CD57 Antibody - #AF5125

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Product: CD57 Antibody
Catalog: AF5125
Description: Rabbit polyclonal antibody to CD57
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 38 kDa; 38kD(Calculated).
Uniprot: Q9P2W7
RRID: AB_2837611

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(82%), Dog(80%)
Clonality:
Polyclonal
Specificity:
CD57 Antibody detects endogenous levels of total CD57.
RRID:
AB_2837611
Cite Format: Affinity Biosciences Cat# AF5125, RRID:AB_2837611.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3-glucuronyltransferase 1; 3-glucuronyltransferase; B3GA1_HUMAN; B3gat1; Beta 1 3 glucuronyltransferase 1; Beta-1; Beta-1,3-glucuronyltransferase 1; CD 57; CD57; CD57 antigen; Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT P; GlcAT-P; GLCATP; GlcUAT P; GlcUAT-P; GlcUATP; Glucuronosyltransferase P; HNK 1; HNK1; LEU 7; LEU7; LEU7 antigen; NK 1; NK1; UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase; UDP-GlcUA:glycoprotein beta-1;

Immunogens

Immunogen:
Uniprot:

Q9P2W7(B3GA1_HUMAN) >>Visit HPA database.

Gene(ID):
B3GAT1(27087)
Expression:
Q9P2W7 B3GA1_HUMAN:

Mainly expressed in the brain.

Description:
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin.
Sequence:
MPKRRDILAIVLIVLPWTLLITVWHQSTLAPLLAVHKDEGSDPRRETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAELTRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARDPRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWPVAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLRGVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
82
Dog
80
Bovine
70
Sheep
70
Chicken
64
Rabbit
64
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9P2W7 As Substrate

Site PTM Type Enzyme
Y282 Phosphorylation

Research Backgrounds

Function:

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

PTMs:

The soluble form derives from the membrane form by proteolytic processing.

Subcellular Location:

Golgi apparatus membrane>Single-pass type II membrane protein. Secreted.

Golgi apparatus membrane>Single-pass type II membrane protein. Endoplasmic reticulum membrane. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in the brain.

Subunit Structure:

Homodimer. Interacts with SAR1A.

Family&Domains:

Belongs to the glycosyltransferase 43 family.

Research Fields

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Frontiers in Immunology, 2022 (PubMed: 35350787) [IF=7.3]

Application: IHC    Species: Mice    Sample: bronchoalveolar lavage fluid (BRLF) and lung tissues

Figure 8 Immune cell infiltration in COPD mouse models. (A) Body weight of mice. (B) The mean alveolar septal thickness (MAST). (C) Mean linear intercept (MLI). (D) Destructive index (DI). (E) Representative HE staining images (100x and 400x). (F) The proportion of immune cells. (G) The expression of IL-6 in bronchoalveolar lavage fluid (BRLF) and lung tissues of mice. (H) The expression of IL-1β in BRLF and lung tissues of mice. (I) The expression of TNF-α in BRLF and lung tissues of mice. (J) Representative immunohistochemistry images of CD8a and CD57 (400x). (K) Representative immunofluorescence images of CD19 and CD20 (400x). For panel G, H, and I, the unit for BRLF is pg/mL, and the unit for lung tissue is pg/mg. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Control.

Application: IHC    Species: mouse    Sample: lung

FIGURE 8 | Immune cell infiltration in COPD mouse models.(J) Representative immunohistochemistry images of CD8a and CD57 (400x).

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