Product: SOD1 Antibody
Catalog: AF5198
Description: Rabbit polyclonal antibody to SOD1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Pig
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 16 Kd; 16kD(Calculated).
Uniprot: P00441
RRID: AB_2837684

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
SOD1 Antibody detects endogenous levels of total SOD1.
RRID:
AB_2837684
Cite Format: Affinity Biosciences Cat# AF5198, RRID:AB_2837684.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ALS; ALS1; Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Epididymis secretory protein Li 44; HEL S 44; Homodimer; hSod1; Indophenoloxidase A; IPOA; Mn superoxide dismutase; SOD; SOD soluble; SOD1; SOD2; SODC; SODC_HUMAN; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; Superoxide dismutase 1 soluble; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
Sequence:
MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Pig
91
Dog
91
Chicken
82
Xenopus
73
Zebrafish
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00441 As Substrate

Site PTM Type Enzyme
A2 Acetylation
T3 Phosphorylation
K4 Ubiquitination
C7 S-Nitrosylation
K10 Acetylation
K10 Ubiquitination
K24 Acetylation
K24 Ubiquitination
S26 Phosphorylation
K31 Ubiquitination
S35 Phosphorylation
K37 Ubiquitination
T40 Phosphorylation
T59 Phosphorylation
S60 Phosphorylation
S69 Phosphorylation
K71 Acetylation
K71 Ubiquitination
K76 Sumoylation
K76 Ubiquitination
R80 Methylation
T89 Phosphorylation
K92 Ubiquitination
S99 Phosphorylation
S103 Phosphorylation
S106 Phosphorylation
S108 Phosphorylation
K123 Acetylation
K123 Ubiquitination
K129 Ubiquitination
K137 Ubiquitination
R144 Methylation
C147 S-Nitrosylation

Research Backgrounds

Function:

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

PTMs:

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.

The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.

Palmitoylation helps nuclear targeting and decreases catalytic activity.

Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

Subcellular Location:

Cytoplasm. Mitochondrion. Nucleus.
Note: Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not.

Family&Domains:

Belongs to the Cu-Zn superoxide dismutase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

References

1). Chlorogenic acid exerts neuroprotective effect against hypoxia-ischemia brain injury in neonatal rats by activating Sirt1 to regulate the Nrf2-NF-κB signaling pathway. Cell Communication and Signaling (PubMed: 35689269) [IF=8.4]

Application: WB    Species: Rat    Sample: brain tissue

Fig. 2 Chlorogenic acid-induced protection of brain post-hypoxic-ischemic brain injury via the down-regulation of expression of inflammatory and oxidative stress levels. a The IL-1 β level in brain tissue 24 h after HI brain injury measured by Elisa kit. ∗P < 0.05 vs. the sham group. ##P < 0.01 vs. the HI group. n=3. b The MDA level in brain tissue 24 h after HI brain injury by MDA kit. ∗∗∗P < 0.001 vs. the sham group. #P < 0.05 vs. the HI group. n=3. c The CAT level in brain tissue 24 h after HI brain injury by CAT kit. ∗∗∗P < 0.001 vs. the sham group. #P < 0.05 vs. the HI group. n=3. d Western blot detection of the protein levels of iNOS, SOD2/MnSOD, TNF-α, pre-IL-1β and mature- IL-1β 24h after HI injury. e–h Quantification of western blot data of iNOS, SOD2/MnSOD, TNF-α, and mature-IL-1 β. ∗∗P < 0.01 and ∗∗∗P < 0.001 vs. the sham group. #P < 0.05, ##P < 0.01 and ###P < 0.001 vs. the HI group. n = 3

2). Compound Danshen Dripping Pill inhibits doxorubicin or isoproterenol-induced cardiotoxicity. Biomedicine & Pharmacotherapy (PubMed: 34311530) [IF=7.5]

3). Gastroprotective mechanism of modified lvdou gancao decoction on ethanol-induced gastric lesions in mice: Involvement of Nrf-2/HO-1/NF-κB signaling pathway. Frontiers in Pharmacology (PubMed: 36120337) [IF=5.6]

Application: WB    Species: Mouse    Sample: gastric tissues

FIGURE 7 Effect of MLG on some protein levels in ethanol-induced gastric lesions mice. Some representative western blot bands (A). Protein levels of SOD1/GAPDH (B), SOD2/GAPDH (C), iNOS/GAPDH (D), nNOS/GAPDH (E), eNOS/GAPDH (F), COX2/GAPDH (G), p38/GAPDH (H) in mice gastric tissues. SOD1, Superoxide dismutase one or Cu/Zn-superoxide dismutase; SOD2/Mn SOD, superoxide dismutase 2. Control: the group administered zero ethanol; Model: the group administered ethanol intragastrically (13.25 ml/kg BW); MLG-L: low-dose (5 g/kg body weight) MLG-treated group; MLG-M: medium-dose (10 g/kg body weight) MLG-treated group; MLG-H: high-dose (20 g/kg body weight) MLG-treated group; CBP: the group receiving Colloid Bismuth Pectin (57 mg/kg body weight). Each group’s data was expressed as mean ± standard deviation (SD), n = 6. By one-way analysis of variance (ANOVA) test, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 vs. each group. Whole page of western blot can be found in Supplementary Figures S1, S3, S7, S8, S10, S14, S15, respectively.

