Product: His-Tag Rabbit pAb
Catalog: T0051
Description: Rabbit polyclonal antibody to His-Tag pAb
Application: WB
Reactivity: All
RRID: AB_2837584

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Product Info

WB 1:3000-1:10000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Cite Format: Affinity Biosciences Cat# T0051, RRID:AB_2837584.
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and glycerol. Store at -20 °C. Stable for 12 months from date of receipt.



Rabbit polyclonal antibody is prepared by immunizing synthetic peptide coupled to KLH.

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.


1). Lipopolysaccharide-binding protein expression is increased by stress and inhibits monoamine synthesis to promote depressive symptoms. Immunity, 2023 (PubMed: 36854305) [IF=32.4]

2). A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity. CANCER LETTERS, 2020 (PubMed: 32805322) [IF=9.7]

3). The 1316T> C missenses mutation in MTHFR contributes to MTHFR deficiency by targeting MTHFR to proteasome degradation. Aging-US, 2021 (PubMed: 33290257) [IF=5.2]

4). Characterization of a dual-CRD galectin in the silkworm Bombyx mori. Developmental & Comparative Immunology, 2016 (PubMed: 26944801) [IF=2.9]

Application: WB    Species:    Sample:

5). A single-CRD C-type lectin is important for bacterial clearance in the silkworm. Developmental & Comparative Immunology, 2016 (PubMed: 27519466) [IF=2.9]

6). Expression and characterization of recombinant humanized anti-HER2 single-chain antibody in Pichia pastoris for targeted cancer therapy. BIOTECHNOLOGY LETTERS, 2015 (PubMed: 25735855) [IF=2.7]

Application: IF/ICC    Species:    Sample:

7). Molecular cloning and analysis of a C-type lectin from silkworm Bombyx mori. Archives of Insect Biochemistry and Physiology, 2017 (PubMed: 28618068) [IF=2.2]

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