TAS2R14 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from MCF-7 cells, using TAS2R14 antibody.

Product:	TAS2R14 Antibody
Catalog:	DF5159
Description:	Rabbit polyclonal antibody to TAS2R14
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	38 KD; 36kD(Calculated).
Uniprot:	Q9NYV8
RRID:	AB_2837508

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

TAS2R14 Antibody detects endogenous levels of total TAS2R14.

RRID:

AB_2837508
Cite Format: Affinity Biosciences Cat# DF5159, RRID:AB_2837508.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

T2R14; T2R14_HUMAN; TAS2R14; Taste receptor family B member 1; Taste receptor type 2 member 14; Taste receptor, family B, member 1; Taste receptor, type 2, member 14; TRB1;

Immunogens

Immunogen:

Uniprot:

Q9NYV8(T2R14_HUMAN) >>Visit HPA database.

Gene(ID):

TAS2R14(50840)

Expression:

Q9NYV8 T2R14_HUMAN:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis (PubMed:16720576).

Sequence:

Q9NYV8 T2R14_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGGVIKSIFTFVLIVEFIIGNLGNSFIALVNCIDWVKGRKISSVDRILTALAISRISLVWLIFGSWCVSVFFPALFATEKMFRMLTNIWTVINHFSVWLATGLGTFYFLKIANFSNSIFLYLKWRVKKVVLVLLLVTSVFLFLNIALINIHINASINGYRRNKTCSSDSSNFTRFSSLIVLTSTVFIFIPFTLSLAMFLLLIFSMWKHRKKMQHTVKISGDASTKAHRGVKSVITFFLLYAIFSLSFFISVWTSERLEENLIILSQVMGMAYPSCHSCVLILGNKKLRQASLSVLLWLRYMFKDGEPSGHKEFRESS

PTMs - Q9NYV8 As Substrate

Q9NYV8

Site	PTM Type	Source
T49	Phosphorylation	Uniprot
Y121	Phosphorylation	Uniprot
N153	N-Glycosylation	Uniprot
T164	Phosphorylation	Uniprot
S170	Phosphorylation	Uniprot
T173	Phosphorylation	Uniprot
S223	Phosphorylation	Uniprot
T224	Phosphorylation	Uniprot
Y300	Phosphorylation	Uniprot
S308	Phosphorylation	Uniprot

Research Backgrounds

Q9NYV8_T2R14_HUMAN

Function:

Receptor that may play a role in the perception of bitterness and is gustducin-linked. May play a role in sensing the chemical composition of the gastrointestinal content. The activity of this receptor may stimulate alpha gustducin, mediate PLC-beta-2 activation and lead to the gating of TRPM5 (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein.

Tissue Specificity:

Expressed in subsets of taste receptor cells of the tongue and palate epithelium and exclusively in gustducin-positive cells. Expressed in testis.

Family&Domains:

Belongs to the G-protein coupled receptor T2R family.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

References

1). Biomimetic in vitro respiratory system using smooth muscle cells on ECIS chips for anti-asthma TCMs screening. Analytica Chimica Acta, 2021 (PubMed: 33926702) [IF=6.2]

Application: IF/ICC Species: Mouse Sample: ASM cells

Fig. 2. Identification of TAS2Rs in mouse ASM cells. (A) RT-qPCR of mTAS2Rs expression in mouse ASM cells, with b-actin as internal control. Results were normalized to expression of the Adora1. Data are collected from three independent experiments. (B) Immunostaining with DAPI (blue), anti-TAS2R10, and anti-TAS2R14 receptor antibody (red), scale bar ¼ 20 mm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

2). Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2. Food & Function, 2021 (PubMed: 34881772) [IF=6.1]

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TAS2R14 Antibody - #DF5159