VEGFB Antibody - #AF5250

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Product: VEGFB Antibody
Catalog: AF5250
Description: Rabbit polyclonal antibody to VEGFB
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: P49765
RRID: AB_2837736

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(88%)
Clonality:
Polyclonal
Specificity:
VEGFB Antibody detects endogenous levels of total VEGFB.
RRID:
AB_2837736
Cite Format: Affinity Biosciences Cat# AF5250, RRID:AB_2837736.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Vascular endothelial growth factor B; Vascular endothelial growth factor-related factor; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF;

Immunogens

Immunogen:
Uniprot:

P49765(VEGFB_HUMAN) >>Visit HPA database.

Gene(ID):
VEGFB(7423)
Expression:
P49765 VEGFB_HUMAN:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Description:
Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.
Sequence:
MSPLLRRLLLAALLQLAPAQAPVSQPDAPGHQRKVVSWIDVYTRATCQPREVVVPLTVELMGTVAKQLVPSCVTVQRCGGCCPDDGLECVPTGQHQVRMQILMIRYPSSQLGEMSLEEHSQCECRPKKKDSAVKPDRAATPHHRPQPRSVPGWDSAPGAPSPADITHPTPAPGPSAHAAPSTTSALTPGPAAAAADAAASSVAKGGA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Pig
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis.

PTMs:

VEGF-B186 is O-glycosylated.

Subcellular Location:

Secreted.
Note: Secreted but remains associated to cells or to the extracellular matrix unless released by heparin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas.

Subunit Structure:

Homodimer; disulfide-linked. Can also form heterodimer with VEGF.

Family&Domains:

Belongs to the PDGF/VEGF growth factor family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

References

1). Reducing VEGFB expression regulates the balance of glucose and lipid metabolism in mice via VEGFR1. Molecular Medicine Reports, 2022 (PubMed: 35894135) [IF=3.4]

Application: WB    Species: Mouse    Sample:

Figure 1. - Systemic VEGFB-knockdown mouse model construction and genetic identification. (A) VEGFB gene structure, gRNA target site and deletion region. Boxes and lines respectively represent exons and introns. F1 and R1 arrows indicate the position of primers for PCR genotyping. 1–7 shows the VEGFB gene sequence, where the red line represents the VEGFB knockout gene sequence. (B-E) Western blot analysis of VEGFB protein in liver tissues and pancreas. (F and G) mRNA analysis in the liver tissues and pancreas. **P<0.01 vs. VEGFB+/+ group and ***P<0.001 vs. VEGFB+/+ group. VEGFB, vascular endothelial growth factor B.

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