Cytokeratin 13 Antibody - #AF5102

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Product: Cytokeratin 13 Antibody
Catalog: AF5102
Description: Rabbit polyclonal antibody to Cytokeratin 13
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 50 kDa; 50kD(Calculated).
Uniprot: P13646
RRID: AB_2837588

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 13 Antibody detects endogenous levels of total Cytokeratin 13.
RRID:
AB_2837588
Cite Format: Affinity Biosciences Cat# AF5102, RRID:AB_2837588.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

47 kDa cytokeratin; CK-13; CK13; Cytokeratin 13; Cytokeratin-13; K13; K1C13_HUMAN; Ka13; Keratin 13; Keratin; keratin type I cytoskeletal 13; Keratin-13; Krt-1.13; Krt1-13; KRT13; MGC161462; MGC3781; type I cytoskeletal 13; Type I keratin Ka13; WSN2;

Immunogens

Immunogen:
Uniprot:

P13646(K1C13_HUMAN) >>Visit HPA database.

Gene(ID):
KRT13(3860)
Expression:
P13646 K1C13_HUMAN:

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

Description:
Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains.
Sequence:
MSLRLQSSSASYGGGFGGGSCQLGGGRGVSTCSTRFVSGGSAGGYGGGVSCGFGGGAGSGFGGGYGGGLGGGYGGGLGGGFGGGFAGGFVDFGACDGGLLTGNEKITMQNLNDRLASYLEKVRALEEANADLEVKIRDWHLKQSPASPERDYSPYYKTIEELRDKILTATIENNRVILEIDNARLAADDFRLKYENELALRQSVEADINGLRRVLDELTLSKTDLEMQIESLNEELAYMKKNHEEEMKEFSNQVVGQVNVEMDATPGIDLTRVLAEMREQYEAMAERNRRDAEEWFHTKSAELNKEVSTNTAMIQTSKTEITELRRTLQGLEIELQSQLSMKAGLENTVAETECRYALQLQQIQGLISSIEAQLSELRSEMECQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMIGFPSSAGSVSPRSTSVTTTSSASVTTTSNASGRRTSDVRRP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
91
Horse
82
Pig
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13646 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
Y12 Phosphorylation
R27 Methylation
R35 Methylation
Y118 Phosphorylation
S144 Phosphorylation
S147 Phosphorylation
Y152 Phosphorylation
S153 Phosphorylation
Y155 Phosphorylation
Y156 Phosphorylation
T168 Phosphorylation
T170 Phosphorylation
Y194 Phosphorylation
Y238 Phosphorylation
Y281 Phosphorylation
T311 Phosphorylation
T316 Phosphorylation
T327 Phosphorylation
S337 Phosphorylation
S340 Phosphorylation
T404 Phosphorylation
Y405 Phosphorylation
S421 Phosphorylation
S422 Phosphorylation
S425 Phosphorylation
S427 Phosphorylation
S432 Phosphorylation
T434 Phosphorylation
T452 Phosphorylation
S453 Phosphorylation

Research Backgrounds

PTMs:

O-glycosylated; glycans consist of single N-acetylglucosamine residues.

Tissue Specificity:

Expressed in some epidermal sweat gland ducts (at protein level) and in exocervix, esophagus and placenta.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. keratin-13 is generally associated with keratin-4.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Cytokeratin 13 promotes radiotherapy sensitivity of nasopharyngeal carcinoma by downregulating the MEK/ERK pathway. IUBMB LIFE (PubMed: 35426961) [IF=4.6]

Application: WB    Species: Human    Sample: NPC cells

FIGURE 1CK13 promoted the sensitivity of NPC HNE-3 cells to radiotherapy. (a, b) qRT-PCR and Western blotting detected the mRNA expression and protein levels of CK13 in a variety of NPC cells, respectively. Asterisks represent statistically significant differences (*p 

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