RHO Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/6

Western blot analysis of extracts from A549 cells, using RHO antibody.

Western blot analysis of extracts from Mouse muscle, using RHO Antibody.

DF5046 at 1/100 staining Mouse testis tissue by IHC-P.

DF5046 staining Hela cells by IF/ICC.

DF5046 staining A549 cells by IF/ICC.

Fig.

Product:	RHO Antibody
Catalog:	DF5046
Description:	Rabbit polyclonal antibody to RHO
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Rabbit, Dog
Mol.Wt.:	38 KD; 39kD(Calculated).
Uniprot:	P08100
RRID:	AB_2837405

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Rabbit(92%), Dog(83%)

Clonality:

Polyclonal

Specificity:

RHO Antibody detects endogenous levels of total RHO.

RRID:

AB_2837405
Cite Format: Affinity Biosciences Cat# DF5046, RRID:AB_2837405.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CSNBAD1; MGC138309; MGC138311; OPN 2; OPN2; opsd; OPSD_HUMAN; opsin 2; Opsin 2 rod pigment; Opsin-2; Opsin2; Retinitis Pigmentosa 4; Retinitis pigmentosa 4 autosomal dominant; RHO; Rhodopsin; RP 4; RP4;

Immunogens

Immunogen:

A synthesized peptide derived from human RHO, corresponding to a region within C-terminal amino acids.

Uniprot:

P08100(OPSD_HUMAN) >>Visit HPA database.

Gene(ID):

RHO(6010)

Expression:

P08100 OPSD_HUMAN:

Rod shaped photoreceptor cells which mediate vision in dim light.

Sequence:

P08100 OPSD_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Dog

Bovine

Sheep

Chicken

Horse

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08100 As Substrate

P08100

Site	PTM Type	Enzyme	Source
N15	N-Glycosylation		Uniprot
Y136	Phosphorylation		Uniprot
Y301	Phosphorylation		Uniprot
Y306	Phosphorylation		Uniprot
S334	Phosphorylation	Q15835 (GRK1) , P17252 (PRKCA)	Uniprot
T336	Phosphorylation	P17252 (PRKCA)	Uniprot
S338	Phosphorylation	Q15835 (GRK1)	Uniprot
T340	Phosphorylation		Uniprot
S343	Phosphorylation	Q15835 (GRK1)	Uniprot

Research Backgrounds

P08100_OPSD_HUMAN

Function:

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins. Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling.

PTMs:

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region (By similarity). After activation by light, phosphorylated by GRK1 (in vitro).

Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal.

Subcellular Location:

Membrane>Multi-pass membrane protein. Cell projection>Cilium>Photoreceptor outer segment.
Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia.

Tissue Specificity:

Rod shaped photoreceptor cells which mediate vision in dim light.

Subunit Structure:

Homodimer (By similarity). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (By similarity). Interacts with GRK1. Interacts (phosphorylated form) with SAG. Interacts with GNAT1. Interacts with GNAT3. SAG and G-proteins compete for a common binding site. Interacts with PRCD; the interaction promotes PRCD stability (By similarity).

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Research Fields

· Organismal Systems > Sensory system > Phototransduction.

References

1). Exposure to blue light stimulates the proangiogenic capability of exosomes derived from human umbilical cord mesenchymal stem cells. Stem Cell Research & Therapy, 2019 (PubMed: 31779691) [IF=7.5]

Application: WB Species: Human Sample: mesenchymal stem cells

Fig. 1 Characterization of MSC photoreceptors for blue/red light irradiation. a Schema of the LED photosystem. b Real-time PCR screening of the basal and stimulated expression of opsins as indicated (OPN4 constitutional expression level was selected as the control group, fold-change = 1). c Proteins were extracted from P2, P4, and P6 passages of MSCs and subjected to immunoblotting. d Proteins were extracted from P4 passage of MSCs with blue/red light illumination and subjected to immunoblotting. e Immunofluorescence staining of the indicated photoreceptors in MSCs

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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RHO Antibody - #DF5046