BMP7 Antibody - #AF5193

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Product: BMP7 Antibody
Catalog: AF5193
Description: Rabbit polyclonal antibody to BMP7
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Dog, Chicken
Mol.Wt.: 49 kDa; 49kD(Calculated).
Uniprot: P18075
RRID: AB_2837679

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(92%), Sheep(92%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
BMP7 Antibody detects endogenous levels of total BMP7.
RRID:
AB_2837679
Cite Format: Affinity Biosciences Cat# AF5193, RRID:AB_2837679.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMP 7; BMP-7; Bmp7; BMP7_HUMAN; Bone morphogenetic protein 7; Bone morphogenic protein 7; Eptotermin alfa; OP 1; OP-1; OP1; Osteogenic protein 1;

Immunogens

Immunogen:
Uniprot:

P18075(BMP7_HUMAN) >>Visit HPA database.

Gene(ID):
BMP7(655)
Expression:
P18075 BMP7_HUMAN:

Expressed in the kidney and bladder. Lower levels seen in the brain.

Description:
Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.
Sequence:
MHVRSLRAAAPHSFVALWAPLFLLRSALADFSLDNEVHSSFIHRRLRSQERREMQREILSILGLPHRPRPHLQGKHNSAPMFMLDLYNAMAVEEGGGPGGQGFSYPYKAVFSTQGPPLASLQDSHFLTDADMVMSFVNLVEHDKEFFHPRYHHREFRFDLSKIPEGEAVTAAEFRIYKDYIRERFDNETFRISVYQVLQEHLGRESDLFLLDSRTLWASEEGWLVFDITATSNHWVVNPRHNLGLQLSVETLDGQSINPKLAGLIGRHGPQNKQPFMVAFFKATEVHFRSIRSTGSKQRSQNRSKTPKNQEALRMANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIAPEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHFINPETVPKPCCAPTQLNAISVLYFDDSSNVILKKYRNMVVRACGCH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Chicken
100
Horse
92
Sheep
92
Xenopus
78
Pig
0
Bovine
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18075 As Substrate

Site PTM Type Enzyme
K162 Ubiquitination
T306 Phosphorylation
S322 Phosphorylation
S323 Phosphorylation

Research Backgrounds

Function:

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

PTMs:

Several N-termini starting at positions 293, 300, 315 and 316 have been identified by direct sequencing resulting in secretion of different mature forms.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the kidney and bladder. Lower levels seen in the brain.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with TWSG1 (By similarity). Interacts with FBN1 (via N-terminal domain) and FBN2.

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). TNIK drives castration-resistant prostate cancer via phosphorylating EGFR. iScience, 2024 (PubMed: 38226156) [IF=5.8]

Application: IHC    Species: Mouse    Sample:

Figure 6 Targeting TNIK suppresses CRPC tumor progression in vivo (A) C4-2 cells were implanted subcutaneously in male BALB/c mice. When tumors became palpable, mice administered daily by oral gavage either with vehicle (10% DMSO in PBS) or NCB0846 (80 mg/kg of body weight) for 10 days (n = 4 mice for each treatment). Tumor volumes were measured with calipers. (B) Tumor size of xenografts of the above represented the growth of tumor over 10 days (n = 4) in athymic nude mice (p < 0.001). Data are shown as mean ± SD. (C) Tumor weight of the control mice tumors and NCB-0846-treated mice tumors (p < 0.001). Data are shown as mean ± SD. (D) Body weight of nude mice after implantation of control or C4-2 xenografts and treatment with vehicle or NCB-0846 for 4 weeks. (E) Quantitation of Ki-67, TNIK, p-EGFR, β-catenin, vimentin, E-cadherin, BMP6, and BMP7 expressions in C4-2 xenograft tumors from each group; specimens were got at 10 days posttreatment. Scale bars: 500 μm. The IHC was scored according to number of cells expressing the indicated proteins, and statistical analysis was performed (non-parametric Kruskal-Wallis test) in order to determine significance. Data are shown as mean ± SD.
2). Lipopolysaccharide-preconditioned allogeneic adipose-derived stem cells improve erectile function in a rat model of bilateral cavernous nerve injury. Basic and Clinical Andrology, 2022 (PubMed: 35337262) [IF=2.4]

Application: WB    Species: Rat    Sample: penile tissues

Fig. 10 L-ADSCs prevented penile fibrosis. A, B Western blot analysis of fibronectin, collagen I, HGF and TGF-β1 protein expressions in penile tissues in the sham, PBS, ADSCs and L-ADSCs groups. Error bars: mean ± standard deviation from n = 6 animals per group. *p < 0.05, ***p < 0.001. #p < 0.05. ADSCs: adipose tissue-derived stem cells; L-ADSCs: lipopolysaccharide-preconditioned adipose tissue-derived stem cells; TGF-β1: transforming growth factor-beta1; HGF: hepatocyte growth factor

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