AQP5 Antibody - #AF5169

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Product: AQP5 Antibody
Catalog: AF5169
Description: Rabbit polyclonal antibody to AQP5
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 28 kDa; 28kD(Calculated).
Uniprot: P55064
RRID: AB_2837655

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(88%), Sheep(88%), Dog(94%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
AQP5 Antibody detects endogenous levels of total AQP5.
RRID:
AB_2837655
Cite Format: Affinity Biosciences Cat# AF5169, RRID:AB_2837655.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AQP 5; AQP-5; AQP5; AQP5_HUMAN; Aquaporin 5; Aquaporin-5;

Immunogens

Immunogen:
Uniprot:

P55064(AQP5_HUMAN) >>Visit HPA database.

Gene(ID):
AQP5(362)
Expression:
P55064 AQP5_HUMAN:

Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level).

Description:
Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions.
Sequence:
MKKEVCSVAFLKAVFAEFLATLIFVFFGLGSALKWPSALPTILQIALAFGLAIGTLAQALGPVSGGHINPAITLALLVGNQISLLRAFFYVAAQLVGAIAGAGILYGVAPLNARGNLAVNALNNNTTQGQAMVVELILTFQLALCIFASTDSRRTSPVGSPALSIGLSVTLGHLVGIYFTGCSMNPARSFGPAVVMNRFSPAHWVFWVGPIVGAVLAAILYFYLLFPNSLSLSERVAIIKGTYEPDEDWEEQREERKKTMELTTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
94
Bovine
88
Sheep
88
Chicken
88
Xenopus
46
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55064 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S156 Phosphorylation P17612 (PRKACA)
Y243 Phosphorylation
K258 Acetylation
T259 Phosphorylation

Research Backgrounds

Function:

Forms a water-specific channel. Plays an important role in fluid secretion in salivary glands (By similarity). Required for TRPV4 activation by hypotonicity. Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells. Seems to play a redundant role in water transport in the eye, lung and in sweat glands (By similarity).

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein. Cytoplasmic vesicle membrane>Multi-pass membrane protein.
Note: Hypotonicity increases location at the cell membrane. Phosphorylation decreases location at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level).

Subunit Structure:

Homotetramer. Interacts with TRPV4; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity.

Family&Domains:

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Belongs to the MIP/aquaporin (TC 1.A.8) family.

Research Fields

· Organismal Systems > Digestive system > Salivary secretion.

References

1). Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY, 2022 (PubMed: 35688257) [IF=5.4]
2). Combination of pseudoephedrine and emodin ameliorates LPS-induced acute lung injury by regulating macrophage M1/M2 polarization through the VIP/cAMP/PKA pathway. Chinese Medicine, 2022 (PubMed: 35123524) [IF=4.9]

Application: WB    Species: Rat    Sample: lung tissues

Fig. 1 Effect of Pseudoephedrine + emodin on pathological changes of lung tissues (×200), expression of AQP‐1, AQP‐5 in LPS‐induced ALI rats. A The chemical structure of Pseudoephedrine and Emodin. B Stained with hematoxylin and eosin. C The lung injury score was determined following a five-point scale from 0 to 4 as follows: 0, l, 2, 3, and 4 represent no damage, mild damage, moderate damage, severe damage, and very severe damage, respectively. Representative histological sections of the lungs were stained with hematoxylin and eosin (H & E staining, magnification ×200). D–F Western blot analysis was performed to detect AQP‐1 and AQP‐5 protein expression. K The dry/wet weight ratio and lung weight/body weight ratio were used to reflect the pulmonary edema. All data are expressed as mean ± S.D. (n = 5). ##p < 0.01, ###p < 0.001 vs. control group. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS alone group. +p < 0.05, +++p < 0.001 vs. combined treatment group (5 + 20 mg/kg)
3). Inhibition of Pre-B Cell Colony Enhancing Factor Reduces Lung Injury in Rats Receiving Cardiopulmonary Bypass. Drug Design Development and Therapy, 2021 (PubMed: 33442236) [IF=4.8]

Application: WB    Species: Rat    Sample: lung tissues

Figure 4 Adenovirus-encoding sh-PBEF reduced the increase of α-subunit of PBEF, SP, AQP1, AQP5, and ENaC in lung tissue. (A) Representative blots; (B) Relative expression of PBEF and SP; (C) Relative expression of AQP1, AQP5, and ENaC; Original blots were shown in Supplemental figure 1; vs control, *P<0.05; vs LPS, #P<0.05; vs CPB+Blank, @P<0.05.
4). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 3. Aquaporin 5 (AQP5) and Na-K-Cl cotransporter 1 (NKCC1) protein expression levels in sweat gland cells extracted from patients with primary palmar hyperhidrosis treated with blank control or transfected with overexpression negative control (OE NC) or integrin β6 (ITGB6) OE. Western blot images (A) and quantitative analyses (B) (ITGB6 OE compared to control, AQP5: Tukey’s honest significant difference (HSD), p = 0.055; NKCC1: Tukey’s HSD, p = 0.190). The data are presented as mean ± 95% confidence interval (95% CI)
5). Evaluation of the Content of Aquaporin-5 and Epithelial Sodium Channel in the Lungs of Rats during the Development of Toxic Pulmonary Edema Caused by Intoxication with Acylating Pulmonotoxicants. Bulletin of Experimental Biology and Medicine, 2022 (PubMed: 36210422) [IF=0.7]

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