Product: Cytochrome P450 17A1 Antibody
Catalog: AF5210
Description: Rabbit polyclonal antibody to Cytochrome P450 17A1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 57 kDa; 57kD(Calculated).
Uniprot: P05093
RRID: AB_2837696

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
Cytochrome P450 17A1 Antibody detects endogenous levels of total Cytochrome P450 17A1.
RRID:
AB_2837696
Cite Format: Affinity Biosciences Cat# AF5210, RRID:AB_2837696.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

20 lyase; CP17A_HUMAN; CPT7; CYP17; CYP17A1; CYPXVII; Cytochrome P450 17A1; Cytochrome P450 family 17; Cytochrome P450 family 17 subfamily A polypeptide 1; Cytochrome p450 subfamily XVII (steroid 17 alpha hydroxylase) adrenal hyperplasia; Cytochrome p450 XVIIA1; Cytochrome P450-C17; Cytochrome P450c17; OTTHUMP00000020382; P450 C17; P450c17; S17AH; Steroid 17 alpha hydroxylase/17,20 lyase; Steroid 17 alpha monooxygenase; Steroid 17-alpha-hydroxylase/17; Steroid 17-alpha-monooxygenase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty.
Sequence:
MWELVALLLLTLAYLFWPKRRCPGAKYPKSLLSLPLVGSLPFLPRHGHMHNNFFKLQKKYGPIYSVRMGTKTTVIVGHHQLAKEVLIKKGKDFSGRPQMATLDIASNNRKGIAFADSGAHWQLHRRLAMATFALFKDGDQKLEKIICQEISTLCDMLATHNGQSIDISFPVFVAVTNVISLICFNTSYKNGDPELNVIQNYNEGIIDNLSKDSLVDLVPWLKIFPNKTLEKLKSHVKIRNDLLNKILENYKEKFRSDSITNMLDTLMQAKMNSDNGNAGPDQDSELLSDNHILTTIGDIFGAGVETTTSVVKWTLAFLLHNPQVKKKLYEEIDQNVGFSRTPTISDRNRLLLLEATIREVLRLRPVAPMLIPHKANVDSSIGEFAVDKGTEVIINLWALHHNEKEWHQPDQFMPERFLNPAGTQLISPSVSYLPFGAGPRSCIGEILARQELFLIMAWLLQRFDLEVPDDGQLPSLEGIPKVVFLIDSFKVKIKVRQAWREAQAEGST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
90
Rabbit
80
Horse
70
Bovine
67
Sheep
67
Pig
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05093 As Substrate

Site PTM Type Enzyme
Y60 Phosphorylation
T70 Phosphorylation
T72 Phosphorylation
S258 Phosphorylation P17612 (PRKACA)
Y329 Phosphorylation

Research Backgrounds

Function:

A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol (Probable). Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).

PTMs:

Phosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity.

Subcellular Location:

Endoplasmic reticulum membrane. Microsome membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

References

1). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology (PubMed: 32457626) [IF=5.6]

Application: WB    Species: mouse    Sample: LC540 (17b-HSD3) cells

FIGURE 3 | Effects of H10 on 17b-HSD3 in LC540 (17b-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17b-HSD3 mRNA.(D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 mM, while the concentration of 3858858 (positive control) was 0.25 mM.

2). Mechanism of cadmium poisoning on testicular injury in mice. Oncology Letters (PubMed: 31423163) [IF=2.9]

Application: IHC    Species: mouse    Sample: testicular

Figure 3.| Influence of cadmium on testicular tissue protein levels and apoptosis of testicular cells. (A) H&E staining revealed testicular morphology. Immunohistochemical staining demonstrated that the testicular morphology and expression levels of LHR, 17α‑hydroxylase and eNOS were altered in testicular tissues following administration of different concentrations of cadmium. Magnification, x400.

Application: WB    Species: mouse    Sample: testicular

Figure 4.| Influence of cadmium on Leydig cell protein expression levels determined by western blotting. (A) Expression levels of LHR, 17α‑hydroxylase and eNOS were altered in testicular tissues following administration of different concentrations of cadmium.

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