P Glycoprotein 1(MDR1) Antibody - #AF5185
Product: | P Glycoprotein 1(MDR1) Antibody |
Catalog: | AF5185 |
Description: | Rabbit polyclonal antibody to P Glycoprotein 1(MDR1) |
Application: | WB IHC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Horse, Rabbit, Dog, Chicken, Xenopus |
Mol.Wt.: | 141 kDa; 141kD(Calculated). |
Uniprot: | P08183 |
RRID: | AB_2837671 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5185, RRID:AB_2837671.
Fold/Unfold
ABC20; ABCB1; ATP binding cassette, sub family B (MDR/TAP), member 1; ATP-binding cassette sub-family B member 1; CD243; CLCS; Colchicin sensitivity; Doxorubicin resistance; GP170; MDR1; MDR1_HUMAN; Multidrug resistance 1; Multidrug resistance protein 1; P glycoprotein 1; P gp; P-glycoprotein 1; PGY1;
Immunogens
- P08183 MDR1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P08183 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S23 | Phosphorylation | Uniprot | |
T263 | Phosphorylation | Uniprot | |
K271 | Ubiquitination | Uniprot | |
K279 | Ubiquitination | Uniprot | |
K367 | Ubiquitination | Uniprot | |
K372 | Ubiquitination | Uniprot | |
K380 | Ubiquitination | Uniprot | |
K384 | Acetylation | Uniprot | |
K389 | Ubiquitination | Uniprot | |
K405 | Sumoylation | Uniprot | |
K408 | Sumoylation | Uniprot | |
K411 | Ubiquitination | Uniprot | |
T422 | Phosphorylation | Uniprot | |
T496 | Phosphorylation | Uniprot | |
K515 | Ubiquitination | Uniprot | |
K519 | Ubiquitination | Uniprot | |
T522 | Phosphorylation | Uniprot | |
K536 | Ubiquitination | Uniprot | |
K617 | Ubiquitination | Uniprot | |
K619 | Ubiquitination | Uniprot | |
S644 | Phosphorylation | Uniprot | |
S658 | Phosphorylation | Uniprot | |
S660 | Phosphorylation | Uniprot | |
S661 | Phosphorylation | P17252 (PRKCA) | Uniprot |
S667 | Phosphorylation | P17612 (PRKACA) , P17252 (PRKCA) | Uniprot |
S671 | Phosphorylation | P17252 (PRKCA) , P17612 (PRKACA) | Uniprot |
S675 | Phosphorylation | Uniprot | |
S683 | Phosphorylation | P17612 (PRKACA) | Uniprot |
T684 | Phosphorylation | Uniprot | |
S696 | Phosphorylation | Uniprot | |
K826 | Ubiquitination | Uniprot | |
K915 | Ubiquitination | Uniprot | |
T1015 | Phosphorylation | Uniprot | |
T1023 | Phosphorylation | Uniprot | |
T1030 | Phosphorylation | Uniprot | |
K1062 | Ubiquitination | Uniprot | |
Y1087 | Phosphorylation | Uniprot | |
K1093 | Ubiquitination | Uniprot | |
K1099 | Ubiquitination | Uniprot | |
K1102 | Methylation | Uniprot | |
K1172 | Ubiquitination | Uniprot | |
K1181 | Ubiquitination | Uniprot | |
K1212 | Ubiquitination | Uniprot | |
Y1267 | Phosphorylation | Uniprot | |
S1269 | Phosphorylation | Uniprot |
Research Backgrounds
Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins. Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Cell membrane>Multi-pass membrane protein. Apical cell membrane.
Expressed in liver, kidney, small intestine and brain.
Interacts with PSMB5. Finds in a complex with ABCB1, TFPI2 and PPP2R3C; leading to the dephosphorylation of ABCB1.
Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Research Fields
· Environmental Information Processing > Membrane transport > ABC transporters.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
References
Application: WB Species: Human Sample: HepG2 cells
Application: WB Species: human Sample: EOC ascites cells
Application: IF/ICC Species: human Sample:
Application: WB Species: Mouse Sample:
Application: WB Species: Human Sample: A549 and A549/Taxol cells
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