CD34 Antibody - #AF5149

FIGURE 10 BHS chemosensitises breast cancer in immunocompetent Balb/c mice by suppressing EV-Apo/TAM/PD-L1 signalling. (a) Representative images of tumours as well as the statistical analysis of tumour weights, tumour volumes and body weights of Balb/c mice among different groups. Paclitaxel (10 mg/kg/3d) and BHS (20 mg/kg/d) were administrated intraperitoneally, whereas EVs (200 µg/kg/3d) were administrated by a peritumoral injection. n = 9. (b) Representative images of the in vivo imaging assay, the harvested lungs and the lung HE staining assay. n = 3. (c, d) Infiltration levels of CD45+/F4/80+/CD206+ TAMs and CD45+/F4/80+/PD-L1+ TAMs in the TME of mice among different groups. n = 3. (e) Perforin/granzyme B production activity of cytotoxic CD8+ T cells in the TME of mice among different groups. n = 3. (f) Immunofluorescence analysis of TSG101 (EV-specific marker) and CXCL1 expression levels in breast tumour tissues of different groups. (g) Immunofluorescence analysis of CD34 (vascular marker) in breast tumour tissues and lung metastatic foci of different groups. *p < 0.05, **p < 0.01.

Fig. 5. Chondrocyte apoptosis and immune molecular biomarkers were inhibited by BBR binding to SP-D in vivo in OA. OA model rats (n = 5/group) were injected using 25 μl of 200 μM BBR, followed by 25 μl rhSP-D (40 μg/mL) 1 day later. Sham-operated and OA-induction groups received an injection of 50 μl PBS into the right knee joint. For rats receiving rAAV-GFP or rAAV-SP-D shRNA, injections were performed for 10 consecutive days beginning 1 day after the initial BBR injection. At 10 weeks post-surgery, animals were sacrificed and the knee joints were collected for TUNEL and IHC analysis. (A) TUNEL staining (400 ×) was used to assess apoptosis. The p-p65, TLR4, F4/80, CD68, and CD34 expression was assessed via IHC (400 × with higher 800 × magnification inset). (B) Quantification of the indicated proteins in IHC images. Each column represents the mean ± SEM (n = 5). ***P < 0.001 versus the sham-operated group; ##P < 0.01 and ###P < 0.001 versus the OA-induction group; &P < 0.05 and &&P < 0.01 versus the OA + BBR group.

(D) Representative microscopic feature of subtaneous tumor in H&E-stained sections of nude mice (left). CD34 protein expression was higher in tumors with low expression of BCL2L10 by IHC (middle and right).