TOMM20 Antibody - #AF5206

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Product: TOMM20 Antibody
Catalog: AF5206
Description: Rabbit polyclonal antibody to TOMM20
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: Q15388
RRID: AB_2837692

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
TOMM20 Antibody detects endogenous levels of total TOMM20.
RRID:
AB_2837692
Cite Format: Affinity Biosciences Cat# AF5206, RRID:AB_2837692.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

KIAA0016; MAS20; MGC117367; Mitochondrial 20 kDa outer membrane protein; Mitochondrial import receptor subunit TOM20 homolog; MOM19; Outer mitochondrial membrane receptor Tom20; TOM20; TOM20_HUMAN; TOMM20; Translocase of outer mitochondrial membrane 20 homolog (yeast); Translocase of outer mitochondrial membrane 20 homolog type II;

Immunogens

Immunogen:
Uniprot:

Q15388(TOM20_HUMAN) >>Visit HPA database.

Gene(ID):
TOMM20(9804)
Description:
Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore
Sequence:
MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
82
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15388 As Substrate

Site PTM Type Enzyme
Ubiquitination
K35 Ubiquitination
S55 Phosphorylation
K56 Acetylation
K56 Ubiquitination
K61 Ubiquitination
K68 Ubiquitination
S135 Phosphorylation
S138 Phosphorylation

Research Backgrounds

Function:

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore (By similarity). Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases.

PTMs:

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Subcellular Location:

Mitochondrion outer membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). Interacts with TOM22. Interacts with APEX1.

Family&Domains:

Belongs to the Tom20 family.

References

1). The role of the apoptosis-related protein BCL-B in the regulation of mitophagy in hepatic stellate cells during the regression of liver fibrosis. EXPERIMENTAL AND MOLECULAR MEDICINE, 2019 (PubMed: 30635551) [IF=9.5]

Application: WB    Species: mouse    Sample: hepatic fibrosis

Fig. 4 |Inhibition of mitophagy aggravates hepatic fibrosis in mice. a H&E staining of liver sections from mice; n = 6 per group; scale bar, 50 μm.b Sirius red staining of collagen in mouse liver sections; n = 6 per group; scale bar, 50 μm. c Representative western blots of TOM20, collagen I and α-SMA. Bar graph represents the mean ± SEM of three different experiments. *P < 0.05; **P < 0.01; and ***P < 0.001 vs. the indicated groups. d Serum levels of ALT and AST in the indicated mice. Bar graph represents the mean ± SEM; n = 6 per group. *P < 0.05 vs. The indicated groups
2). PINK1/Parkin pathway-mediated mitophagy by AS-IV to explore the molecular mechanism of muscle cell damage. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023 (PubMed: 36948131) [IF=6.9]

Application: WB    Species: Rat    Sample: L6 myoblasts

Fig. 3. AS-IV ameliorates mitochondrial damage induced by H2O2 combined with CCCP in L6 myoblasts. (A, B) Effects of AS-IV on intracellular ROS (bar = 100 µm). (C) Effects of AS-IV on ATP activities. (D) Effects of AS-IV on Tom 20 mRNA expression. (E) Effects of AS-IV on Tim 23 mRNA expression. (F) Effects of AS-IV on VDAC1 mRNA expression. (G) Effects of AS-IV on Tom 20 protein expression. (H) Effects of AS-IV on Tim 23 protein expression. (I) Effects of AS-IV on VDAC1 protein expression. ★P 
3). Regulatory mechanism of perinatal nonylphenol exposure on cardiac mitochondrial autophagy and the PINK1/Parkin signaling pathway in male offspring rats. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38367424) [IF=6.7]
4). Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy. Free radical research, 2023 (PubMed: 37897414) [IF=3.6]
5). Mechanism of Baihu Renshen decoction on T2DM rats based on mitochondrial autophagy mediated by PINK1/Parkin. , 2023

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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