BUB3 Antibody - #AF5252
| Product: | BUB3 Antibody |
| Catalog: | AF5252 |
| Description: | Rabbit polyclonal antibody to BUB3 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 37 kDa; 37kD(Calculated). |
| Uniprot: | O43684 |
| RRID: | AB_2837738 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5252, RRID:AB_2837738.
Fold/Unfold
Aa2-050; AU019800; AU021329; AU043350; AW146323; BUB 3; BUB 3L; BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; bub3; BUB3 budding uninhibited by benzimidazoles 3 homolog; BUB3 mitotic checkpoint protein; BUB3_HUMAN; BUB3L; Budding uninhibited by benomyl; Budding uninhibited by benzimidazoles 3; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae); Budding uninhibited by benzimidazoles 3 homolog (yeast); Budding uninhibited by benzimidazoles 3 homolog; Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of; C78067; hBUB3; Mitotic checkpoint component; Mitotic checkpoint protein bub3; OTTHUMP00000020679; OTTHUMP00000020680; WD repeat type I transmembrane protein A72.5;
Immunogens
- O43684 BUB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43684 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Sumoylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | PR:O43683 (hBUB1) | Uniprot |
| S19 | Phosphorylation | O43683 (BUB1) | Uniprot |
| K21 | Ubiquitination | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| R126 | Methylation | Uniprot | |
| T127 | Phosphorylation | Uniprot | |
| C129 | S-Nitrosylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| R149 | Methylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| R186 | Methylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| Y207 | Phosphorylation | P14618 (PKM) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| K227 | Sumoylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K322 | Acetylation | Uniprot | |
| K322 | Sumoylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| C327 | S-Nitrosylation | Uniprot | |
| T328 | Phosphorylation | Uniprot |
Research Backgrounds
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Poly-ADP-ribosylated by PARP1.
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.
Belongs to the WD repeat BUB3 family.
Research Fields
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.