NCF1/p47-phox Antibody - #AF5220

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Product: NCF1/p47-phox Antibody
Catalog: AF5220
Description: Rabbit polyclonal antibody to NCF1/p47-phox
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 47 kDa; 45kD(Calculated).
Uniprot: P14598
RRID: AB_2837706

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
NCF1/p47-phox Antibody detects endogenous levels of total NCF1/p47-phox.
RRID:
AB_2837706
Cite Format: Affinity Biosciences Cat# AF5220, RRID:AB_2837706.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF 47K; NCF-1; NCF-47K; Ncf1; NCF1_HUMAN; Neutrophil cytosol factor 1; Neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox organizing protein 2; Nox-organizing protein 2; NOXO2; p47 phox; p47-phox; SH3 and PX domain containing protein 1A; SH3 and PX domain-containing protein 1A; SH3PXD1A;

Immunogens

Immunogen:
Uniprot:

P14598(NCF1_HUMAN) >>Visit HPA database.

Gene(ID):
NCF1(653361)
Expression:
P14598 NCF1_HUMAN:

Detected in peripheral blood monocytes and neutrophils (at protein level).

Description:
Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG.
Sequence:
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEFHKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCSTLMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTATDITGPIILQTYRAIANYEKTSGSEMALSTGDVVEVVEKSESGWWFCQMKAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLLEGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGAPPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGSPLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
89
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14598 As Substrate

Site PTM Type Enzyme
Y41 Phosphorylation
T45 Phosphorylation
Y48 Phosphorylation
T133 Phosphorylation Q9NWZ3 (IRAK4)
T138 Phosphorylation Q9NWZ3 (IRAK4)
Y139 Phosphorylation
T153 Phosphorylation Q9NWZ3 (IRAK4)
S208 Phosphorylation P68400 (CSNK2A1)
Y279 Phosphorylation
S283 Phosphorylation P68400 (CSNK2A1)
S288 Phosphorylation Q9NWZ3 (IRAK4)
S303 Phosphorylation P17252 (PRKCA) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB)
S304 Phosphorylation Q05513 (PRKCZ) , P05771-2 (PRKCB) , P17252 (PRKCA) , P31749 (AKT1) , Q05655 (PRKCD)
S310 Phosphorylation
S315 Phosphorylation P05771 (PRKCB) , P17252 (PRKCA) , Q05513 (PRKCZ) , Q05655 (PRKCD)
S320 Phosphorylation Q9NWZ3 (IRAK4) , P17612 (PRKACA) , P05771-2 (PRKCB) , Q05513 (PRKCZ) , Q05655 (PRKCD) , P17252 (PRKCA)
S328 Phosphorylation Q05655 (PRKCD) , P31749 (AKT1) , P17252 (PRKCA) , P17612 (PRKACA) , Q05513 (PRKCZ) , P05771 (PRKCB)
S345 Phosphorylation Q16539 (MAPK14) , Q9NWZ3 (IRAK4) , P28482 (MAPK1) , P27361 (MAPK3)
S348 Phosphorylation P68400 (CSNK2A1) , P28482 (MAPK1) , P27361 (MAPK3) , Q9NWZ3 (IRAK4) , Q16539 (MAPK14)
T356 Phosphorylation Q9NWZ3 (IRAK4)
S359 Phosphorylation Q9NWZ3 (IRAK4) , Q05513 (PRKCZ) , P05771-2 (PRKCB) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA)
S370 Phosphorylation Q05513 (PRKCZ) , Q9NWZ3 (IRAK4) , P05771 (PRKCB) , Q05655 (PRKCD) , P17612 (PRKACA) , P17252 (PRKCA)
S379 Phosphorylation Q05513 (PRKCZ) , Q05655 (PRKCD) , P05771 (PRKCB) , P17252 (PRKCA)
S381 Phosphorylation
T382 Phosphorylation

Research Backgrounds

Function:

NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

PTMs:

Phosphorylated by PRKCD; phosphorylation induces activation of NCF1 and NADPH oxidase activity.

Subcellular Location:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in peripheral blood monocytes and neutrophils (at protein level).

Subunit Structure:

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts (via C-terminus) with NCF2 (via the C-terminal SH3 domain). Interacts with NCF4. Interacts with CYBB. Interacts (via the second SH3 domain) with CYBA. Interacts with NOXA1. Interacts with ADAM15. Interacts with TRAF4. Interacts with FASLG. Interacts with PARK7 (via C-terminus); the interaction is enhanced by LPS and modulates NCF1 phosphorylation and membrane translocation (By similarity).

Family&Domains:

The PX domain mediates interaction with phosphatidylinositol 3,4-bisphosphate and other anionic phospholipids. In the autoinhibited, unphosphorylated state an intramolecular interaction with the C-terminal SH3 domain precludes phospholipid binding and interaction with CYBA. Phosphorylation disrupts the autoinhibited state.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway. Cell Death & Disease, 2020 (PubMed: 32829380) [IF=9.0]

Application: WB    Species: mouse    Sample: renal cortex

Fig. 5 Effects of ELA on DOCA/salt-induced NADPH oxidase formation and activation. a mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal cortex. b mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal medulla. c Representative immunoblots of p22phox and p47phox and summarized intensities of blots. d Intracellular NADP+/NADPH in renal cortex and medulla. N = 6 per group. *P < 0.05 versus Ctrl group; #P < 0.05 versus DOCA/salt group.
2). Aqueous extract of Phellinus igniarius ameliorates hyperuricemia and renal injury in adenine/potassium oxonate-treated mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38879892) [IF=7.5]
3). β-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinase–mediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production. Journal of Dairy Science, 2022 (PubMed: 35123783) [IF=3.5]
4). Free fatty acids promote degranulation of azurophil granules in neutrophils by inducing production of NADPH oxidase–derived reactive oxygen species in cows with subclinical ketosis. Journal of Dairy Science, 2022 (PubMed: 34998570) [IF=3.5]
5). Comprehensive analysis and identification of prognostic biomarkers and immunotherapeutic targets in the NADPH oxidase family (and its regulatory subunits) in pancreatic ductal adenocarcinoma. Clinical and Translational Oncology, 2023 [IF=3.4]

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