BMP4 Antibody | Affinity Biosciences

WB
IHC
Cites

1/4

Western blot analysis of BMP4 expression in 293 cells.

AF5175 at 1/100 staining Human prostate cancer by IHC-P.

FIGURE 2 Effects of a high-glucose microenvironment on mineralization of DPCs.

Figure 2 Effects of TMF on chondrocyte hypertrophy in vitro.

Product:	BMP4 Antibody
Catalog:	AF5175
Description:	Rabbit polyclonal antibody to BMP4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	46 kDa; 47kD(Calculated).
Uniprot:	P12644
RRID:	AB_2837661

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(93%), Sheep(93%), Rabbit(86%), Dog(93%)

Clonality:

Polyclonal

Specificity:

BMP4 Antibody detects endogenous levels of total BMP4.

RRID:

AB_2837661
Cite Format: Affinity Biosciences Cat# AF5175, RRID:AB_2837661.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

zgc:100779; BMP 2B; BMP 4; BMP-2B; BMP-4; BMP2B; BMP2B1; BMP4; BMP4_HUMAN; Bone morphogenetic protein 2B; Bone morphogenetic protein 4; DVR4; MCOPS6; MGC100779; OFC11; zbmp-4; ZYME;

Immunogens

Immunogen:

Uniprot:

P12644(BMP4_HUMAN) >>Visit HPA database.

Gene(ID):

BMP4(652)

Expression:

P12644 BMP4_HUMAN:

Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

Description:

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction

Sequence:

P12644 BMP4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRRPQPSKSAVIPDYMRDLYRLQSGEEEEEQIHSTGLEYPERPASRANTVRSFHHEEHLENIPGTSENSAFRFLFNLSSIPENEVISSAELRLFREQVDQGPDWERGFHRINIYEVMKPPAEVVPGHLITRLLDTRLVHHNVTRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGNWAQLRPLLVTFGHDGRGHALTRRRRAKRSPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Rabbit

Chicken

Pig

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12644 As Substrate

P12644

Site	PTM Type	Source
Y82	Phosphorylation	Uniprot
S91	Phosphorylation	Uniprot
T116	Phosphorylation	Uniprot
S119	Phosphorylation	Uniprot
K185	Ubiquitination	Uniprot
K228	Ubiquitination	Uniprot

Research Backgrounds

P12644_BMP4_HUMAN

Function:

Induces cartilage and bone formation. Also acts in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction (By similarity).

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.

Subunit Structure:

Homodimer; disulfide-linked (By similarity). Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with SOSTDC1. Interacts with FBN1 (via N-terminal domain) and FBN2.

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)

References

1). Effect of LncRNA-MALAT1 on mineralization of dental pulp cells in a high-glucose microenvironment. Frontiers in Cell and Developmental Biology, 2022 (PubMed: 36035997) [IF=5.5]

Application: WB Species: Human Sample:

FIGURE 2 Effects of a high-glucose microenvironment on mineralization of DPCs. (A,C) NC group 7 /14 days after induced mineralization stained by alizarin red; (B,D) high-glucose group 7 /14 days after induced mineralization stained by alizarin red; (E) semi-quantitative analysis of alizarin red staining; (F) expression of mineralization-related factors was detected by qRT-PCR after 7 days of DPCs which were cultured in a high-glucose microenvironment; (G) expression of mineralization-related factors was detected by qRT-PCR after 14 days; (H) expression results of mineralization-related factors detected by Western blot after 7 /14 days of DPC induction in a high-glucose microenvironment; (I,J) density ratio of target proteins to GAPDH on 7 days and 14 days (n = 3, scale bar = 500 μm # p < 0.05, *p < 0.01).

2). TMF suppresses chondrocyte hypertrophy in osteoarthritic cartilage by mediating the FOXO3a/BMPER pathway. Experimental and therapeutic medicine, 2024 (PubMed: 38800044) [IF=2.7]

Application: WB Species: Human Sample: C28/I2 cells

Figure 2 Effects of TMF on chondrocyte hypertrophy in vitro. Protein expression levels of (A and B) Col X, (A and C) Runx2, (A and D) BMPER, (A and E) BMP4 and (A and F) p-Smad1/Smad1. Immunofluorescent intensity of (G) Col X and (H) Runx2. *P

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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BMP4 Antibody - #AF5175