGLGLALNFQPSLIMLNRYFSKRRPMANGLAAAGSPVFLCALSPLGQLLQDRYGWRGGFLILGGLLLNCCVCAALMRPLVVTAQPGSGPPRPSRRLLDLSVFRDRGFVLYAVAASVMVLGLFVPPVFVVSYAKDLGVPDTKAAFLLTILGFIDIFARPAAGFVAGLGKVRPYSVYLFSFSMFFNGLADLAGSTAGDYGGLVVFCIFFGISYGMVGALQFEVLMAIVGTHKFSSAIGLVLLMEAVAVLVGPPSGGKLLDATHVYMYVFILAGAEVLTSSLILLLGNFFCIRKKPKEPQPEVAAAEEEKLHKPPADSGVDLREVEHFLKAEPEKNGEVVHTPETSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15427 As Substrate

O15427

Site	PTM Type	Source
S35	Phosphorylation	Uniprot
Y36	Phosphorylation	Uniprot
K254	Sumoylation	Uniprot
K262	Sumoylation	Uniprot
K415	Ubiquitination	Uniprot
K428	Ubiquitination	Uniprot
K431	Ubiquitination	Uniprot
S436	Phosphorylation	Uniprot
K448	Sumoylation	Uniprot
K448	Ubiquitination	Uniprot
K453	Ubiquitination	Uniprot
T460	Phosphorylation	Uniprot
T463	Phosphorylation	Uniprot
S464	Phosphorylation	Uniprot

Research Backgrounds

O15427_MOT4_HUMAN

Function:

Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Highly expressed in skeletal muscle.

Family&Domains:

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

References

1). Metformin alleviates nickel-refining fumes-induced aerobic glycolysis via AMPK/GOLPH3 pathway in vitro and in vivo. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2022 (PubMed: 35405526) [IF=6.8]

Application: WB Species: human Sample: Beas-2B cells

Fig. 3. |Effects of Ni-refining fumes on the expression of aerobic glycolysis and mTOR signaling. *p < 0.05 vs. control group, **p < 0.01 vs. control group.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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SLC16A3 Antibody - #AF5253