Cathepsin D Antibody - #AF5191
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5191, RRID:AB_2837677.
Fold/Unfold
CatD; CATD_HUMAN; Cathepsin D; Cathepsin D heavy chain; CD; Ceroid lipofuscinosis neuronal 10; CLN10; CPSD; ctsd; Epididymis secretory sperm binding protein Li 130P; HEL S 130P; Lysosomal aspartyl peptidase; Lysosomal aspartyl protease; MGC2311;
Immunogens
Expressed in the aorta extracellular space (at protein level) (PubMed:20551380). Expressed in liver (at protein level) (PubMed:1426530).
- P07339 CATD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVEDLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPCQSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNLMQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQVEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVSTLPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGRYYTVFDRDNNRVGFAEAARL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P07339 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K28 | Ubiquitination | Uniprot | |
| S37 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| T63 | O-Glycosylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| N134 | N-Glycosylation | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| T250 | Phosphorylation | Uniprot | |
| S252 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| N263 | N-Glycosylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T334 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| Y354 | Phosphorylation | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| Y394 | Phosphorylation | Uniprot |
Research Backgrounds
Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
N- and O-glycosylated.
Undergoes proteolytic cleavage and activation by ADAM30.
As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified. An additional form starting at Ala-168 has also been identified.
Lysosome. Melanosome. Secreted>Extracellular space.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Expressed in the aorta extracellular space (at protein level). Expressed in liver (at protein level).
Consists of a light chain and a heavy chain. Interacts with ADAM30; this leads to activation of CTSD. Interacts with GRN; stabilizes CTSD; increases its proteolytic activity (By similarity).
Belongs to the peptidase A1 family.
Research Fields
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
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