Product: Claudin 6 Antibody
Catalog: AF5213
Description: Rabbit polyclonal antibody to Claudin 6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P56747
RRID: AB_2837699

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
Claudin 6 Antibody detects endogenous levels of total Claudin 6.
RRID:
AB_2837699
Cite Format: Affinity Biosciences Cat# AF5213, RRID:AB_2837699.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Claudin-6; Claudin6; CLD6_HUMAN; CLDN 6; CLDN6; OTTHUMP00000159248; Skullin 2; Skullin; UNQ757/PRO1488;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P56747 CLD6_HUMAN:

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

Description:
Plays a major role in tight junction-specific obliteration of the intercellular space.
Sequence:
MASAGMQILGVVLTLLGWVNGLVSCALPMWKVTAFIGNSIVVAQVVWEGLWMSCVVQSTGQMQCKVYDSLLALPQDLQAARALCVIALLVALFGLLVYLAGAKCTTCVEEKDSKARLVLTSGIVFVISGVLTLIPVCWTAHAIIRDFYNPLVAEAQKRELGASLYLGWAASGLLLLGGGLLCCTCPSGGSQGPSHYMARYSTSAPAISRGPSEYPTKNYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Pig
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56747 As Substrate

Site PTM Type Enzyme
Y200 Phosphorylation
S201 Phosphorylation
T202 Phosphorylation
S203 Phosphorylation
S208 Phosphorylation
S212 Phosphorylation
Y214 Phosphorylation
T216 Phosphorylation
K217 Ubiquitination
Y219 Phosphorylation

Research Backgrounds

Function:

Plays a major role in tight junction-specific obliteration of the intercellular space.

(Microbial infection) Acts as a receptor for hepatitis C virus (HCV) entry into hepatic cells.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the liver, in peripheral blood mononuclear cells and hepatocarcinoma cell lines.

Subunit Structure:

Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with CLDN1, CD81 and OCLN.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Downregulation of CLDN6 inhibits cell migration and invasion and promotes apoptosis by regulation of the JAK2/STAT3 signaling pathway in hepatocellular carcinoma. Translational cancer research, 2023 (PubMed: 37588735) [IF=0.9]

Application: IF/ICC    Species: Human    Sample: SMMC-7721 and MHCC-97H cells

Figure 1 Transfection of SMMC-7721 and MHCC-97H cells with CLDN6-siRNA or NC-siRNA for 48 h. (A,B) Detection of CLDN6 expression by qRT-PCR, ***, P

Application: WB    Species: Human    Sample: SMMC-7721 and MHCC-97H cells

Figure 4 Induction of apoptosis via downregulation of JAK2/STAT3 signaling pathway activation by CLDN6 silencing. Transfection of MMC-7721 and MHCC-97H cells with CLDN6-siRNA or NC-siRNA sequence. (A,B) Attenuation in p-JAK2, p-STAT3, p-JAK2/JAK2, p-STAT3/STAT3, CLDN6 and Bcl-2 expression and enhancement of Bax expression and cleaved caspase-3 levels via CLDN6 silencing (*, P

2). Identification and functional characterization of potential oncofetal targets in human hepatocellular carcinoma. STAR Protocols, 2022 (PubMed: 36595904)

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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