FSHR Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/6

Western blot analysis of FSHR expression in HUVEC cell extracts.

Western blot analysis of extracts from Mouse liver, using FSHR Antibody.

AF5242 at 1/100 staining Mouse testis tissue by IHC-P.

AF5242 staining Hela by IF/ICC.

Figure 3.

Figure 1 Identification of yak follicular granulosa cells via FSHR immunofluorescence.

Product:	FSHR Antibody
Catalog:	AF5242
Description:	Rabbit polyclonal antibody to FSHR
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	78 kDa; 78kD(Calculated).
Uniprot:	P23945
RRID:	AB_2837728

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

FSHR Antibody detects endogenous levels of total FSHR.

RRID:

AB_2837728
Cite Format: Affinity Biosciences Cat# AF5242, RRID:AB_2837728.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Follicle stimulating hormone receptor; Follicle stimulating hormone receptor isoform 1; Follicle-stimulating hormone receptor; Follitropin receptor; FSH receptor; FSH-R; Fshr; FSHR_HUMAN; FSHRO; LGR1; MGC141667; MGC141668; ODG1; ovarian dysgenesis 1;

Immunogens

Immunogen:

Uniprot:

P23945(FSHR_HUMAN) >>Visit HPA database.

Gene(ID):

FSHR(2492)

Expression:

P23945 FSHR_HUMAN:

Sertoli cells and ovarian granulosa cells.

Description:

Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

Sequence:

P23945 FSHR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALLLVSLLAFLSLGSGCHHRICHCSNRVFLCQESKVTEIPSDLPRNAIELRFVLTKLRVIQKGAFSGFGDLEKIEISQNDVLEVIEADVFSNLPKLHEIRIEKANNLLYINPEAFQNLPNLQYLLISNTGIKHLPDVHKIHSLQKVLLDIQDNINIHTIERNSFVGLSFESVILWLNKNGIQEIHNCAFNGTQLDELNLSDNNNLEELPNDVFHGASGPVILDISRTRIHSLPSYGLENLKKLRARSTYNLKKLPTLEKLVALMEASLTYPSHCCAFANWRRQISELHPICNKSILRQEVDYMTQARGQRSSLAEDNESSYSRGFDMTYTEFDYDLCNEVVDVTCSPKPDAFNPCEDIMGYNILRVLIWFISILAITGNIIVLVILTTSQYKLTVPRFLMCNLAFADLCIGIYLLLIASVDIHTKSQYHNYAIDWQTGAGCDAAGFFTVFASELSVYTLTAITLERWHTITHAMQLDCKVQLRHAASVMVMGWIFAFAAALFPIFGISSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYIHIYLTVRNPNIVSSSSDTRIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKAKILLVLFHPINSCANPFLYAIFTKNFRRDFFILLSKCGCYEMQAQIYRTETSSTVHNTHPRNGHCSSAPRVTNGSTYILVPLSHLAQN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Chicken

Xenopus

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23945 As Substrate

P23945

Site	PTM Type	Source
K140	Acetylation	Uniprot
N191	N-Glycosylation	Uniprot
S218	Phosphorylation	Uniprot
S226	Phosphorylation	Uniprot
T228	Phosphorylation	Uniprot
Y250	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
S660	Phosphorylation	Uniprot

Research Backgrounds

P23945_FSHR_HUMAN

Function:

G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

PTMs:

Sulfated.

N-glycosylated; indirectly required for FSH-binding, possibly via a conformational change that allows high affinity binding of hormone.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Sertoli cells and ovarian granulosa cells.

Subunit Structure:

Homotrimer. Functions as a homotrimer binding the FSH hormone heterodimer composed of CGA and FSHB. Interacts with ARRB2 (By similarity). Interacts with APPL2; interaction is independent of follicle stimulating hormone stimulation.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

References

1). Study on the effects and mechanisms of Wenzhong Bushen Formula in improving ovarian reserve decline in mice based on network pharmacology. Journal of ethnopharmacology, 2024 (PubMed: 38218503) [IF=5.4]

2). Resveratrol Promotes Proliferation, Antioxidant Properties, and Progesterone Production in Yak (Bos grunniens) Granulosa Cells. Animals : an open access journal from MDPI, 2024 (PubMed: 38254409) [IF=3.0]

Application: IF/ICC Species: Yak Sample: GCs

Figure 1 Identification of yak follicular granulosa cells via FSHR immunofluorescence. Second-generation cells were used in the experiment. Red fluorescence indicates the specific expression of FSHR, and blue fluorescence indicates the nucleus. Bar = 20 μm.

3). Regulation of proliferation, apoptosis, hormone secretion and gene expression by acetyl-L-carnitine in yak (Bos grunniens) granulosa cells. Theriogenology, 2023 (PubMed: 36972666) [IF=2.8]

4). Difference in Expressional Profile of Compact and Expanded-Type Equine Cumulus-Oocyte-Complexes. , 2022

5). Characterization and establishment of an immortalized rabbit ovary granulosa cell line for reproductive biology experiments. Research Square, 2022

Application: WB Species: unknown Sample:

Figure 3

6). Study on the Effects and Mechanisms of Wenzhong Bushen Formula in Improving Ovarian Reserve Decline in Mice Based on Network Pharmacology.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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FSHR Antibody - #AF5242