BBC3 Antibody - #AF5173

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Product: BBC3 Antibody
Catalog: AF5173
Description: Rabbit polyclonal antibody to BBC3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Dog
Mol.Wt.: 20 kDa; 21kD(Calculated).
Uniprot: Q9BXH1
RRID: AB_2837659

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BBC3 Antibody detects endogenous levels of total BBC3.
RRID:
AB_2837659
Cite Format: Affinity Biosciences Cat# AF5173, RRID:AB_2837659.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BBC 3; Bbc3; BBC3_HUMAN; BCL 2 binding component 3; Bcl-2-binding component 3; BCL2 binding component 3; JFY 1; JFY-1; JFY1; p53 up regulated modulator of apoptosis; p53 up-regulated modulator of apoptosis; p53 Upregulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1;

Immunogens

Immunogen:

A synthesized peptide derived from human BBC3(PUMA), corresponding to a region within the internal amino acids.

Uniprot:

Q9BXH1(BBC3_HUMAN) >>Visit HPA database.

Gene(ID):
BBC3(27113)
Expression:
Q9BXH1 BBC3_HUMAN:

Ubiquitously expressed.

Description:
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis
Sequence:
MARARQEGSSPEPVEGLARDGPRPFPLGRLVPSAVSCGLCEPGLAAAPAAPTLLPAAYLCAPTAPPAVTAALGGSRWPGGPRSRPRGPRPDGPQPSLSLAEQHLESPVPSAPGALAGGPTQAAPGVRGEEEQWAREIGAQLRRMADDLNAQYERRRQEEQQRHRPSPWRVLYNLIMGLLPLPRGHRAPEMEPN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Zebrafish
56
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXH1 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation O14920 (IKBKB) , O15111 (CHUK)
Y58 Phosphorylation P04626 (ERBB2)
S96 Phosphorylation
S106 Phosphorylation
Y152 Phosphorylation P04626 (ERBB2)
S166 Phosphorylation
Y172 Phosphorylation P04626 (ERBB2)

Research Backgrounds

Function:

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.

Subcellular Location:

Mitochondrion.
Note: Localized to the mitochondria in order to induce cytochrome c release.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with MCL1 and BCL2A1 (By similarity). Interacts (via BH3 domain) with BCL2. Interacts with BCL2L1/BCL-XL. Interacts (via BH3 domain) with NOL3/ARC (via CARD domain); this interaction prevents BBC3 association with BCL2 and results in CASP8 activation (By similarity).

Family&Domains:

The BH3 motif is intrinsically disordered.

Belongs to the Bcl-2 family.

Research Fields

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

References

1). Inhibition of REST Suppresses Proliferation and Migration in Glioblastoma Cells. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2016 (PubMed: 27153061) [IF=5.6]

Application: WB    Species: human    Sample:

2). Investigation of the inhibition effect of 1,2,3,4,6-pentagalloylb-D-glucose on gastric cancer cells based on a network pharmacology approach and experimental validation. Frontiers in Oncology, 2022 (PubMed: 35992839) [IF=4.7]
3). The X box binding protein 1/C/EBP homologous protein pathway induces apoptosis of endothelial cells under hyperglycemia. Experimental and Therapeutic Medicine, 2022 (PubMed: 35720621) [IF=2.7]

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