PAR4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HeLa cells using PAR4 antibody.

Product:	PAR4 Antibody
Catalog:	DF5212
Description:	Rabbit polyclonal antibody to PAR4
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep
Mol.Wt.:	41 KD; 41kD(Calculated).
Uniprot:	Q96RI0
RRID:	AB_2837561

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Bovine(89%), Sheep(89%)

Clonality:

Polyclonal

Specificity:

PAR4 Antibody detects endogenous levels of total PAR4.

RRID:

AB_2837561
Cite Format: Affinity Biosciences Cat# DF5212, RRID:AB_2837561.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Coagulation factor II (thrombin) receptor like 3; Coagulation factor II receptor like 3; Coagulation factor II receptor-like 3; F2RL3; PAR 4; PAR-4; PAR4; PAR4_HUMAN; protease activated receptor 4; protease activated receptor4; Proteinase activated receptor 4; Proteinase-activated receptor 4; Thrombin receptor like 3; Thrombin receptor-like 3;

Immunogens

Immunogen:

Uniprot:

Q96RI0(PAR4_HUMAN) >>Visit HPA database.

Gene(ID):

F2RL3(9002)

Expression:

Q96RI0 PAR4_HUMAN:

Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.

Sequence:

Q96RI0 PAR4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWGRLLLWPLVLGFSLSGGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRALLLGWVPTRLVPALYGLVLVVGLPANGLALWVLATQAPRLPSTMLLMNLAAADLLLALALPPRIAYHLRGQRWPFGEAACRLATAALYGHMYGSVLLLAAVSLDRYLALVHPLRARALRGRRLALGLCMAAWLMAAALALPLTLQRQTFRLARSDRVLCHDALPLDAQASHWQPAFTCLALLGCFLPLLAMLLCYGATLHTLAASGRRYGHALRLTAVVLASAVAFFVPSNLLLLLHYSDPSPSAWGNLYGAYVPSLALSTLNSCVDPFIYYYVSAEFRDKVRAGLFQRSPGDTVASKASAEGGSRGMGTHSSLLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Pig

Dog

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96RI0 As Substrate

Q96RI0

Site	PTM Type	Source
S366	Phosphorylation	Uniprot
K367	Ubiquitination	Uniprot
S369	Phosphorylation	Uniprot
S374	Phosphorylation	Uniprot
S382	Phosphorylation	Uniprot

Research Backgrounds

Q96RI0_PAR4_HUMAN

Function:

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

PTMs:

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Widely expressed, with highest levels in lung, pancreas, thyroid, testis and small intestine. Not expressed in brain, kidney, spinal cord and peripheral blood leukocytes. Also detected in platelets.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Immune system > Platelet activation. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PAR4 Antibody - #DF5212