VIP Receptor 1 Antibody | Affinity Biosciences

IF/ICC
Cites

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Figure 7 Validation of the driver proteins' expression level in each group.

Product:	VIP Receptor 1 Antibody
Catalog:	DF5172
Description:	Rabbit polyclonal antibody to VIP Receptor 1
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.:	52 KD; 52kD(Calculated).
Uniprot:	P32241
RRID:	AB_2837521

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(89%), Rabbit(89%), Chicken(89%)

Clonality:

Polyclonal

Specificity:

VIP Receptor 1 Antibody detects endogenous levels of total VIP Receptor 1.

RRID:

AB_2837521
Cite Format: Affinity Biosciences Cat# DF5172, RRID:AB_2837521.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

FLJ41949; HVR 1; HVR1; PACAP R 2; PACAP type II receptor; PACAP-R-2; PACAP-R2; Pituitary adenylate cyclase activating polypeptide receptor type II; Pituitary adenylate cyclase activating polypeptide type II receptor; Pituitary adenylate cyclase-activating polypeptide type II receptor; RCD1; RDC 1; RDC1; V1RG; VAPC 1; VAPC1; Vasoactive intestinal peptide receptor 1; Vasoactive intestinal polypeptide receptor 1; Vasoactive intestinal polypeptide receptor 1 precursor; VIP and PACAP receptor 1; VIP R 1; VIP receptor type I; VIP-R-1; VIPR 1; VIPR; VIPR1; VIPR1_HUMAN; VIRG; VPAC 1; VPAC1; VPCAP1R;

Immunogens

Immunogen:

Uniprot:

P32241(VIPR1_HUMAN) >>Visit HPA database.

Gene(ID):

VIPR1(7433)

Expression:

P32241 VIPR1_HUMAN:

In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T-cell lines Peer, MOLT-4, HSB and YT.

Sequence:

P32241 VIPR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRPPSPLPARWLCVLAGALAWALGPAGGQAARLQEECDYVQMIEVQHKQCLEEAQLENETIGCSKMWDNLTCWPATPRGQVVVLACPLIFKLFSSIQGRNVSRSCTDEGWTHLEPGPYPIACGLDDKAASLDEQQTMFYGSVKTGYTIGYGLSLATLLVATAILSLFRKLHCTRNYIHMHLFISFILRAAAVFIKDLALFDSGESDQCSEGSVGCKAAMVFFQYCVMANFFWLLVEGLYLYTLLAVSFFSERKYFWGYILIGWGVPSTFTMVWTIARIHFEDYGCWDTINSSLWWIIKGPILTSILVNFILFICIIRILLQKLRPPDIRKSDSSPYSRLARSTLLLIPLFGVHYIMFAFFPDNFKPEVKMVFELVVGSFQGFVVAILYCFLNGEVQAELRRKWRRWHLQGVLGWNPKYRHPSGGSNGATCSTQVSMLTRVSPGARRSSSFQAEVSLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Chicken

Rabbit

Dog

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P32241 As Substrate

P32241

Site	PTM Type	Source
S165	Phosphorylation	Uniprot
S250	Phosphorylation	Uniprot
T429	Phosphorylation	Uniprot
S435	Phosphorylation	Uniprot
S447	Phosphorylation	Uniprot
S448	Phosphorylation	Uniprot
S449	Phosphorylation	Uniprot
S455	Phosphorylation	Uniprot

Research Backgrounds

P32241_VIPR1_HUMAN

Function:

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

References

1). Modular Screening Reveals Driver Induced Additive Mechanisms of Baicalin and Jasminoidin on Cerebral Ischemia Therapy. Frontiers in Cardiovascular Medicine (PubMed: 35265682) [IF=3.6]

Application: WB Species: Rat Sample: hippocampus tissue

Figure 7 Validation of the driver proteins' expression level in each group. (A) The band diagrams of 7 representative driver proteins and β-actin in each group. (B–H) The expression level comparison among groups for PAQR8, RhoA, EMC10, GGA2, VIPR1, and FAM120A, SEMA3F determined by western blot. *p < 0.05, **p < 0.01, ***p < 0.001, and ns, non-significant.

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VIP Receptor 1 Antibody - #DF5172