INSL3 Antibody | Affinity Biosciences

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Western blot analysis of INSL3 expression in MCF7 cells,The lane on the left was treated with the antigen-specific peptide.

Product:	INSL3 Antibody
Catalog:	AF5227
Description:	Rabbit polyclonal antibody to INSL3
Application:	WB
Reactivity:	Human
Prediction:	Pig, Dog
Mol.Wt.:	14 kDa; 15kD(Calculated).
Uniprot:	P51460
RRID:	AB_2837713

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

INSL3 Antibody detects endogenous levels of total INSL3.

RRID:

AB_2837713
Cite Format: Affinity Biosciences Cat# AF5227, RRID:AB_2837713.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

INSL 3; INSL3; INSL3_HUMAN; Insulin like 3; insulin like peptide, Leydig cell-specific; insulin-like 3 (Leydig cell); Insulin-like 3 A chain; Ley IL; Ley-I-L; leydig insulin -like hormone; Leydig insulin like peptide; Leydig insulin-like peptide; prepro-INSL3; Relaxin like factor; Relaxin-like factor; relaxin-like factor b; RLF; RLNL;

Immunogens

Immunogen:

Uniprot:

P51460(INSL3_HUMAN) >>Visit HPA database.

Gene(ID):

INSL3(3640)

Expression:

P51460 INSL3_HUMAN:

Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast.

Description:

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor. Heterodimer of a B chain and an A chain linked by two disulfide bonds

Sequence:

P51460 INSL3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPRLPAWALVLLGPALVFALGPAPTPEMREKLCGHHFVRALVRVCGGPRWSTEARRPATGGDRELLQWLERRHLLHGLVADSNLTLGPGLQPLPQTSHHHRHHRAAATNPARYCCLSGCTQQDLLTLCPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Dog

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P51460_INSL3_HUMAN

Function:

Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor.

Subcellular Location:

Secreted.

Tissue Specificity:

Expressed in prenatal and postnatal Leydig cells. Found as well in the corpus luteum, trophoblast, fetal membranes and breast.

Subunit Structure:

Heterodimer of a B chain and an A chain linked by two disulfide bonds.

Family&Domains:

Belongs to the insulin family.

Research Fields

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

INSL3 Antibody - #AF5227