Integrin alpha E Antibody - #AF5155

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Product: Integrin alpha E Antibody
Catalog: AF5155
Description: Rabbit polyclonal antibody to Integrin alpha E
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine
Mol.Wt.: 130 kDa; 130kD(Calculated).
Uniprot: P38570
RRID: AB_2837641

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(86%)
Clonality:
Polyclonal
Specificity:
Integrin alpha E Antibody detects endogenous levels of total Integrin alpha E.
RRID:
AB_2837641
Cite Format: Affinity Biosciences Cat# AF5155, RRID:AB_2837641.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A530055J10; alpha-E1; alpha-M290; aM290; Antigen CD103; CD 103; CD103; CD103 antigen; HML 1 antigen; HML-1 antigen; HML1 antigen; Human mucosal lymphocyte antigen 1; Human mucosal lymphocyte antigen 1, alpha subunit; Huminae; Integrin alpha E heavy chain; Integrin alpha E light chain; Integrin alpha E, epithelial-associated; Integrin alpha E1; Integrin alpha IEL; Integrin alpha M290; Integrin alpha-E heavy chain; Integrin alpha-IEL; Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); Integrin, alpha E; ITAE_HUMAN; Itgae; MGC141996; MGC183601; Mucosal lymphocyte 1 antigen; Mucosal lymphocyte antigen 1;

Immunogens

Immunogen:
Uniprot:

P38570(ITAE_HUMAN) >>Visit HPA database.

Gene(ID):
ITGAE(3682)
Expression:
P38570 ITAE_HUMAN:

Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.

Description:
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7.
Sequence:
MWLFHTLLCIASLALLAAFNVDVARPWLTPKGGAPFVLSSLLHQDPSTNQTWLLVTSPRTKRTPGPLHRCSLVQDEILCHPVEHVPIPKGRHRGVTVVRSHHGVLICIQVLVRRPHSLSSELTGTCSLLGPDLRPQAQANFFDLENLLDPDARVDTGDCYSNKEGGGEDDVNTARQRRALEKEEEEDKEEEEDEEEEEAGTEIAIILDGSGSIDPPDFQRAKDFISNMMRNFYEKCFECNFALVQYGGVIQTEFDLRDSQDVMASLARVQNITQVGSVTKTASAMQHVLDSIFTSSHGSRRKASKVMVVLTDGGIFEDPLNLTTVINSPKMQGVERFAIGVGEEFKSARTARELNLIASDPDETHAFKVTNYMALDGLLSKLRYNIISMEGTVGDALHYQLAQIGFSAQILDERQVLLGAVGAFDWSGGALLYDTRSRRGRFLNQTAAAAADAEAAQYSYLGYAVAVLHKTCSLSYIAGAPRYKHHGAVFELQKEGREASFLPVLEGEQMGSYFGSELCPVDIDMDGSTDFLLVAAPFYHVHGEEGRVYVYRLSEQDGSFSLARILSGHPGFTNARFGFAMAAMGDLSQDKLTDVAIGAPLEGFGADDGASFGSVYIYNGHWDGLSASPSQRIRASTVAPGLQYFGMSMAGGFDISGDGLADITVGTLGQAVVFRSRPVVRLKVSMAFTPSALPIGFNGVVNVRLCFEISSVTTASESGLREALLNFTLDVDVGKQRRRLQCSDVRSCLGCLREWSSGSQLCEDLLLMPTEGELCEEDCFSNASVKVSYQLQTPEGQTDHPQPILDRYTEPFAIFQLPYEKACKNKLFCVAELQLATTVSQQELVVGLTKELTLNINLTNSGEDSYMTSMALNYPRNLQLKRMQKPPSPNIQCDDPQPVASVLIMNCRIGHPVLKRSSAHVSVVWQLEENAFPNRTADITVTVTNSNERRSLANETHTLQFRHGFVAVLSKPSIMYVNTGQGLSHHKEFLFHVHGENLFGAEYQLQICVPTKLRGLQVVAVKKLTRTQASTVCTWSQERACAYSSVQHVEEWHSVSCVIASDKENVTVAAEISWDHSEELLKDVTELQILGEISFNKSLYEGLNAENHRTKITVVFLKDEKYHSLPIIIKGSVGGLLVLIVILVILFKCGFFKRKYQQLNLESIRKAQLKSENLLEEEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
86
Bovine
86
Horse
44
Dog
44
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38570 As Substrate

Site PTM Type Enzyme
T350 Phosphorylation
S359 Phosphorylation
T364 Phosphorylation
Y372 Phosphorylation
Y384 Phosphorylation
S388 Phosphorylation
T392 Phosphorylation
Y549 Phosphorylation
Y551 Phosphorylation
S561 Phosphorylation
K885 Acetylation
S1163 Phosphorylation

Research Backgrounds

Function:

Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.

Subunit Structure:

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7.

Family&Domains:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

References

1). Kaempferol alleviates calcium oxalate crystal-induced renal injury and crystal deposition via regulation of the AR/NOX2 signaling pathway. PHYTOMEDICINE, 2021 (PubMed: 33852977) [IF=7.9]
2). A preclinical study: correlation between PD-L1 PET imaging and the prediction of therapy efficacy of MC38 tumor with 68Ga-labeled PD-L1 targeted nanobody. Aging-US, 2021 (PubMed: 33910164) [IF=5.2]

Application: IF/ICC    Species: Human    Sample: MC38 tumors

Figure 7 The expression of CD39, CD103, and CD8 were tested by immunofluorescence of MC38 tumors (n=3, Scale bar=1000 μm). (A) MC38-hPD-L1 control (B) MC38-hPD-L1 (C) MC38-hPD-L1/KO (D) MC38-KO CD8 was indicated by red signals; CD39 or CD103 was indicated by green signals; nuclei, blue 4’,6-diamidino-2-phenylindole (DAPI) signals. Local amplifies the area indicated by the white box. Scale bar=200 μm.
3). Integrin αEβ7 is involved in the intestinal barrier injury of sepsis. Imaging, 2022 (PubMed: 35042191) [IF=0.4]

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