GPR120 Antibody - #AF5219

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Product: GPR120 Antibody
Catalog: AF5219
Description: Rabbit polyclonal antibody to GPR120
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 42 kDa; 42kD(Calculated).
Uniprot: Q5NUL3
RRID: AB_2837705

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Horse(90%), Sheep(90%), Rabbit(90%), Dog(91%)
Clonality:
Polyclonal
Specificity:
GPR120 Antibody detects endogenous levels of total GPR120.
RRID:
AB_2837705
Cite Format: Affinity Biosciences Cat# AF5219, RRID:AB_2837705.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

G protein coupled receptor 120; G protein coupled receptor 129; G protein coupled receptor GT01; G protein coupled receptor PGR 4; G protein coupled receptor PGR4; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; GPR 120; GPR 129; GPR120; GPR129; GT01; HGNC:19345; MGC119984; O3FA1_HUMAN; O3FAR1; Omega-3 fatty acid receptor 1; PGR 4; PGR4;

Immunogens

Immunogen:
Uniprot:

Q5NUL3(FFAR4_HUMAN) >>Visit HPA database.

Gene(ID):
FFAR4(338557)
Expression:
Q5NUL3 FFAR4_HUMAN:

Abundant expression in the intestinal tract. Highly expressed in adipose tissue, small intestine and pancreas.

Description:
GPR120, a member of the rhodopsin family of G protein-coupled receptors (GPCRs), is a 377 amino acid protein which is expressed in the intestine. GPR120 is a receptor for unsaturated long-chain FFAs (free fatty acids). FFAs act as signaling molecules and are an important energy source
Sequence:
MSPECARAAGDAPLRSLEQANRTRFPFFSDVKGDHRLVLAAVETTVLVLIFAVSLLGNVCALVLVARRRRRGATACLVLNLFCADLLFISAIPLVLAVRWTEAWLLGPVACHLLFYVMTLSGSVTILTLAAVSLERMVCIVHLQRGVRGPGRRARAVLLALIWGYSAVAALPLCVFFRVVPQRLPGADQEISICTLIWPTIPGEISWDVSFVTLNFLVPGLVIVISYSKILQTSEHLLDARAVVTHSEITKASRKRLTVSLAYSESHQIRVSQQDFRLFRTLFLLMVSFFIMWSPIIITILLILIQNFKQDLVIWPSLFFWVVAFTFANSALNPILYNMTLCRNEWKKIFCCFWFPEKGAILTDTSVKRNDLSIISG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Horse
90
Bovine
90
Sheep
90
Rabbit
90
Chicken
70
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5NUL3 As Substrate

Site PTM Type Enzyme
S253 Phosphorylation
T258 Phosphorylation
Y263 Phosphorylation
S266 Phosphorylation
T363 Phosphorylation
S366 Phosphorylation

Research Backgrounds

Function:

Receptor for medium and long-chain free fatty acids (FFAs). Signals via a G(q)/G(11)-coupled pathway. Acts as a receptor for omega-3 fatty acids and mediates robust anti-inflammatory effects, particularly in macrophages and fat cells. The anti-inflammatory effects involve inhibition of TAK1 through a beta-arrestin 2 (ARRB2)/TAB1-dependent effect, but independent of the G(q)/G(11)-coupled pathway. Mediates potent insulin sensitizing and antidiabetic effects by repressing macrophage-induced tissue inflammation. May mediate the taste of fatty acids. Mediates FFA-induced inhibition of apoptosis in enteroendocrine cells. May play a role in the regulation of adipocyte development and differentiation.

PTMs:

Phosphorylated. FFA stimulation facilitates phosphorylation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Colocalized with ARRB2 following DHA treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant expression in the intestinal tract. Highly expressed in adipose tissue, small intestine and pancreas.

Subunit Structure:

Interacts with ARRB2 following docosahexaenoic acid (DHA) stimulation.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

References

1). Different n-6/n-3 PUFA diets with fish oil attenuated osteoarthritis in ovariectomized mice via targeting the NLRP3 inflammasome. Food Bioscience, 2022 [IF=5.2]
2). Stearic acid induces CCK and GLP-1 upregulation via GPR120/PLC-β, leading to reduced appetite in Hu sheep fed with rice straw. Frontiers in Veterinary Science, 2022 [IF=3.2]

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