Product: CD147 Antibody
Catalog: AF5221
Description: Rabbit polyclonal antibody to CD147
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep, Rabbit, Dog
Mol.Wt.: 42 kDa, 70 kDa; 42kD(Calculated).
Uniprot: P35613
RRID: AB_2837707

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Sheep(88%), Rabbit(89%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CD147 Antibody detects endogenous levels of total CD147.
RRID:
AB_2837707
Cite Format: Affinity Biosciences Cat# AF5221, RRID:AB_2837707.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5A11 antigen; 5F7; BASI_HUMAN; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; Bsg; CD 147; CD147; CD147 antigen; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M 6; M6; M6 leukocyte activation antigen; Neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; Tumor cell-derived collagenase stimulatory factor;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P35613 BASI_HUMAN:

Expressed in erythrocytes (at protein level) (PubMed:28409866, PubMed:26195724). Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Description:
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).
Sequence:
MAAALFVLLGFALLGTHGASGAAGFVQAPLSQQRWVGGSVELHCEAVGSPVPEIQWWFEGQGPNDTCSQLWDGARLDRVHIHATYHQHAASTISIDTLVEEDTGTYECRASNDPDRNHLTRAPRVKWVRAQAVVLVLEPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Rabbit
89
Sheep
88
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35613 As Substrate

Site PTM Type Enzyme
T145 O-Glycosylation
T156 Phosphorylation
N160 N-Glycosylation
K173 Ubiquitination
K179 Ubiquitination
K187 Ubiquitination
K191 Ubiquitination
K224 Ubiquitination
K227 Ubiquitination
S228 Phosphorylation
T237 Phosphorylation
C242 S-Nitrosylation
K243 Ubiquitination
K257 Sumoylation
K257 Ubiquitination
K264 Ubiquitination
N268 N-Glycosylation
S279 Phosphorylation
Y299 Phosphorylation
K307 Ubiquitination
S309 Phosphorylation
T315 Phosphorylation
K350 Ubiquitination
S362 Phosphorylation
K366 Ubiquitination
S367 Phosphorylation
S368 Phosphorylation
K375 Ubiquitination

Research Backgrounds

Function:

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8, SLC16A11 and SLC16A12 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

May induce the production of pro-inflammatory cytokines such as IL6 in monocytes.

(Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3. Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte. This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In spermatozoa, localized on the principal piece of caput and in the middle piece during transit in the corpus and cauda epididymides (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in erythrocytes (at protein level). Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Subunit Structure:

Forms homooligomers in a cis-dependent manner on the plasma membrane. Forms heterooligomers of isoform 2 and isoform 3. Forms a complex with MMP1 at the tumor cell surface. Interacts with ATP1B2, MAG and L1CAM (By similarity). Interacts with AJAP1. Interacts with SLC16A1, SLC16A7 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. Interacts with PPIL2; regulates BSG transport to the cell membrane. Interacts with SLC16A11. Interacts with SLC16A12.

(Microbial infection) Interacts with P.falciparum (isolates 3D7 or 7G8) RH5/PfRH5; the interaction is required for the invasion of the host erythrocytes by the parasite at the merozoite stage.

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