Connexin 26 Antibody | Affinity Biosciences

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Connexin 26 Antibody - Western blot analysis of Connexin 26 expression in Jurkat cells,The lane on the left was treated with the antigen-specific peptide.

Product:	Connexin 26 Antibody
Catalog:	AF5116
Description:	Rabbit polyclonal antibody to Connexin 26
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	26 kDa; 26kD(Calculated).
Uniprot:	P29033
RRID:	AB_2837602

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Connexin 26 Antibody detects endogenous levels of total Connexin 26.

RRID:

AB_2837602
Cite Format: Affinity Biosciences Cat# AF5116, RRID:AB_2837602.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

connexin 26; Connexin-26; Cx26; CXB2_HUMAN; DFNA3; DFNB1; Gap junction beta-2 protein; GJB2; HID; KID; NSRD1; PPK;

Immunogens

Immunogen:

Uniprot:

P29033(CXB2_HUMAN) >>Visit HPA database.

Gene(ID):

GJB2(2706)

Description:

A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence:

P29033 CXB2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQADFVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRHEKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAFMYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSGICILLNVTELCYLLIRYCSGKSKKPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29033 As Substrate

P29033

Site	PTM Type	Source
K15	Acetylation	Uniprot
R75	Methylation	Uniprot
K102	Acetylation	Uniprot
K103	Acetylation	Uniprot
K105	Acetylation	Uniprot
K108	Acetylation	Uniprot
K108	Ubiquitination	Uniprot
K112	Acetylation	Uniprot
K112	Ubiquitination	Uniprot
K116	Acetylation	Uniprot
K116	Ubiquitination	Uniprot
K122	Sumoylation	Uniprot
K122	Ubiquitination	Uniprot
T123	Phosphorylation	Uniprot
T177	Phosphorylation	Uniprot
S183	Phosphorylation	Uniprot
T186	Phosphorylation	Uniprot
K221	Methylation	Uniprot
K223	Methylation	Uniprot

Research Backgrounds

P29033_CXB2_HUMAN

Function:

Structural component of gap junctions. Gap junctions are dodecameric channels that connect the cytoplasm of adjoining cells. They are formed by the docking of two hexameric hemichannels, one from each cell membrane. Small molecules and ions diffuse from one cell to a neighboring cell via the central pore.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.
Note: Colocalizes with GJB4 at gap junction plaques in the cochlea.

Subunit Structure:

A hemichannel or connexon is composed of a hexamer of connexins. A functional gap junction is formed by the apposition of two hemichannels. Interacts with CNST. Forms heteromeric channels with GJB4 (By similarity).

Family&Domains:

Belongs to the connexin family. Beta-type (group I) subfamily.

References

1). Disruption of Epithelial Barrier of Caco-2 Cell Monolayers by excretory secretory products of Trichinella Spiralis might be Related to serine Protease. Frontiers in Microbiology, 2021 (PubMed: 33815318) [IF=5.2]

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Connexin 26 Antibody - #AF5116