Integrin alpha 5 Antibody - #AF5200
| Product: | Integrin alpha 5 Antibody |
| Catalog: | AF5200 |
| Description: | Rabbit polyclonal antibody to Integrin alpha 5 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Horse, Rabbit, Dog |
| Mol.Wt.: | 114kDa,150kDa; 115kD(Calculated). |
| Uniprot: | P08648 |
| RRID: | AB_2837686 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5200, RRID:AB_2837686.
Fold/Unfold
CD49 antigen-like family member E; CD49e; Fibronectin receptor subunit alpha; Fibronectin receptor, alpha subunit; FNRA; Integrin alpha 5 (fibronectin receptor alpha); Integrin alpha-5; Integrin alpha-5 light chain; Integrin alpha-F; Integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITA5_HUMAN; Itga5; Very late activation protein 5, alpha subunit; VLA-5; VLA5; VLA5A;
Immunogens
- P08648 ITA5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P08648 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N84 | N-Glycosylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| N182 | N-Glycosylation | Uniprot | |
| S210 | Phosphorylation | Uniprot | |
| N297 | N-Glycosylation | Uniprot | |
| N307 | N-Glycosylation | Uniprot | |
| N316 | N-Glycosylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| Y487 | Phosphorylation | Uniprot | |
| N609 | N-Glycosylation | Uniprot | |
| N675 | N-Glycosylation | Uniprot | |
| C731 | S-Nitrosylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| N773 | N-Glycosylation | Uniprot | |
| S887 | Phosphorylation | Uniprot | |
| K943 | Ubiquitination | Uniprot | |
| K1042 | Ubiquitination | Uniprot | |
| S1047 | Phosphorylation | Uniprot |
Research Backgrounds
Integrin alpha-5/beta-1 (ITGA5:ITGB1) is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. ITGA5:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1. ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1. ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling. ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling.
(Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human metapneumovirus.
(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human parvovirus B19.
(Microbial infection) In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Proteolytic cleavage by PCSK5 mediates activation of the precursor.
Membrane>Single-pass type I membrane protein. Cell junction>Focal adhesion. Cell surface.
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5 and NISCH. Interacts with RAB21 and COMP. Interacts with CIB1. ITGA5:ITGB1 interacts with CCN3. ITGA5:ITGB1 interacts with FBN1. ITGA5:ITGB1 interacts with IL1B.
(Microbial infection) Integrin ITGA5:ITGB1 interacts with human metapneumovirus fusion protein.
(Microbial infection) Integrin ITGA2:ITGB1 interacts with human parvovirus B19 capsid proteins.
(Microbial infection) Interacts with HIV-1 Tat.
Belongs to the integrin alpha chain family.
Research Fields
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Pertussis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).
· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).
· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).
· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)
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