PAI 2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/5

Western blot analysis of extracts from various samples, using PAI 2 Antibody.

AF5174 at 1/100 staining human Appendix tissue sections by IHC-P.

AF5174 at 1/100 staining Mouse heart tissue by IHC-P.

AF5174 at 1/100 staining Rat kidney tissue by IHC-P.

AF5174 staining Hela by IF/ICC.

Product:	PAI 2 Antibody
Catalog:	AF5174
Description:	Rabbit polyclonal antibody to PAI 2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Rabbit, Dog
Mol.Wt.:	46 kDa; 47kD(Calculated).
Uniprot:	P05120
RRID:	AB_2837660

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(85%), Rabbit(85%), Dog(85%)

Clonality:

Polyclonal

Specificity:

PAI 2 Antibody detects endogenous levels of total PAI 2.

RRID:

AB_2837660
Cite Format: Affinity Biosciences Cat# AF5174, RRID:AB_2837660.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HsT1201; Monocyte Arg serpin; Monocyte Arg-serpin; Monocyte Arginine-serpin; Monocyte-derived plasminogen activator inhibitor; PAI; PAI-2; PAI2; PAI2_HUMAN; Placental plasminogen activator inhibitor; PLANH2; Plasminogen activator inhibitor 2; plasminogen activator inhibitor, type 2; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Serpinb2; Urokinase inhibitor;

Immunogens

Immunogen:

Uniprot:

P05120(PAI2_HUMAN) >>Visit HPA database.

Gene(ID):

SERPINB2(5055)

Description:

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-2.

Sequence:

P05120 PAI2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEDLCVANTLFALNLFKHLAKASPTQNLFLSPWSISSTMAMVYMGSRGSTEDQMAKVLQFNEVGANAVTPMTPENFTSCGFMQQIQKGSYPDAILQAQAADKIHSSFRSLSSAINASTGNYLLESVNKLFGEKSASFREEYIRLCQKYYSSEPQAVDFLECAEEARKKINSWVKTQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRVNSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIADVSTGLELLESEITYDKLNKWTSKDKMAEDEVEVYIPQFKLEEHYELRSILRSMGMEDAFNKGRANFSGMSERNDLFLSEVFHQAMVDVNEEGTEAAAGTGGVMTGRTGHGGPQFVADHPFLFLIMHKITNCILFFGRFSSP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Dog

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P05120 As Substrate

P05120

Site	PTM Type	Source
T69	Phosphorylation	Uniprot
T72	Phosphorylation	Uniprot
S136	Phosphorylation	Uniprot
S171	Phosphorylation	Uniprot
T175	Phosphorylation	Uniprot
T177	Phosphorylation	Uniprot
S296	Phosphorylation	Uniprot

Research Backgrounds

P05120_PAI2_HUMAN

Function:

Inhibits urokinase-type plasminogen activator. The monocyte derived PAI-2 is distinct from the endothelial cell-derived PAI-1.

PTMs:

The signal sequence is not cleaved.

Subcellular Location:

Cytoplasm. Secreted>Extracellular space.

Subunit Structure:

Interacts with PSMB1.

Family&Domains:

Belongs to the serpin family. Ov-serpin subfamily.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Chelerythrine chloride inhibits the progression of colorectal cancer by targeting cancer-associated fibroblasts through intervention with WNT10B/β-catenin and TGFβ2/Smad2/3 axis. Phytotherapy research : PTR, 2023 (PubMed: 37402476) [IF=7.2]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PAI 2 Antibody - #AF5174