MSHR Antibody | Affinity Biosciences

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Western blot analysis of MSHR Antibody expression in A375 cells lysates.

Product:	MSHR Antibody
Catalog:	AF5251
Description:	Rabbit polyclonal antibody to MSHR
Application:	WB IF/ICC
Reactivity:	Human
Prediction:	Chicken
Mol.Wt.:	35 kDa; 35kD(Calculated).
Uniprot:	Q01726
RRID:	AB_2837737

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Chicken(82%)

Clonality:

Polyclonal

Specificity:

MSHR Antibody detects endogenous levels of total MSHR.

RRID:

AB_2837737
Cite Format: Affinity Biosciences Cat# AF5251, RRID:AB_2837737.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CMM5; MC1-R; MC1R; Melanocortin 1 receptor; Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor); Melanocortin receptor 1; Melanocyte-stimulating hormone receptor; Melanotropin receptor; MSH-R; MSHR; MSHR_HUMAN; SHEP2;

Immunogens

Immunogen:

Uniprot:

Q01726(MSHR_HUMAN) >>Visit HPA database.

Gene(ID):

MC1R(4157)

Expression:

Q01726 MSHR_HUMAN:

Melanocytes and corticoadrenal tissue.

Description:

Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

Sequence:

Q01726 MSHR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAVQGSQRRLLGSLNSTPTAIPQLGLAANQTGARCLEVSISDGLFLSLGLVSLVENALVVATIAKNRNLHSPMYCFICCLALSDLLVSGSNVLETAVILLLEAGALVARAAVLQQLDNVIDVITCSSMLSSLCFLGAIAVDRYISIFYALRYHSIVTLPRARRAVAAIWVASVVFSTLFIAYYDHVAVLLCLVVFFLAMLVLMAVLYVHMLARACQHAQGIARLHKRQRPVHQGFGLKGAVTLTILLGIFFLCWGPFFLHLTLIVLCPEHPTCGCIFKNFNLFLALIICNAIIDPLIYAFHSQELRRTLKEVLTCSW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Chicken

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01726 As Substrate

Q01726

Site	PTM Type	Source
T62	Phosphorylation	Uniprot
S154	Phosphorylation	Uniprot
T157	Phosphorylation	Uniprot
T308	Phosphorylation	Uniprot
S316	Phosphorylation	Uniprot

Research Backgrounds

Q01726_MSHR_HUMAN

Function:

Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Melanocytes and corticoadrenal tissue.

Subunit Structure:

Interacts with MGRN1, but does not undergo MGRN1-mediated ubiquitination; this interaction competes with GNAS-binding and thus inhibits agonist-induced cAMP production.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Melanogenesis.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MSHR Antibody - #AF5251