hnRNP H Antibody - #AF5241

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Product: hnRNP H Antibody
Catalog: AF5241
Description: Rabbit polyclonal antibody to hnRNP H
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 49 kDa; 49kD(Calculated).
Uniprot: P31943
RRID: AB_2837727

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
hnRNP H Antibody detects endogenous levels of total hnRNP H.
RRID:
AB_2837727
Cite Format: Affinity Biosciences Cat# AF5241, RRID:AB_2837727.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp686A15170; Heterogeneous nuclear ribonucleoprotein H; Heterogeneous nuclear ribonucleoprotein H1 (H); Heterogeneous nuclear ribonucleoprotein H1; HNRH1_HUMAN; hnRNP H; hnRNPH; Hnrnph1; HNRPH 1; HNRPH; HNRPH1 protein; N-terminally processed;

Immunogens

Immunogen:
Uniprot:

P31943(HNRH1_HUMAN) >>Visit HPA database.

Gene(ID):
HNRNPH1(3187)
Expression:
P31943 HNRH1_HUMAN:

Expressed ubiquitously.

Description:
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
Sequence:
MMLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
73
Zebrafish
64
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31943 As Substrate

Site PTM Type Enzyme
M1 Acetylation
M2 Acetylation
T5 Phosphorylation
K14 Sumoylation
K14 Ubiquitination
S21 Phosphorylation
S23 Phosphorylation
R29 Methylation
C34 S-Nitrosylation
K35 Sumoylation
K35 Ubiquitination
R44 Methylation
Y47 Phosphorylation
S54 Phosphorylation
S63 Phosphorylation
K68 Sumoylation
K72 Sumoylation
R81 Methylation
K87 Sumoylation
K87 Ubiquitination
S88 Phosphorylation
K98 Acetylation
K98 Sumoylation
K98 Ubiquitination
T100 Phosphorylation
S104 Phosphorylation P28482 (MAPK1) , P24941 (CDK2)
T107 Phosphorylation
R114 Methylation
C122 S-Nitrosylation
S123 Phosphorylation
S151 Phosphorylation
T152 O-Glycosylation
S161 Phosphorylation
K167 Acetylation
K167 Ubiquitination
K170 Ubiquitination
K171 Ubiquitination
Y180 Phosphorylation
K185 Acetylation
K185 Methylation
K185 Sumoylation
K185 Ubiquitination
S186 Phosphorylation
S187 Phosphorylation
T193 Phosphorylation
Y195 Phosphorylation
R199 Methylation
K200 Ubiquitination
R206 Methylation
Y210 Phosphorylation
R212 Methylation
R217 Methylation
Y219 Phosphorylation
S221 Phosphorylation
R224 Methylation
R230 Methylation
R232 Methylation
R233 Methylation
Y236 Phosphorylation
Y240 Phosphorylation
Y243 Phosphorylation
Y246 Phosphorylation
Y249 Phosphorylation
Y253 Phosphorylation
S257 Phosphorylation
R259 Methylation
R262 Methylation
Y266 Phosphorylation
C267 S-Nitrosylation
S269 Phosphorylation
S272 Phosphorylation
Y276 Phosphorylation
T286 Phosphorylation
R294 Methylation
T301 Phosphorylation
Y306 Phosphorylation
S310 Phosphorylation
T328 Phosphorylation
S346 Phosphorylation
K349 Ubiquitination
S368 Phosphorylation
Y372 Phosphorylation
Y376 Phosphorylation

Research Backgrounds

Function:

This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).

Subcellular Location:

Nucleus>Nucleoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed ubiquitously.

Subunit Structure:

Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRNPH1. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with CUGBP1; the interaction is RNA-dependent. Interacts with MBNL1; the interaction in RNA-independent.

Family&Domains:

Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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