Connexin 45 / GJA7 Antibody - #AF5108

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Product: Connexin 45 / GJA7 Antibody
Catalog: AF5108
Description: Rabbit polyclonal antibody to Connexin 45 / GJA7
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 45 kDa; 45kD(Calculated).
Uniprot: P36383
RRID: AB_2837594

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(89%), Horse(89%), Rabbit(89%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Connexin 45 / GJA7 Antibody detects endogenous levels of total Connexin 45 / GJA7.
RRID:
AB_2837594
Cite Format: Affinity Biosciences Cat# AF5108, RRID:AB_2837594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Connexin 45; Connexin-45; Connexin45; CX 31.9; CX 45; CX31.9; Cx45; CXG1_HUMAN; DKFZp686P0738; Gap junction alpha 7 protein; Gap junction alpha-7 protein; Gap junction gamma 1 protein; Gap junction gamma-1 protein; Gap junction protein alpha 7 45kDa; Gap junction protein alpha 7; Gap junction protein, gamma 1, 45kDa; GJA 7; GJA7; GJC1; GJD3;

Immunogens

Immunogen:
Uniprot:

P36383(CXG1_HUMAN) >>Visit HPA database.

Gene(ID):
GJC1(10052)
Description:
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Sequence:
MSWSFLTRLLEEIHNHSTFVGKIWLTVLIVFRIVLTAVGGESIYYDEQSKFVCNTEQPGCENVCYDAFAPLSHVRFWVFQIILVATPSVMYLGYAIHKIAKMEHGEADKKAARSKPYAMRWKQHRALEETEEDNEEDPMMYPEMELESDKENKEQSQPKPKHDGRRRIREDGLMKIYVLQLLARTVFEVGFLIGQYFLYGFQVHPFYVCSRLPCPHKIDCFISRPTEKTIFLLIMYGVTGLCLLLNIWEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKTSVWI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Horse
89
Bovine
89
Rabbit
89
Sheep
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36383 As Substrate

Site PTM Type Enzyme
K115 Methylation
Y141 Phosphorylation
S148 Phosphorylation
K150 Ubiquitination
K295 Ubiquitination
Y301 Phosphorylation
K308 Ubiquitination
K312 Ubiquitination
K315 Ubiquitination
Y324 Phosphorylation
Y356 Phosphorylation
S357 Phosphorylation
K371 Acetylation
K380 Ubiquitination
K391 Ubiquitination

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

A connexon is composed of a hexamer of connexins. Interacts with CNST (By similarity).

Family&Domains:

Belongs to the connexin family. Gamma-type subfamily.

References

1). Cinnamic acid preserves against myocardial ischemia/reperfusion injury via suppression of NLRP3/Caspase-1/GSDMD signaling pathway. Phytomedicine, 2022 (PubMed: 35320770) [IF=6.7]
2). Insulin gene enhancer binding protein 1 induces adipose tissue‑derived stem cells to differentiate into pacemaker‑like cells. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2019 (PubMed: 30483766) [IF=5.7]

Application: WB    Species: rat    Sample: adipose tissue‑derived stem cells

Figure 3.| Expression of related genes by western blot and RT‑qPCR analyses following co‑culture for 5‑7 days. (A) Gene expression levels of HCN4, Cx45 and Cx43 were examined by RT‑qPCR analysis. (B) Protein expression of HCN4, Cx45 and Cx43 was examined using western blotting.
3). Cardioprotective effect of cinnamaldehyde pretreatment on ischemia/ reperfusion injury via inhibiting NLRP3 inflammasome activation and gasdermin D mediated cardiomyocyte pyroptosis. Chemico-Biological Interactions, 2022 (PubMed: 36341777) [IF=4.7]
4). A new combination of transcription factors increases the harvesting efficiency of pacemaker‑like cells. Molecular Medicine Reports, 2019 (PubMed: 30864738) [IF=3.4]

Application: WB    Species: rat    Sample: ADSCs

Figure 2. |Expression of associated genes by RT‑qPCR and western blotting after co‑culture for 7 days. (A) HCN4, cTnT, Tbx3, Nkx2.5, Cx43 and Cx45 gene expression was examined using RT‑qPCR. (B) HCN4, Cx43 and Cx45 protein expression examined using western blotting.
5). TGF-β1 and connexin-43 expression in neurogenic bladder from rats with sacral spinal cord injury. Neurourology and Urodynamics, 2018 (PubMed: 30070388) [IF=1.8]

Application: WB    Species: rat    Sample: sacral spinal cord

FIGURE 2 |Changes in CX43, pCX43, TGF-β1, Smad3, pSmad3, and CX45 levels examined by Western blot in detrusor after sacral spinal injury. The density of proteins was normalized with the density of the bands of β-actin. CX43 (B) and pCX43 (C) showed a significant decrease but TGF-β1 (D), Smad3 (E), and pSmad3 (F) showed a significant increase in the bladder detrusor after SCI. Those changes were more significant in transection than in hemisection of sacral spine cord. CX45 was not changed among three groups.

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