4). Carnitine palmitoyltransferase 1 (CPT1) alleviates oxidative stress and apoptosis of hippocampal neuron in response to beta-Amyloid peptide fragment Aβ25-35. Bioengineered (PubMed: 34424821) [IF=4.9]

Application: WB    Species: Mice    Sample: Aβ25-35-induced HT22 cells

Figure 3. CPT1C overexpression attenuated oxidative stress in Aβ25-35-induced HT22 cells. Following transfection of Ov-CPT1C or Ov-NC for 24 h, HT22 cells were treated with Aβ25–35 for another 24 h (a) ROS expression was detected using ROS kits. (b) MDA levels and SOD activity were measured using MDA and SOD kits, respectively. (c) The relative mRNA and protein expression of SOD1 and SOD2 in A25-35-induced HT22 cells were measured using RT-qPCR and western blot, respectively. ***P < 0.001 vs. Control group, ##P < 0.01 and ###P < 0.001 vs A25-35 + Ov-NC.

5). DL-3-n-butylphthalide improves ventricular function, and prevents ventricular remodeling and arrhythmias in post-MI rats. Naunyn-Schmiedeberg's Archives of Pharmacology (PubMed: 29602954) [IF=3.6]

Application: WB    Species: rat    Sample: ventricular

Fig. 6 |NBP upregulated PI3k/Akt/Nrf2 signaling pathway and downstream antioxidant enzymes, including HO-1, GSH, SOD1, and SOD2, analyzed by western blot. Data are expressed as means + SD. *P < 0.05 versus Sham group; #P < 0.05 versus MI group

6). Antioxidative effect of Lactobacillus plantarum Y44 on 2,2\'-azobis(2-methylpropionamidine) dihydrochloride (ABAP)-damaged Caco-2 cells. JOURNAL OF DAIRY SCIENCE (PubMed: 31178173) [IF=3.5]

7). LINC00987 Ameliorates COPD by Regulating LPS-Induced Cell Apoptosis, Oxidative Stress, Inflammation and Autophagy Through Let-7b-5p/SIRT1 Axis. International Journal of Chronic Obstructive Pulmonary Disease (PubMed: 33311978) [IF=2.8]

Application: WB    Species: Human    Sample: LPS-mediated 16HBE and BEAS-2B cells

Figure 2 LINC00987 inhibited cell apoptosis, oxidative stress, inflammation and autophagy in LPS-mediated 16HBE and BEAS-2B cells. (A) The transfection efficiency of LINC00987 was detected by RT-qPCR in LPS-induced 16HBE and BEAS-2B cells. (B and C) The effects of LPS treatment and LINC00987 overexpression on LINC00987 and let-7b-5p expression were demonstrated by RT-qPCR. (D) CCK-8 assay was performed to illustrate the impacts between LPS and LINC00987 on cell viability in 16HBE and BEAS-2B cells. (E and F) Caspase3 activity and apoptosis analysis assays were conducted to present the influences between LPS and LINC00987 overexpression on caspase3 activity and cell apoptosis, respectively, in 16HBE and BEAS-2B cells. (G and H) ROS detection kit and SOD activity assays were carried out to uncover the impacts between LPS and enforced LINC00987 expression on oxidative stress in 16HBE and BEAS-2B cells. (I) Western blot assay was employed to determine the impacts of LINC00987 on the protein levels of pro-c3, cleaved-c3, SOD1, SOD2 and SOD3 in LPS-induced 16HBE and BEAS-2B cells. (J and K) ELISA kit assays were employed to exhibit the effects between LPS exposure and LINC00987 on the production of IL-6 and IL-8 in 16HBE and BEAS-2B cells. (L) The effects of LPS treatment and LINC00987 overexpression on the level of IC3-II/IC3-I and the protein expression of ATG5 and P62 were demonstrated by Western blot in 16HBE and BEAS-2B cells. *P<0.05, **P<0.01 and ***P<0.001.

8). Pachymic acid ameliorates pulmonary hypertension by regulating Nrf2-Keap1-ARE pathway. Current Medical Science (PubMed: 34881424) [IF=2.4]

9). Quercetin reverses 5-fluorouracil resistance in colon cancer cells by modulating the NRF2/HO-1 pathway. European Journal of Histochemistry (PubMed: 37548240) [IF=2.0]

10). Oregano Essential Oil Induces SOD1 and GSH Expression through Nrf2 Activation and Alleviates Hydrogen Peroxide-Induced Oxidative Damage in IPEC-J2 Cells. Oxidative Medicine and Cellular Longevity (PubMed: 28105249)

Application: WB    Species: pig    Sample: IPEC-J2 Cells

Figure 3: Effects of oregano essential oil (OEO) on g-glutamylcysteine ligase (GCLM, GCLC), and Cu/Zn-superoxide dismutase (SOD1) expression in IPEC-J2 cells. (a–d) IPEC-J2 cells were incubated with OEO 10

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