TAS2R16 Antibody | Affinity Biosciences

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Western blot analysis of extracts from MCF-7 cells, using TAS2R16 antibody.

Product:	TAS2R16 Antibody
Catalog:	DF5160
Description:	Rabbit polyclonal antibody to TAS2R16
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	34 KD; 34kD(Calculated).
Uniprot:	Q9NYV7
RRID:	AB_2837509

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

TAS2R16 Antibody detects endogenous levels of total TAS2R16.

RRID:

AB_2837509
Cite Format: Affinity Biosciences Cat# DF5160, RRID:AB_2837509.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

T2R16; Taste receptor type 2 member 16;

Immunogens

Immunogen:

Uniprot:

Q9NYV7(T2R16_HUMAN) >>Visit HPA database.

Gene(ID):

TAS2R16(50833)

Expression:

Q9NYV7 T2R16_HUMAN:

Expressed in a subset of gustducin-positive taste receptor cells of the tongue. Expressed in circumvallate papillae and testis (PubMed:16720576).

Sequence:

Q9NYV7 T2R16_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIPIQLTVFFMIIYVLESLTIIVQSSLIVAVLGREWLQVRRLMPVDMILISLGISRFCLQWASMLNNFCSYFNLNYVLCNLTITWEFFNILTFWLNSLLTVFYCIKVSSFTHHIFLWLRWRILRLFPWILLGSLMITCVTIIPSAIGNYIQIQLLTMEHLPRNSTVTDKLENFHQYQFQAHTVALVIPFILFLASTIFLMASLTKQIQHHSTGHCNPSMKARFTALRSLAVLFIVFTSYFLTILITIIGTLFDKRCWLWVWEAFVYAFILMHSTSLMLSSPTLKRILKGKC

PTMs - Q9NYV7 As Substrate

Q9NYV7

Site	PTM Type	Source
S51	Phosphorylation	Uniprot
S55	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
Y239	Phosphorylation	Uniprot

Research Backgrounds

Q9NYV7_T2R16_HUMAN

Function:

Gustducin-coupled receptor implicated in the perception of bitter compounds in the oral cavity and the gastrointestinal tract. Signals through PLCB2 and the calcium-regulated cation channel TRPM5.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in a subset of gustducin-positive taste receptor cells of the tongue. Expressed in circumvallate papillae and testis.

Subunit Structure:

Interacts with RTP3 and RTP4.

Family&Domains:

Belongs to the G-protein coupled receptor T2R family.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

TAS2R16 Antibody - #DF5160