Product: His-Tag Rabbit pAb
Catalog: T0051
Source: Rabbit
Application: WB, ELISA(peptide)
Reactivity: All
Uniprot:
RRID: AB_2837584
   Size Price Inventory
 50ul $150 In stock
 100ul $250 In stock
 200ul $350 In stock
 1ml $1200 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:3000-1:10000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Reactivity:
All
Clonality:
Polyclonal
Specificity:
The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
RRID:
AB_2837584
Cite Format: Affinity Biosciences Cat# T0051, RRID:AB_2837584.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Immunogens

Immunogen:

Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

Description:
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

References

1). Liu X et al. The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging (Albany NY) 2020 Dec 3;12. (PubMed: 33290257) [IF=5.955]

2). Rao XJ et al. Characterization of a dual-CRD galectin in the silkworm Bombyx mori. Dev Comp Immunol 2016 Jul;60:149-59 (PubMed: 26944801) [IF=3.605]

Application: WB    Species:    Sample:


3). Zhan MY et al. A single-CRD C-type lectin is important for bacterial clearance in the silkworm. Dev Comp Immunol 2016 Dec;65:330-339 (PubMed: 27519466) [IF=3.605]

4). Cao X et al. Expression and characterization of recombinant humanized anti-HER2 single-chain antibody in Pichia pastoris for targeted cancer therapy. Biotechnol Lett 2015 Jul;37(7):1347-54 (PubMed: 25735855)

Application: IF/ICC    Species:    Sample:


5). Shahzad T et al. Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori. Arch Insect Biochem Physiol 2017 Jul;95(3) (PubMed: 28618068)

6). A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.