Product: Cathepsin D Antibody
Catalog: AF5191
Description: Rabbit polyclonal antibody to Cathepsin D
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 44 kDa, 25 kDa; 45kD(Calculated).
Uniprot: P07339
RRID: AB_2837677

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(82%), Sheep(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
Cathepsin D Antibody detects endogenous levels of total Cathepsin D.
RRID:
AB_2837677
Cite Format: Affinity Biosciences Cat# AF5191, RRID:AB_2837677.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CatD; CATD_HUMAN; Cathepsin D; Cathepsin D heavy chain; CD; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; ctsd; Epididymis secretory sperm binding protein Li 130P; HEL S 130P; Lysosomal aspartyl peptidase; Lysosomal aspartyl protease; MGC2311;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P07339 CATD_HUMAN:

Expressed in the aorta extracellular space (at protein level) (PubMed:20551380). Expressed in liver (at protein level) (PubMed:1426530).

Description:
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Sequence:
MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
91
Dog
91
Pig
82
Bovine
82
Xenopus
55
Zebrafish
55
Chicken
45
Horse
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P07339 As Substrate

Site PTM Type Enzyme
K28 Ubiquitination
S37 Phosphorylation
S42 Phosphorylation
K49 Ubiquitination
K54 Ubiquitination
T63 O-Glycosylation
K127 Ubiquitination
N134 N-Glycosylation
K184 Ubiquitination
T250 Phosphorylation
S252 Phosphorylation
K253 Ubiquitination
N263 N-Glycosylation
K313 Ubiquitination
Y325 Phosphorylation
K331 Ubiquitination
S333 Phosphorylation
T334 Phosphorylation
T339 Phosphorylation
K341 Ubiquitination
Y347 Phosphorylation
K348 Ubiquitination
Y354 Phosphorylation
K357 Ubiquitination
Y394 Phosphorylation

Research Backgrounds

Function:

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

PTMs:

N- and O-glycosylated.

Undergoes proteolytic cleavage and activation by ADAM30.

As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.

Subcellular Location:

Lysosome. Melanosome. Secreted>Extracellular space.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).

Subunit Structure:

Consists of a light chain and a heavy chain. Interacts with ADAM30; this leads to activation of CTSD. Interacts with GRN; stabilizes CTSD; increases its proteolytic activity (By similarity).

Family&Domains:

Belongs to the peptidase A1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

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