GATA4 Antibody - #AF5245

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Product: GATA4 Antibody
Catalog: AF5245
Description: Rabbit polyclonal antibody to GATA4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 46 kDa; 45kD(Calculated).
Uniprot: P43694
RRID: AB_2837731

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
GATA4 Antibody detects endogenous levels of total GATA4.
RRID:
AB_2837731
Cite Format: Affinity Biosciences Cat# AF5245, RRID:AB_2837731.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ASD2; GATA 4; GATA binding protein 4; GATA-binding factor 4; gata4; GATA4_HUMAN; MGC126629; Transcription factor GATA 4; Transcription factor GATA-4; Transcription factor GATA4; VSD1;

Immunogens

Immunogen:
Uniprot:

P43694(GATA4_HUMAN) >>Visit HPA database.

Gene(ID):
GATA4(2626)
Description:
Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development. Acts as a transcriptional activator of ANF in cooperation with NKX2-5. Promotes cardiac myocyte enlargement. Required during testicular development.
Sequence:
MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43694 As Substrate

Site PTM Type Enzyme
S105 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3) , P11802 (CDK4)
S109 Phosphorylation
Y236 Phosphorylation
Y244 Phosphorylation
S262 Phosphorylation P17612 (PRKACA)
S264 Phosphorylation
S395 Phosphorylation
S401 Phosphorylation
S406 Phosphorylation
S412 Phosphorylation

Research Backgrounds

Function:

Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function. In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it downregulates endocardial and endothelial gene expression. Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement. Required during testicular development. May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase.

PTMs:

Methylation at Lys-300 attenuates transcriptional activity.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its ability to activate transcription of ANF. Interacts (via the second Zn finger) with NFATC4 (By similarity). Interacts with LMCD1 (By similarity). Forms a complex made of CDK9, CCNT1/cyclin-T1, EP300 and GATA4 that stimulates hypertrophy in cardiomyocytes. Interacts with NR5A1, ZFPM2 and TBX5. Interacts with TBX18.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

References

1). Effect of miR-20b on Apoptosis, Differentiation, the BMP Signaling Pathway and Mitochondrial Function in the P19 Cell Model of Cardiac Differentiation In Vitro. PLoS One, 2015 (PubMed: 25898012) [IF=3.7]

Application: WB    Species:    Sample: miR-20b cells

Fig 7. |Effects of miR-20b on the BMP signaling pathway, as assessed by by western blotting. a): in the miR-20b overexpression group; b): in miR-20b silenced group. (n = 3, *: P<0.05, **: P<0.01, ***: P<0.001)
2). BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells. Theriogenology, 2023 (PubMed: 36525856) [IF=2.8]
3). Identification of key microRNAs and the underlying molecular mechanism in spinal cord ischemia-reperfusion injury in rats. PeerJ, 2021 (PubMed: 34123589) [IF=2.7]

Application: WB    Species: Rat    Sample: spinal cord tissues

Figure 8 Effects of AMO-3568 on the protein expression levels of RPBJ (A, B), GATA6 (A, C) and GATA4 (A, D) were measured by Western blot assay. n = 4 for per group. Data were analyzed by one-way ANOVA followed by the Tukey’s test. *p < 0.05, versus the sham group, #p < 0.05 versus the SCII group.
4). Effective isolation of Sertoli cells from New Zealand rabbit testis. Journal of Advanced Veterinary and Animal Research, 2021 (PubMed: 34395591) [IF=1.4]
5). Pathological and Immunohistochemical Analysis of Splenic Metastasis of Adrenal Cortical Carcinoma in a Cat. 畜牧兽医学报, 2021

Application: IHC    Species: Cat    Sample:

A. Synaptophysin在肿瘤组织胞质中呈阳性表达，且阳性区域广泛, bar=200 μm；B. Synaptophysin在肿瘤组织胞质中呈阳性表达(黑色箭头)，bar=100 μm；C.S-100在肿瘤细胞中表达为少量细胞阳性反应(黑色箭头)，bar=100 μm；D. GATA4在肿瘤组织细胞核中表达为阳性反应(黑色箭头)，bar=100 μm；E. Pancytokeratin在肿瘤组织中表达为阴性反应，bar=200 μm；F. Vimentin在肿瘤组织中表达为阴性反应，bar=200 μm A. Synaptophysin was positively expressed in the cytoplasm of tumor tissues, and the positive region was extensive bar=200 μm; B. Synaptophysin was positively expressed in the cytoplasm of tumor tissue (black arrow), bar=100 μm; C. S-100 was expressed as a small amount of cell positive reaction in tumor cells (black arrow), bar=100 μm; D. GATA4 was positively expressed in the nucleus of tumor tissues (black arrow), bar=100 μm; E. Pancytokeratin was negatively expressed in tumor tissues, bar=200 μm; F. Vimentin was negative in tumor tissue, bar=200 μm 图 3 免疫组化结果 Fig. 3 Immunohistochemical results
6). Formate assay in body fluids: application in methanol poisoning. Biochemical medicine, 1975 (PubMed: 1)

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