TSC1 Antibody - #AF5101

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Product: TSC1 Antibody
Catalog: AF5101
Description: Rabbit polyclonal antibody to TSC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Sheep, Dog
Mol.Wt.: 150 kDa; 130kD(Calculated).
Uniprot: Q92574
RRID: AB_2837587

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(92%), Sheep(83%), Dog(92%)
Clonality:
Polyclonal
Specificity:
TSC1 Antibody detects endogenous levels of total TSC1.
RRID:
AB_2837587
Cite Format: Affinity Biosciences Cat# AF5101, RRID:AB_2837587.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hamartin; kiaa0243; LAM; TSC; Tsc1; Tsc1 gene; TSC1_HUMAN; Tuberous sclerosis 1; Tuberous sclerosis 1 protein; tumor suppressor;

Immunogens

Immunogen:
Uniprot:

Q92574(TSC1_HUMAN) >>Visit HPA database.

Gene(ID):
TSC1(7248)
Expression:
Q92574 TSC1_HUMAN:

Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.

Description:
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
Sequence:
MAQQANVGELLAMLDSPMLGVRDDVTAVFKENLNSDRGPMLVNTLVDYYLETSSQPALHILTTLQEPHDKHLLDRINEYVGKAATRLSILSLLGHVIRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITMLPMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVAEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPTEASYEDGYSVSHQISARFPHRSADVTTSPYADTQNSYGCATSTPYSTSRLMLLNMPGQLPQTLSSPSTRLITEPPQATLWSPSMVCGMTTPPTSPGNVPPDLSHPYSKVFGTTAGGKGTPLGTPATSPPPAPLCHSDDYVHISLPQATVTPPRKEERMDSARPCLHRQHHLLNDRGSEEPPGSKGSVTLSDLPGFLGDLASEEDSIEKDKEEAAISRELSEITTAEAEPVVPRGGFDSPFYRDSLPGSQRKTHSAASSSQGASVNPEPLHSSLDKLGPDTPKQAFTPIDLPCGSADESPAGDRECQTSLETSIFTPSPCKIPPPTRVGFGSGQPPPYDHLFEVALPKTAHHFVIRKTEELLKKAKGNTEEDGVPSTSPMEVLDRLIQQGADAHSKELNKLPLPSKSVDWTHFGGSPPSDEIRTLRDQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYNQLQEQRDTMVTKLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIAELRIELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQNKHSDTTKEVEMMKAAYRKELEKNRSHVLQQTQRLDTSQKRILELESHLAKKDHLLLEQKKYLEDVKLQARGQLQAAESRYEAQKRITQVFELEILDLYGRLEKDGLLKKLEEEKAEAAEAAEERLDCCNDGCSDSMVGHNEEASGHNGETKTPRPSSARGSSGSRGGGGSSSSSSELSTPEKPPHQRAGPFSSRWETTMGEASASIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDGMTSSLSESLKTELGKDLGVEAKIPLNLDGPHPSPPTPDSVGQLHIMDYNETHHEHS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Dog
92
Sheep
83
Xenopus
40
Pig
0
Bovine
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92574 As Substrate

Site PTM Type Enzyme
Y79 Phosphorylation
S91 Phosphorylation
S102 Phosphorylation
S108 Phosphorylation
S115 Phosphorylation
S205 Phosphorylation
K221 Ubiquitination
K238 Ubiquitination
T267 Phosphorylation
S270 Phosphorylation
Y271 Phosphorylation
Y275 Phosphorylation
S282 Phosphorylation
S295 Phosphorylation
Y297 Phosphorylation
S303 Phosphorylation
Y304 Phosphorylation
T310 Phosphorylation
Y312 Phosphorylation
T357 Phosphorylation
T360 Phosphorylation
T390 Phosphorylation
T393 Phosphorylation
S394 Phosphorylation
Y406 Phosphorylation
T417 Phosphorylation P06493 (CDK1)
S427 Phosphorylation
S468 Phosphorylation
S472 Phosphorylation
K477 Ubiquitination
S483 Phosphorylation
S487 Phosphorylation O14920 (IKBKB)
T490 Phosphorylation
S505 Phosphorylation
Y508 Phosphorylation
S511 Phosphorylation O14920 (IKBKB)
S515 Phosphorylation
S521 Phosphorylation
T547 Phosphorylation
S579 Phosphorylation
S584 Phosphorylation P06493 (CDK1)
S598 Phosphorylation
Y604 Phosphorylation
K662 Ubiquitination
K672 Ubiquitination
S673 Phosphorylation
S682 Phosphorylation
K725 Ubiquitination
K737 Ubiquitination
K751 Acetylation
K756 Ubiquitination
K774 Ubiquitination
S871 Phosphorylation
T873 Phosphorylation
T874 Phosphorylation
K934 Ubiquitination
T1020 Phosphorylation
S1024 Phosphorylation
S1025 Phosphorylation
S1029 Phosphorylation
S1030 Phosphorylation
R1033 Methylation
S1046 Phosphorylation
T1047 Phosphorylation P06493 (CDK1)
S1080 Phosphorylation
S1100 Phosphorylation
S1102 Phosphorylation
S1114 Phosphorylation
S1116 Phosphorylation
S1141 Phosphorylation
T1144 Phosphorylation

Research Backgrounds

Function:

In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1. Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity. Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1.

PTMs:

Phosphorylation at Ser-505 does not affect interaction with TSC2.

Subcellular Location:

Cytoplasm. Membrane>Peripheral membrane protein.
Note: At steady state found in association with membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in skeletal muscle, followed by heart, brain, placenta, pancreas, lung, liver and kidney. Also expressed in embryonic kidney cells.

Subunit Structure:

Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. Forms a complex composed of chaperones HSP90 and HSP70, co-chaperones CDC37, PPP5C, TSC1 and client protein TSC2, CDK4, AKT, RAF1 and NR3C1; this complex does not contain co-chaperones STIP1/HOP and PTGES3/p23. Forms a complex containing HSP90AA1, TSC1 and TSC2; TSC1 is required to recruit TCS2 to the complex. Interacts (via C-terminus) with the closed form of HSP90AA1 (via the middle domain and TPR repeat-binding motif). Interacts with TSC2; the interaction stabilizes TSC2 and prevents TSC2 self-aggregation. Interacts with DOCK7. Interacts with FBXW5. Interacts with TBC1D7. Interacts with WDR45B. Interacts with RPAP3 and URI1.

Family&Domains:

The C-terminal putative coiled-coil domain is necessary for interaction with TSC2.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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