AQP3 Antibody - #AF5222

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Product: AQP3 Antibody
Catalog: AF5222
Description: Rabbit polyclonal antibody to AQP3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 31 kDa(glyco AQP3~ 45kd); 32kD(Calculated).
Uniprot: Q92482
RRID: AB_2837708

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
AQP3 Antibody detects endogenous levels of total AQP3.
RRID:
AB_2837708
Cite Format: Affinity Biosciences Cat# AF5222, RRID:AB_2837708.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AQP 3; AQP-3; Aqp3; AQP3_HUMAN; Aquaglyceroporin-3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin-3; Aquaporin3; GIL; Gill blood group;

Immunogens

Immunogen:
Uniprot:

Q92482(AQP3_HUMAN) >>Visit HPA database.

Gene(ID):
AQP3(360)
Expression:
Q92482 AQP3_HUMAN:

Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.

Description:
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Sequence:
MGRQKELVSRCGEMLHIRYRLLRQALAECLGTLILVMFGCGSVAQVVLSRGTHGGFLTINLAFGFAVTLGILIAGQVSGAHLNPAVTFAMCFLAREPWIKLPIYTLAQTLGAFLGAGIVFGLYYDAIWHFADNQLFVSGPNGTAGIFATYPSGHLDMINGFFDQFIGTASLIVCVLAIVDPYNNPVPRGLEAFTVGLVVLVIGTSMGFNSGYAVNPARDFGPRLFTALAGWGSAVFTTGQHWWWVPIVSPLLGSIAGVFVYQLMIGCHLEQPPPSNEEENVKLAHVKHKEQI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92482 As Substrate

Site PTM Type Enzyme
K5 Ubiquitination
K100 Sumoylation
K287 Ubiquitination

Research Backgrounds

Function:

Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism (By similarity).

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.

Family&Domains:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Research Fields

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

References

1). Daidzein Protects Caco-2 Cells against Lipopolysaccharide-Induced Intestinal Epithelial Barrier Injury by Suppressing PI3K/AKT and P38 Pathways. MOLECULES, 2022 (PubMed: 36558058) [IF=4.6]

Application: WB    Species: Human    Sample:

Figure 3. Daidzein increased lipopolysaccharide (LPS)-induced downregulated expression of tight junction and AQP3 proteins and mRNA. (A) The protein expression levels of ZO-1 and occludin and protein quantitative analysis. (B) The protein expression levels of claudin-1 and AQP3 and protein quantitative analysis. (C–F) The mRNA expression levels of ZO-1, occludin, claudin-1, and AQP3 (mean ± SD, n = 3), * p < 0.05, ** p < 0.01, and *** p < 0.001 versus LPS-treated alone. # p < 0.05, ## p < 0.01, and ### p < 0.001 versus control.
2). Ameliorative effects of the mixed aqueous extract of Aurantii Fructus Immaturus and Magnoliae Officinalis Cortex on loperamide-induced STC mice. Heliyon, 2024 (PubMed: 39040398) [IF=4.0]
3). Altered Expressions of AQP3 and ADP Are Closely Related with the Risk of Preeclampsia Occurrence. GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 2020 (PubMed: 32957104) [IF=2.1]

Application: IHC    Species: human    Sample: placenta

Fig. 1. |Expression of AQP3 protein in the placenta (I, II) and fetal membranes (III, IV) shown in IHC and (I, III)Western blotting (I, IV) images.

Application: WB    Species: human    Sample: placenta

Fig. 1. |Expression of AQP3 protein in the placenta (I, II) and fetal membranes (III, IV) shown in IHC and (I, III)Western blotting (I, IV) images.
4). Hippophae rhamnoides L. leaf extract diminishes oxidative stress, inflammation and ameliorates bioenergetic activation in full-thickness burn wound healing. Phytomedicine Plus, 2022

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