Product: BMP2 Antibody
Catalog: AF5163
Description: Rabbit polyclonal antibody to BMP2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 14~20kD(monomer), 30~40kD(dimer), 44~60kD(precursor); 45kD(Calculated).
Uniprot: P12643
RRID: AB_2837649

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Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
BMP2 Antibody detects endogenous levels of total BMP2.
RRID:
AB_2837649
Cite Format: Affinity Biosciences Cat# AF5163, RRID:AB_2837649.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BMP 2; BMP 2A; BMP2; BMP2A; Bone morphogenetic protein 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P12643 BMP2_HUMAN:

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Description:
Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
Sequence:
MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTRLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Chicken
100
Bovine
91
Sheep
91
Rabbit
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12643 As Substrate

Site PTM Type Enzyme
T181 Phosphorylation
S184 Phosphorylation
K278 Ubiquitination
N338 N-Glycosylation
K383 Ubiquitination

Research Backgrounds

Function:

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with SOSTDC1. Interacts with GREM2, RGMA, RGMB and RGMC. Interacts with ASPN (By similarity). Interacts with MAFP5 (By similarity). Interacts with FBN1 (via N-terminal domain) and FBN2.

Family&Domains:

Belongs to the TGF-beta family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma.   (View pathway)

References

1). Mechanosensitive Piezo1 is crucial for periosteal stem cell-mediated fracture healing. International Journal of Biological Sciences (PubMed: 35844802) [IF=9.2]

2). Osteogenic potential evaluation of biotin combined with magnesium-doped hydroxyapatite sustained-release film. Materials science & engineering-C, Materials for biological applications (PubMed: 35581076) [IF=7.9]

3). Magnesium-enriched microenvironment promotes odontogenic differentiation in human dental pulp stem cells by activating ERK/BMP2/Smads signaling. Stem Cell Research & Therapy (PubMed: 31823825) [IF=7.5]

Application: WB    Species: Human    Sample: DPSCs

Fig. 6 The ERK and BMP2 signaling pathway is activated by high extracellular Mg2+ in DPSCs during odontogenic differentiation. a ERK phosphorylation was significantly enhanced in DPSCs treated with 1 mM, 5 mM, and 10 mM Mg2+ compared with the 0 mM Mg2+ group, but p38 and JNK phosphorylation amounts were unchanged. b ERK phosphorylation was reduced by 2-APB. c Consistently, the protein levels of BMP2, BMPR1, and phosphorylated Smad1/5/9 were significantly increased in DPSCs exposed to high extracellular Mg2+. d BMP2, BMPR1, and phosphorylated Smad1/5/9 protein amounts were decreased by 2-APB

4). Panax notoginseng Suppresses Bone Morphogenetic Protein-2 Expression in EA.hy926 Endothelial Cells by Inhibiting the Noncanonical NF-κB and Wnt/β-Catenin Signaling Pathways. Plants (PubMed: 36501304) [IF=4.5]

5). miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3. Stem Cells International (PubMed: 34326879) [IF=4.3]

Application: WB    Species: Mice    Sample: BMSCs

Figure 3 miR-129-5p promoted osteoblast differentiation of BMSCs. BMSCs were transduced with a lentiviral vector containing miR-129-5p mimic, miR-129-5p inhibitor, or negative control (NC) lentivirus, respectively. 48 hours after transduction, cells were cultured in osteoblast induction medium. ALP staining was performed at days 7 and 14 following induction (a); corresponding ALP absorbance index was calculated (b). ARS staining of BMSCs was performed at days 14 and 21 following induction (c); corresponding ARS absorbance index was calculated (d). mRNA (e) and protein (f) levels of osteoblast-related genes Runx2, Bmp2, and OCN were detected by quantitative RT-PCR and western blot, respectively. β-Actin was used as an internal control. All data were expressed as means ± SD. ∗p < 0.05 and ∗∗p < 0.01.

6). ITE promotes hypoxia-induced transdifferentiation of human pulmonary arterial endothelial cells possibly by activating transforming growth factor-β/Smads and MAPK/ERK pathways. JOURNAL OF CELLULAR BIOCHEMISTRY (PubMed: 31297875) [IF=4.0]

Application: WB    Species: human    Sample: HPAECs

FIGURE 6 |Effects of ITE on the expression of TGF‐β1, BMP2 and BMP9 in HPAECs induced by hypoxia. HPAECs were cultured under the conditions of normoxia, normoxia + ITE, hypoxia and hypoxia + ITE, respectively. *P < 0.05, **P < 0.01, and ***P < 0.001. BMP, bone morphogenetic protein; HPAEC, human pulmonary arterial endothelial cell; ITE, 2‐(1′H‐indole‐3′‐carbonyl)‐thiazole‐4‐carboxylicacid methyl ester; TGF‐β, transforming growth factor‐β

7). P53 negatively regulates the osteogenic differentiation in jaw bone marrow MSCs derived from diabetic osteoporosis. Heliyon (PubMed: 37096002) [IF=4.0]

Application: WB    Species: Human    Sample: hJBMMSCs

Fig. 2. Comparison of osteogenic differentiation ability between NC and DOP(diabetic osteoporosis) hJBMMSCs. A. The ALP staining area was smaller and the color was lighter in DOP group than NC group on the 10th day (Mean ± SD, n = 6). B. The ALP activity was lower in DOP group than NC group after 7 days and 14 days of osteogenic induction (Mean ± SD, n = 6). C. After osteogenic induction, ARS (alizarin red S) staining on the 21st day showed that the number of mineralized nodules in DOP group was significantly less than that in NC group (Mean ± SD, n = 6). D. semiquantitative analysis showed that the OD (optical density) value of DOP group was significantly lower than that of NC group (Mean ± SD, n = 6). E. After osteogenic induction, the mRNA expressions of RUNX2, BMP2,ALP, OCN, SP7 and DLX5 in DOP group were significantly lower than those in NC group (Mean ± SD, n = 3). F. The protein expressions of RUNX2, BMP2, ALP and DLX5 in DOP group were significantly lower than those in NC group (Mean ± SD, n = 3). **P < 0.01,***P < 0.001.

8). Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling. Toxicology and Applied Pharmacology (PubMed: 33171191) [IF=3.8]

9). Melatonin alleviates hydrogen peroxide induced oxidative damage in MC3T3-E1 cells and promotes osteogenesis by activating SIRT1. FREE RADICAL RESEARCH (PubMed: 35109721) [IF=3.3]

10). Arbutin ameliorates glucocorticoid-induced osteoporosis through activating autophagy in osteoblasts. EXPERIMENTAL BIOLOGY AND MEDICINE (PubMed: 33757338) [IF=3.2]

Application: WB    Species: mice    Sample: MC3T3-E1 cells

Figure 2. Effect of arbutin on biochemical markers of bone turnover. (a, b) Serum levels of bone resorption markers: CTX-1 and TRAP. (c, d) Serum levels of bone formation markers: osteocalcin and ALP. (e, f) Femoral Runx2 and BMP2 levels detected by Western blot analysis, and the relative expression to GAPDH was quantified. Data presented as mean  SD (n ¼ 6/group). *P<0.05, **P<0. 01, ***P<0.001, ****P<0.0001. (A color version of this figure is available in the online journal.) CTX-1: cross-linked C-telopeptide of type I collagen; TRAP: tartrate-resistant acid phosphatase; ALP: alkaline phosphatase; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

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