ZO 1 Antibody | Affinity Biosciences

Product:	ZO 1 Antibody
Catalog:	AF5145
Description:	Rabbit polyclonal antibody to ZO 1
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat, Pig, Monkey
Prediction:	Horse, Dog
Mol.Wt.:	195 kDa; 195kD(Calculated).
Uniprot:	Q07157
RRID:	AB_2837631

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Pig,Monkey

Prediction:

Horse(85%), Dog(92%)

Clonality:

Polyclonal

Specificity:

ZO 1 Antibody detects endogenous levels of total ZO 1.

RRID:

AB_2837631
Cite Format: Affinity Biosciences Cat# AF5145, RRID:AB_2837631.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

Immunogens

Immunogen:

A synthesized peptide derived from human ZO 1, corresponding to a region within the internal amino acids.

Uniprot:

Q07157(ZO1_HUMAN) >>Visit HPA database.

Gene(ID):

TJP1(7082)

Expression:

Q07157 ZO1_HUMAN:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Description:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Sequence:

Q07157 ZO1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Horse

Chicken

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q07157_ZO1_HUMAN

Function:

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).

PTMs:

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Cell junction. Cell junction>Gap junction. Cell projection>Podosome.
Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113).

Tissue Specificity:

Family&Domains:

The 244-aa domain between residues 633 and 876 is the primary occludin (OCLN)-binding site and is required for stable association with the tight junction (PubMed:9792688).

The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of TJP1 at junctions (PubMed:9792688, PubMed:12354695, PubMed:20930113). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (PubMed:12354695). The ABR is also necessary for TJP1 recruitment to podosomes (PubMed:20930113).

The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with TJP2 and TJP3 (PubMed:9792688, PubMed:17928286).

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

References

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]

2). Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria. Nature biomedical engineering, 2024 (PubMed: 38839928) [IF=26.8]

3). Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality. Nature biomedical engineering, 2025 (PubMed: 40000896) [IF=26.8]

4). Dietary fiber alleviates alcoholic liver injury via Bacteroides acidifaciens and subsequent ammonia detoxification. Cell host & microbe, 2024 (PubMed: 38959900) [IF=20.6]

5). Targeting P2Y14R protects against necroptosis of intestinal epithelial cells through PKA/CREB/RIPK1 axis in ulcerative colitis. Nature communications, 2024 (PubMed: 38453952) [IF=16.6]

6). Chemical reaction-mediated covalent localization of bacteria. Nature Communications, 2022 (PubMed: 36528693) [IF=16.6]

7). Restoring mucosal barrier homeostasis by in situ formation of a living-synthetic therapeutic coating. Nature communications, 2025 (PubMed: 40825983) [IF=16.6]

Application: IF/ICC Species: Mouse Sample:

Fig. 4: Restoration of the mucosal physicochemical barrier. A Images of AB staining of LPS-stimulated HT29-MTX-E12 cells incubated with PBS or 0.2 mg/ml Bi(NO3)3·5H2O for 12 h. B TEER data of LPS-stimulated epithelial cell monolayer in transwell insert after incubation with PBS or 3 μg/ml Bi(NO3)3·5H2O for 24 h (n = 3 independent samples). C Concentrations of FITC-dextran in the basolateral medium of transwell measured by a microplate fluorometer after incubation with PBS or 3 μg/ml Bi(NO3)3·5H2O for 4 or 12 h (n = 3 independent samples). Control group indicates unstimulated cells in (A–C). D-H Evaluation of vaginal barrier integrity of S. aureus-infected mice after intravaginal treatment with PBS, LGG, EG-Bi, or EG-Bi-LGG every day for 7 days. D Images of AF488-WGA (red) staining of vaginal sections from treated mice. Blue indicates cell nuclei stained with 4’,6-diamidino-2-phenylindole (DAPI). E Average fluorescence intensity of AF488-WGA of vaginal sections from D (n = 4 independent samples). F Immunofluorescence images of vaginal occludin (green) and ZO-1 (red) expressions from treated mice. Blue indicates cell nuclei stained with DAPI. Average fluorescence intensities of occludin (G) and ZO-1 (H) of vaginal sections from (F) (n = 3 independent samples). Data are mean ± SD. Statistical analysis was performed using one-way ANOVA with Fisher’s LSD multiple comparisons test, and p-values were indicated. Source data are provided as a Source Data file.

8). Novel Role of Gut-Derived Roseburia Intestinalis in Safeguarding Intestinal Barrier Integrity and Microenvironment Homeostasis During Arsenic Exposure. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2025 (PubMed: 40831218) [IF=15.1]

Application: WB Species: Mouse Sample:

Figure 1 Arsenic disrupts the mechanical barrier of intestine in the mice. A) Representative hematoxylin and eosin (H&E) stained images of intestine tissues. Scale bars, 100 µm (top) and 50 µm (bottom). B–D) Total pathological score, smooth muscle thickness, and mucosa depth of the intestine tissues (n = 3). E) Representative Alcian Blue-Periodic Acid-Schiff (AB-PAS) stained images of intestine tissues. Scale bars, 100 µm (top) and 50 µm (bottom). F) The number of goblet cells of the intestine tissues (n = 3). G,H) The mRNA expressions of Tjp-1 and occludin in intestines of mice (n = 3). I–K) Representative western blot images and statistical analyses of ZO-1 and Occludin in intestines of mice (n = 2).

9). The role of the microbiota–gut–brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism. Acta Pharmaceutica Sinica B, 2024 (PubMed: 39664442) [IF=14.7]

10). Phytosphingosine contributes gut microbiota metabolism to alleviate low-grade endotoxemia-induced mastitis.. Microbiome, 2025 [IF=13.5]

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ZO 1 Antibody - #AF5145